Trichoderma sp. preparation for preventing and treating soil-borne disease of crop

A technology for soil-borne diseases and Trichoderma, applied in fungicides, biocides, animal repellents, etc., can solve the problems of large dosage, environmental pollution, pesticide residues, etc., and achieve low production costs, short production cycles, The effect of reducing pollution

Inactive Publication Date: 2009-07-22
四川省农业科学院经济作物育种栽培研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent application "A method of preventing and treating plant soil-borne diseases" (publication number: CN1559213A), the method is to use highly toxic and high residue chemical agent methyl bromide 37.5g of the original drug / m 2 Or Weibamu 75g / m 2 Disinfect the soil and then cover with VIF film, not only the dosage is large, it is easy to cause pesticide residues, pollute the environment, and the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The Trichoderma preparation of the control crop soil-borne disease of this example, its preparation method is as follows:

[0042] A. Expansion of strains

[0043] Potato 200g, glucose 20g, agar 18g, the concentration is 1×10 4 After mixing 5ml of PCNB (pentachloronitrobenzene) mother liquor of mg / L, add water to 1000ml to prepare a slant medium; then inoculate the bacterial classification of Trichoderma harzianum strain Th-192 in the slant medium, at 25°C , cultivated for 3 days to obtain an expanded culture of Trichoderma harzianum strain Th-192.

[0044] B. Liquid fermentation culture

[0045] Use corn flour, streptomycin and water to prepare a liquid medium with a mass content of: 3% corn flour, 50 ppm streptomycin, and water as the balance, and then carry out disinfection treatment on the liquid medium, which is to mix the liquid medium and pack it. In the Erlenmeyer flask, sterilize under high temperature and pressure at 121°C and 15 pounds for 30 minutes; afte...

Embodiment 2

[0052] The Trichoderma preparation of the control crop soil-borne disease of this example, its preparation method is as follows:

[0053] A. Expansion of strains

[0054] Potato 200g, glucose 20g, agar 17g, the concentration is 1×10 4 After mixing 5ml of PCNB (pentachloronitrobenzene) mother liquor of mg / L, add water to 1000ml to prepare a slant medium; then inoculate the bacterial classification of Trichoderma harzianum strain Th-192 in the slant medium, and incubate at 28°C , cultivated for 2 days to obtain an expanded culture of Trichoderma harzianum strain Th-192.

[0055] B. Liquid fermentation culture

[0056] Use corn flour, streptomycin and water to prepare a liquid medium with a mass content of 5% corn flour, 55 ppm streptomycin, and water, and then disinfect the liquid medium to mix the liquid medium and pack it. In the Erlenmeyer flask, sterilize under high temperature and pressure at 121°C and 15 pounds for 30 minutes; after cooling, inoculate the expanded cultu...

Embodiment 3

[0063] The Trichoderma preparation of the control crop soil-borne disease of this example, its preparation method is as follows:

[0064] A. Expansion of strains

[0065] Potato 200g, glucose 20g, agar 18g, the concentration is 1×10 4 After mixing 5ml of PCNB (pentachloronitrobenzene) mother liquor of mg / L, add water to 1000ml to prepare slant medium; then inoculate the bacterial classification of Trichoderma harzianum strain Th-192 in the slant medium, at 20 ℃ temperature , cultivated for 3 days to obtain an expanded culture of Trichoderma harzianum strain Th-192.

[0066] B. Liquid fermentation culture

[0067] Use corn flour, streptomycin and water to prepare a liquid culture medium with a mass content of 4% corn flour, 50 ppm streptomycin, and water, then carry out disinfection treatment on the liquid culture medium and mix the liquid medium evenly. In the Erlenmeyer flask, sterilize under high temperature and pressure at 121°C and 15 pounds for 40 minutes; after coolin...

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PUM

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Abstract

The invention discloses a trichoderma preparation for preventing and treating soil-borne diseases of crops and a preparation method thereof. The compositions of the preparation comprise that: a carrier comprises a useful quantity of Trichoderma harzianum strains Th-192 CGMCC NO.2634. The preparation method comprises the steps of propagation of the strains Th-192, liquid and solid fermentation culture, addition of an accelerant, and the like. The preparation has the advantages of wide sources of raw materials, short production period, low cost, large spore yield, convenient use, wide applicable crops and obvious functions of promoting growth and increasing production.

Description

Technical field [0001] The invention relates to a Trichoderma preparation for preventing and treating soil-borne diseases of crops and a preparation method thereof. Background technique [0002] Root rot, bacterial blight, blight, sclerotinia, black shank and other crop soil-borne diseases cause a loss of more than 20% of the annual yield, seriously affecting the yield and quality of crops. Since the pathogenic bacteria of soil-borne diseases can survive in the soil for a long time and spread with the soil along with field farming activities, long-term use of chemical agents is required for prevention and control. The use of chemical pesticides will cause pesticide residues to exceed the standard. This has become the main obstacle for my country's agricultural products to enter the international market. Therefore, there is an urgent need for pollution-free prevention and control technologies in agricultural planting and production to improve the sustainable development of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P3/00C12N1/14C12R1/885
Inventor 曾华兰叶鹏盛何炼韦树谷刘朝辉李琼英张骞方
Owner 四川省农业科学院经济作物育种栽培研究所
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