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Producing method of collagen-chitosan-beta-tricalcium phosphate spherical honeycombed grain material

A technology of tricalcium phosphate and porous particles, used in dental preparations, pharmaceutical formulations, dental prostheses, etc., can solve the problems that the strength and degradation speed cannot meet the needs of tissue engineering cell culture, increase the mechanical strength of the carrier, etc. Good flow and particle size uniformity

Inactive Publication Date: 2012-10-24
JIANGSU HAIJIAN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Pure collagen or chitosan carriers are difficult to meet the needs of tissue engineering cell culture in terms of strength and degradation speed, but the composite of collagen and chitosan increases the mechanical strength of the carrier

Method used

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  • Producing method of collagen-chitosan-beta-tricalcium phosphate spherical honeycombed grain material
  • Producing method of collagen-chitosan-beta-tricalcium phosphate spherical honeycombed grain material
  • Producing method of collagen-chitosan-beta-tricalcium phosphate spherical honeycombed grain material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Weigh collagen and chitosan according to the mass ratio of collagen: chitosan as 1:9, and weigh nano-β-phosphate according to the mass ratio of β-tricalcium phosphate: collagen and chitosan total amount as 1:2 Tricalcium

[0023] (2) adding the collagen and chitosan weighed in the first step into an acetic acid solution with a mass concentration of acetic acid of 2% to prepare a collagen-chitosan-acetic acid solution with a mass concentration of 3 to 22%, adding nanometer β-phosphoric acid tris Calcium, fully stirred to obtain a suspension;

[0024] (3) Add the prepared collagen-chitosan-β-tricalcium phosphate suspension from the feeding port 1 to the storage tank 4, and control the pressure in the storage tank 4 to be 0.08~0.5MPa to ensure that the collagen-shell The polysaccharide-β-tricalcium phosphate suspension flows out uniformly at a rate of 10-60 drops / min from a catheter with a diameter of φ0.5-3 mm. The collagen-chitosan-β-tricalcium phosphate suspension...

Embodiment 2

[0027] (1) Weigh collagen and chitosan according to the mass ratio collagen: chitosan is 9:1, weigh nano-β-phosphate according to the mass ratio of β-tricalcium phosphate: collagen and chitosan total amount is 1:5 Tricalcium;

[0028] (2) adding the collagen and chitosan weighed in the first step into an acetic acid solution with a mass concentration of acetic acid of 4% to prepare a collagen-chitosan-acetic acid solution with a mass concentration of 3 to 22%, adding nanometer β-phosphoric acid tris Calcium, fully stirred to obtain a suspension;

[0029] (3) The prepared collagen-chitosan-beta-tricalcium phosphate suspension is added to the storage tank 4 from the feeding port 1, and the pressure in the control storage tank 4 is 0.08~0.5MPa to ensure that the collagen-chitosan-beta-tricalcium phosphate suspension is Chitosan-β-tricalcium phosphate suspension flows out uniformly at a rate of 10-60 drops / min from a catheter with a diameter of φ0.5-3mm. The collagen-chitosan-β-...

Embodiment 3

[0032] (1) Take collagen and chitosan by mass ratio collagen: chitosan is 5:4, weigh nano-β-phosphate according to the mass ratio of β-tricalcium phosphate: collagen and chitosan total amount is 4:1 Tricalcium;

[0033] (2) adding the collagen and chitosan weighed in the first step into an acetic acid solution with a mass concentration of acetic acid of 1% to prepare a collagen-chitosan-acetic acid solution with a mass concentration of 3 to 22%, adding nanometer β-phosphoric acid tris Calcium, fully stirred to obtain a suspension;

[0034](3) The prepared collagen-chitosan-beta-tricalcium phosphate suspension is added to the storage tank 4 from the feeding port 1, and the pressure in the control storage tank 4 is 0.08~0.5MPa to ensure that the collagen-chitosan-beta-tricalcium phosphate suspension is Chitosan-β-tricalcium phosphate suspension flows out uniformly at a rate of 10-60 drops / min from a catheter with a diameter of φ0.5-3 mm. The collagen-chitosan-β-tricalcium phos...

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Abstract

The invention provides a spherical porous granular material of collagen-chitosan-beta-tricalcium phosphate with uniformly distributed grain diameter, a method and a device for preparing the same. The collagen-chitosan-beta-tricalcium phosphate spherical porous grains prepared contain a plurality of mutually communicated micropores, have high specific surface, are favorable for the flowing of adhesional tissue fluid of cells; have uniform particle size distribution and do not need screen separation treatment to obtain the granular materials with the same grain diameter; according to the surface tension formula, the collagen-chitosan-beta-tricalcium phosphate spherical porous grains prepared by the method have good homogeneity of the grain diameter, the spherical granular material with the same grain diameter has the largest interparticle porosity after being filled, so the collagen-chitosan-beta-tricalcium phosphate composite spherical porous granular material is favorable for the flowing of the tissue liquid and migration and growth of the cells after being filled.

Description

1. Technical field [0001] The invention belongs to a spherical granular material used in the field of biomedicine, in particular to a natural polymer collagen-chitosan-β-tricalcium phosphate composite spherical porous granular material and a preparation method and device thereof. 2. Background technology [0002] Prior art: Collagen or collagen is a kind of biopolymer synthesized by animal cells, widely present in animal bones, tendons, cartilage, skin and other connective tissues, accounting for about 30% of the total protein of mammals , is an important extracellular matrix component of the human body. Collagen has good biological properties and can be used as a support for tissues. It has a major impact on the normal function of cells, tissues and even organs and the repair of trauma. A large number of research results show that the performance of collagen can be used as a culture substrate for epidermal cells, fibroblasts, corneal cells, chondrocytes and bone cells. In...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L27/20A61L27/12A61L27/56A61K6/097A61K6/08A61K6/033A61K6/838A61K6/884A61K6/898
Inventor 董寅生储成林盛晓波郭超林萍华浦跃朴
Owner JIANGSU HAIJIAN