Unlock instant, AI-driven research and patent intelligence for your innovation.

Gene of maize antibacterial protein and use of encoding protein thereof

An antibacterial protein and gene coding technology, applied in the application field of biological pesticides, can solve problems such as economic losses in agricultural production, and achieve the effects of stable antibacterial activity, complete expression, and inhibition of pathogenic bacteria

Inactive Publication Date: 2009-07-08
姜国勇
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Crop diseases are mainly caused by viruses, bacteria and fungi, and their outbreaks have caused huge economic losses to agricultural production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gene of maize antibacterial protein and use of encoding protein thereof
  • Gene of maize antibacterial protein and use of encoding protein thereof
  • Gene of maize antibacterial protein and use of encoding protein thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Such as figure 1 Shown: the expression antimicrobial protein of target gene of the present invention in prokaryotic cell obtains the verification of experiment:

[0042] Clone the strain pET / 91-B108, culture it overnight at 37°C for 12 hours, induce it with IPTG for 3 hours, extract the protein for SDS-PAGE electrophoresis, and obtain the expressed protein with a molecular weight of about 52.1KDa, using MagneHis from Promega Company TM Protein Purification System Kit (V8500), to obtain purified and expressed antibacterial proteins.

Embodiment 2

[0044] Such as figure 2 , 3 , 4, 5, and 6: the antibacterial test of the target gene encoded protein of the present invention to pathogenic bacteria:

[0045] figure 2 Middle: Bacillus subtilis (Bacillus subtilis) test of the protein encoded by the corn antimicrobial protein gene: After three days of cultivation (37°C), Bacillus subtilis grew normally on a plate that did not contain z108 purified protein, and the colony distribution was uniform and the density was relatively high. big (see figure 2 ); while the mixed smeared z108 purified protein culture medium grows rare colonies with a low density, indicating that the z108 purified protein has an inhibitory effect on Bacillus subtilis, and there is no significant growth difference between the two.

[0046] image 3 Middle: Antimicrobial protein gene encoding protein of corn against tomato gray mold (Botrytis cinereaPers.) test: the spores of tomato gray mold (Botrytis cinereaPers.) were cultivated in PDA at 28°C and h...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
Login to View More

Abstract

The present invention relates to the separation of plant disease resistance genes, DNA cloning technology and gene recombination technology, as well as the application technique field of cloning strain in plant bactericide and the like biological pesticides. The invention applies the DNA recombination technique and the molecular biology method to separate an antibacterial protein gene with a length of 906bp from the maize germ, and expresses the encoded protein of the segment of target genes in the Escherichia coli. Test results show that the segment of target genes and its encoded protein can inhibit the growth of Bacillus, prevent the germination of spore such as tomato botrytis and pimiento pine root fungus and elongation of germinal tube, and has a significant role in inhibiting pathogenic bacteria. The protein can be used as a main active component of biological bactericide, for producing non-toxic, non-side-effect and environmental protective biological pesticide.

Description

technical field [0001] The invention relates to the separation of plant disease-resistant genes, DNA cloning technology and gene recombination technology, and the technical field of application of cloned strains in biopesticides such as plant fungicides. Background technique [0002] It is an important research topic in modern pesticide production and biological control research to realize the green and environmental protection of pesticide production and application by using the biological protein synthesized in plant tissue. After millions of years of genetic recombination and natural selection, cultivated plants and their crops have a relatively complete defense system, which can resist the infection and harm from microorganisms. The biochemical reaction process of individual plants produces many compounds that antagonize pathogenic bacteria. ——Biochemical small molecules of various proteins, polypeptides, esters, and phenols, these biochemical substances constitute the d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/70C12N1/21C07K14/415A01N63/02A01P3/00C12R1/19
Inventor 姜国勇
Owner 姜国勇