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Nano magnetic particle chromatography test paper detection method

A technology of nano-magnetic particles and chromatographic test paper, which is applied in the detection field, can solve the problems of limited sample loading, complicated preparation process, and low detection rate in immunochromatography experiments, so as to promote clinical application, simple preparation process, and low cost Effect

Active Publication Date: 2009-05-27
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] 1. At present, the sensitivity of common colloidal gold or colloidal selenium immunochromatography experiments is lower than that of enzyme-linked immunosorbent assays
[0006] 2. Due to the limitations of its volume and water-absorbing materials, the amount of sample loaded in immunochromatography experiments is limited. Therefore, when there are few target analytes in some samples such as saliva and urine, there will be fewer analytes to be detected due to the limitation of the detection sample volume. While the detection rate is low
[0007] 3. The preparation process is more complicated
The traditional process includes the preparation of detection markers, pretreatment of sample pads and detection marker pads, drying and curing of detection marker spray pads, curing of antigens or antibodies on nitrocellulose membranes, sample pads, detection marker pads, nitric acid The four-layer lap bonding of cellulose film and absorbent pad requires many and complicated procedures

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] HBsAb detection

[0024] a) Weigh 2.51g (0.0093mol) FeCl 3 ·6H 2 O and 1.25g (0.0045mol) FeSO 4 ·7H 2 O was dissolved in 600ml of deionized water, and under the protection of nitrogen at room temperature, 24ml of ammonia water with a concentration of 1.5mol / l was added point by point to the above solution to obtain a black precipitate. Washed 5 times with deionized water by means of magnetic separation, dispersed the magnetic nanoparticles in 12 ml of 0.3 M sodium citrate solution and reacted for 30 minutes.

[0025] Or by pyrolysis method, weigh 0.706g (0.002mol) of iron acetylacetonate (Fe(acac) 3 ) was dissolved in 10ml of dibenzyl ether and 10ml of oleylamine and dehydrated at 110°C for 1 hour, quickly heated to 300°C for 2 hours, added 50ml of ethanol after cooling, and added 0.015mol of sodium citrate solution to react for 30 minutes.

[0026] b) Take 1ml of nano-magnetic particles with a concentration of 0.25mM, add 2.5ml of phosphate buffer solution (pH6.0)...

Embodiment 2

[0030] HBsAg test

[0031] a) Weigh 2.51g (0.0093mol) FeCl 3 ·6H 2 O and 1.25g (0.0045mol) FeSO 4 ·7H 2 O was dissolved in 600ml of deionized water, and under the protection of nitrogen at room temperature, 24ml of ammonia water with a concentration of 1.5mol / l was added point by point to the above solution to obtain a black precipitate, which was washed 5 times with deionized water by means of magnetic separation to obtain magnetic nanoparticles.

[0032] Or by pyrolysis method, weigh 0.706g (0.002mol) of iron acetylacetonate (Fe(acac) 3 ) was dissolved in 10ml dibenzyl ether and 10ml oleylamine and dehydrated at 110°C for 1 hour, heated rapidly to 300°C for 2 hours, and added 50ml ethanol after cooling.

[0033] Take 74mg of nano-magnetic particles obtained by the above two methods and disperse them in 149ml of ethanol and 1ml of water for 30 minutes by ultrasonic dispersion, add 35μl of APTS and stir rapidly for 7h, then magnetically separate and wash to obtain nano-mag...

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Abstract

The invention relates to a nano magnetic particle chromatographic-paper detection method in detection technical field, comprising synthesizing nano black magnetic particles, carrying out the hydroxyl, amino, carboxyl, or sulfhydryl modification on the surface of the nano black magnetic particles, coupling the modified particles with antigen or antibody, freeze-drying the coupled particles and storing for later use, then sequentially overlapping the detection reaction part and the adsorption part on the substrate of the test paper to prepare chromatographic-paper strip, wherein the detection reaction part is coated with an antigen or antibody detection area, and simultaneously coated with corresponding antigen or antibody as reference line, the existence of the related antigen or the antibody can be determined by the color of nano magnetic particles. The detection method has high specificity and detectable rate, can better meet the requirements of the rapid non-destructive in-vitro diagnosis and the detection requirements of large sample containing trace substances to be detected. The method of the invention is simple, rapid, convenient, and easy to promote.

Description

technical field [0001] The invention relates to a method in the technical field of detection, in particular to a detection method of nano magnetic particle chromatography test paper. Background technique [0002] In recent years, nanoparticles have attracted widespread attention due to their unique physical and chemical properties. In particular, magnetic nanoparticles, due to their unique superparamagnetism and a specific surface area that greatly surpasses other materials, have extremely wide applications in the fields of medicine and biology (Anal Bioanal Chem. 2006384(3): 593-600). The superparamagnetism of magnetic nanoparticles allows them to be manipulated under an artificially applied magnetic field; functionalized magnetic nanoparticles can be coupled or modified with a large number of biological molecules or other functionalized materials for cell separation, automated nucleic acid Extraction, gene targeting, drug delivery, magnetic resonance phenomena, and hypert...

Claims

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Application Information

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IPC IPC(8): G01N33/52G01N33/558G01N33/577
Inventor 杨浩黄鹏涂津铭崔大祥
Owner SHANGHAI JIAO TONG UNIV
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