Clone and expression of alpha-glucosidase gene

A glucosidase and gene technology, which is applied to the DNA sequence encoding Aspergillus niger WX-07AGLU enzyme and the field of expression thereof, can solve problems such as the recovery and use of unfavorable recombinant α-glucosidase
CN101434943BInactive Publication Date: 2010-12-22JIANGNAN UNIV
0 Cites 3 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2010-12-22
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses clone and expression of an Alpha-glucosidase (abbreviated as AGLU enzyme) gene and belongs to the fields of enzyme gene engineering and enzyme engineering. The invention obtains the aglu gene with SEQ ID NO of 1 through an Aspergillus niger (A.niger) WX-07 total DNA, and aglu cDNA uses an expression vector of plasmid pPIC9K and an expression parasitifer of pichia stipitis (P.pastoris), thus realizing extracellular dissolubility expression of the aglu gene; and the aglu cDNA totally has 2880 nucleic acid and encoding of 960 amino acid, constructs eukaryotic expression plasmid and transforms Pichia pastoris KM71 for AGLU enzyme expression. The recombinant enzyme has transnucleosidation activity, can generate isomalto oligosaccharide through maltose transnucleosidation, is applicable to the requirements of industrial application including food, feeding stuffs and medicaments and the like, and can be applied into the industrial production of isomalto oligosaccharide.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field

[0001] The cloning and expression of an alpha-glucosidase (AGLU enzyme for short) gene belongs to the field of enzyme genetic engineering and enzyme engineering. Specifically, it relates to a DNA sequence encoding Aspergillus niger WX-07AGLU enzyme and its expression. Background technique

[0002] α-glucosidase (α-glucosidase, AGLU enzyme for short, EC 3.2.1.20), also known as α-D-glucoside hydrolase, can cleave α-1,4 glycosidic bonds from the non-reducing ends of oligosaccharides. Glucose is released and the free glucose groups are transferred to other sugar substrates in the form of α-1,6 glycosidic bonds to form non-fermentable functional oligosaccharides-isomalto-oligosaccharides (IMOs for short, mainly including Isomaltose, panose, isomaltotriose, etc.). Isomaltooligosaccharides are a kind of oligosaccharides containing at least one α-(1,6) glycosidic bond, mainly isomaltose, panose, isomaltose, and a small amount naturally exists in soy sauce and honey. It...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More