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Gelatin/calcium phosphate composite porous microsphere for bone repair and preparation method and usage of the porous microsphere

A technology of porous microspheres and calcium phosphate, which is applied in the field of preparation of gelatin derivative materials, has achieved wide application value, accelerated bone tissue regeneration, good biocompatibility and the effect of inducing osteogenic activity

Active Publication Date: 2009-06-03
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention prepares composite porous microspheres by co-precipitating soluble calcium salts and phosphorus salts in gelatin emulsion, adding appropriate amount of velvet antler polypeptide or bone morphogenetic protein in the reaction to obtain bone repair composite material and preparation of the composite material The method has not been reported in the literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Accurately weigh 1 gram of gelatin powder into a 100ml beaker, add 5ml of deionized water, place it in a 50±1℃ water bath and stir to dissolve, and then add 0.0025mol required to form calcium phosphate into the beaker Calcium nitrate solid, then add 3mg of velvet antler polypeptide to make a mixed solution.

[0034] 2. Under stirring at 500 rpm, the above solution was slowly added dropwise to 50ml of soybean oil phase dissolved with surfactant (SPAN 80, 1g / 50ml) to form a water-in-oil emulsion of gelatin solution. Keep the reaction temperature at 45±1℃, and continue to stir for 2 hours, then add dropwise Na with a mass concentration of 5% to the emulsion system 2 HPO 4 Solution (Ca / P molar ratio is 5:3).

[0035] 3. After the reaction is over, the reactant at 45±1℃ is quickly transferred to an ice-water bath, kept stirring, and cooled for 40 minutes to gelatinize the gelatin microspheres containing calcium phosphate and form the gelatin microspheres evenly dispersed with c...

Embodiment 2

[0040] 1. Accurately weigh 1 gram of gelatin powder into a 100ml beaker, add 5ml of deionized water, place it in a 50±1℃ water bath and stir to dissolve, then add 0.003mol needed to form calcium phosphate into the beaker Calcium chloride solid, and then add 20mg velvet antler polypeptide to it to make a mixed solution.

[0041] 2. Under stirring at 500 rpm, the above solution was slowly added dropwise to 100ml of olive oil phase with surfactant (SPAN 60-1g / 50ml) to form a water-in-oil emulsion of gelatin solution , Keep the reaction temperature at 45±1℃, and continue to stir for 2 hours, then add dropwise 10% Na in the emulsion system 2 HPO 4 Solution (Ca / P molar ratio is 5:3).

[0042] 3. After the reaction, the reactant at 45°C ± 1°C was quickly transferred to an ice water bath, kept stirring, and cooled for 40 minutes to gelatinize the gelatin microspheres containing calcium phosphate and form a uniform dispersion of the gelatin microspheres. Calcium phosphate and velvet antler...

Embodiment 3

[0047] 1. Accurately weigh 1 gram of gelatin powder into a 100ml beaker, add 5ml of deionized water, place it in a 40±1℃ water bath and stir to dissolve, then add 0.01mol required to form calcium phosphate into the beaker Calcium chloride solid is prepared into a gelatin solution containing a certain calcium ion concentration, and then 20 mg of velvet antler polypeptide is added to it and dispersed evenly.

[0048] 2. Under stirring at a rotation speed of 200 rpm, the above solution was slowly added dropwise to 75ml of tea oil phase dissolved with surfactant (dioctyl sodium succinate (AOT)-1g / 50ml), To form a water-in-oil emulsion of gelatin solution, keep the reaction temperature at 45±1℃, and continue to stir for 2 hours, and then add dropwise 10% Na in the emulsion system. 2 HPO 4 Solution (Ca / P molar ratio is 1.5:1).

[0049] 3. After the reaction, the reactant at 45±1℃ is quickly transferred to an ice water bath, kept stirring, and cooled for 30 minutes to gelatinize the gela...

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Abstract

The invention belongs to the field of the preparation of gelatin derivative materials and in particular relates to a composite porous microsphere material formed by compounding gelatin, calcium phosphate and an antler polypeptide bioactive tissue or bone morphogenic protein (BMP). The composite porous microsphere is formed by uniformly dispersing, in a gelatin composite porous microsphere, the calcium phosphate and active-substance antler polypeptide or bone morphogenic protein which is capable of promoting bone growth; wherein, the weight of the active-substance antler polypeptide or the bone morphogenic protein accounts for millesimal to five percent of the weight of the gelatin; soluble calcium salt which forms the calcium phosphate ranges from 0.0003 to 0.03 mol in each gelatin; and the amount of soluble phosphate causes the ratio between Ca element and P element to range from 1:1 to 2:1. The composite porous microsphere material can also contain crosslinking agent. The composite porous microsphere material is a bone repair biological material which has good biocompatibility and good bone induction activity.

Description

Technical field [0001] The invention belongs to the field of preparation of gelatin derivative materials, and particularly relates to a composite porous microsphere material synthesized from gelatin, calcium phosphate, and a deer antler polypeptide bioactive tissue or bone morphogenetic protein (BMP). Biocompatibility and bone-inducing activity of bone repair biomaterials. Background technique [0002] Gelatin is a product of collagen degradation. It not only maintains some of the unique advantages of collagen as a biological material, but also has no antigenicity compared with collagen, and there is no rejection reaction when implanted in the body; gelatin is cheap and easy to obtain; It is soluble in water; the detection methods of its physical and chemical properties have been developed relatively mature and standardized. Therefore, the composite material of gelatin and calcium phosphate is also a hot spot in the research of bone repair materials. [0003] Traditional gelatin ...

Claims

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Application Information

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IPC IPC(8): A61L27/54A61L27/46
Inventor 陈丽娟郭燕川孙瑞雪史京京邓旭明宋宇
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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