Method for simultaneously extracting lipid and protein from microalgae

A technology for extracting oil and protein, which is applied in the preparation methods of peptides, chemical instruments and methods, and oil/fat production. Easy, high fat and protein yield, avoids the effect of drying the algal flour

Active Publication Date: 2009-05-13
云南中科雨虹生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when it is actually applied to microalgal cells such as chlorella with thicker cell walls and higher cell wall protein content, or diatom cells with siliceous shells such as Phaeodactylum tricornutum, the effect is not very good. , and the explosion when a large amount of high-pressure air is released instantaneously will cause the wet algae cells to splash, which is not conducive to cell collection
In addition, because oilseeds generally have high oil content and are dry seeds, when the aqueous method is used for oil extraction of oilseeds

Method used

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  • Method for simultaneously extracting lipid and protein from microalgae

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Weigh 2000g of wet algae mud of Phaeodactylum tricornutum, add 2400g of distilled water to dissolve the algal cells, and adjust the pH value of the cell solution to 10. The microalgae cell solution was passed into a mechanically stirred reaction tank, and saturated steam was introduced, and the temperature in the tank was raised and maintained to 120° C., the stirring speed was 150 rpm, and the reaction was carried out for 30 minutes. Centrifuge the microalgae cell slurry at 4000rpm for 10 minutes to remove the microalgae cell residues to obtain a mixed solution, adjust its pH value to 7.0, use a small vortex type hydrocyclone to separate the mixed solution, and collect the upper oil phase to obtain algae Oil 45g 0. The protein-containing aqueous solution was filtered with a nanofiltration membrane (MWCO 300Dalton) to obtain a protein concentrate, and the pH value was adjusted to 2.5 with hydrochloric acid. After standing for 20 minutes, the supernatant was removed to o...

Embodiment 2

[0021] Weigh 2000g of wet algae mud of Phaeodactylum tricornutum, add 7000g of distilled water to dissolve the algal cells, and adjust the pH value of the cell solution to 14. The microalgae cell solution was passed into a mechanically stirred reaction tank, and saturated steam was introduced, and the temperature in the tank was raised and maintained to 140° C., the stirring speed was 150 rpm, and the reaction was carried out for 25 minutes. Centrifuge the microalgae cell slurry with a centrifuge at 4000rpm for 10 minutes to remove the microalgae cell residues to obtain a mixed solution, adjust its pH value to 6.8, use a small vortex type hydrocyclone to separate the mixed solution, and collect the upper oil phase to obtain algae. 47 grams of oil. The protein-containing aqueous solution was filtered with a nanofiltration membrane (MWCO 300Dalton) to obtain a protein concentrate, and the pH value was adjusted to 3.0 with hydrochloric acid. After standing for 20 minutes, the sup...

Embodiment 3

[0023] Weigh 2000g of wet algae mud of Phaeodactylum tricornutum, add 3000g of distilled water to dissolve the algal cells, and adjust the pH value of the cell solution to 7.5. The microalgae cell solution was passed into a mechanically stirred reaction tank, and the temperature in the tank was raised and maintained to 110° C. by passing saturated steam, and the stirring speed was 150 rpm, and the reaction was carried out for 10 min. Centrifuge the microalgae cell slurry with a centrifuge at 4000rpm for 10 minutes to remove the microalgae cell residues to obtain a mixed solution, adjust its pH value to 5.0, use a small vortex type hydrocyclone to separate the mixed solution, and collect the upper oil phase to obtain algae. 42 grams of oil. The protein-containing aqueous solution is filtered with a nanofiltration membrane (MWCO 300Dalton) to obtain a protein concentrate, and its pH value is adjusted to 4.0 with hydrochloric acid. After standing for 20 minutes, the supernatant i...

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Abstract

The invention relates to a method for simultaneously extracting grease and proteins from microalgae, which takes wet algae mud as a raw material, adjusts the pH value to be alkaline or slightly alkaline, and performs wall breaking of microalgae cells and dissolution of the grease and the proteins through vapor. The method is as follows: dissolved microalgae slurry is filtered for removal of cell residue, and a mixture of the grease and the proteins is obtained; a hydrocyclone is utilized for oil-water separation, and microalgae grease is obtained; and a protein aqueous solution is subjected to nano-filtration, condensation, isoelectric precipitation and freeze drying or spray drying, and protein powder is obtained. The method utilizes the water-bearing microalgae cells as the raw material, saves the energy consumption for drying the algae cells, adopts a slightly alkaline or alkaline algae cell liquid to realize vapor wall breaking and simultaneous dissolution of the grease and the proteins, and has good wall breaking effect, high dissolution rate, low energy consumption and easily widened process. The method adopts vortex hydrocyclone separation to well solve the problem of difficult oil-water separation under the condition of low phase ratio and realize simultaneous extraction of the microalgae grease and the proteins. Moreover, the method has the advantages of simple process, economy, low energy consumption, and easy realization of industrialization.

Description

technical field [0001] The invention belongs to the technical field of microalgae bioengineering, and in particular relates to a method for simultaneously extracting oil and protein from microalgae, especially for microalgae biodiesel refining and microalgae protein polygeneration. Background technique [0002] Microalgae are aquatic photoautotrophic microorganisms that use light energy to produce CO 2 and H 2 O acts as a substrate for cell growth, and synthesizes a large amount of proteins, triglycerides (TAGs), pigments, polysaccharides and other substances. Microalgae protein has important application value in food, medicine, feed and other fields, and triglycerides (TAGs), including unsaturated glycerides (DHA, EPA) can not only be used as important nutritional security products, but also can be used as It is an important source of oil raw material for biological liquid fuels (such as biodiesel). Especially with the worldwide shortage of fossil raw materials such as p...

Claims

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Application Information

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IPC IPC(8): C11B1/00C07K1/34C07K1/14
Inventor 刘天中江怀真高莉丽张维陈昱
Owner 云南中科雨虹生物科技有限公司
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