Notoginsen triterpenes capsule as well as preparation method thereof and method for measuring content
A technology of Panax notoginseng saponins and capsules, which can be used in capsule delivery, measuring devices, and pharmaceutical formulations, etc. It can solve the problems of affecting the detection method and not applicable to the detection of the content of Panax notoginseng saponins in capsules, and achieves low operating costs and improved quality control. The effect of good level and powder fluidity
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Embodiment 1
[0022] Take notoginseng, moderately pulverize it, add 2.5 times the volume fraction of 85% ethanol solution, heat and reflux for extraction and concentration for 10 hours, and concentrate under reduced pressure until it has no alcohol smell. The dregs are directly passed through the steam to recover ethanol. Dilute the concentrated ointment with purified water to 0.25g crude drug / ml, sink in water for 18 hours, and filter with filter paper. The filtrate is adsorbed by D-101 macroporous adsorption resin through the column at a flow rate of 1.3BV / h, and then purified with 1 times the amount of resin Rinse the resin column with water, then soak the resin with 1 times the amount of 85% ethanol solution for 30 minutes, and then use 3 times the amount of 85% ethanol solution to elute at a flow rate of 1.0BV / h. The eluate flowing out from the D-101 resin directly passes through the D900 decolorizing resin at a flow rate of 6.5BV / h. When the resin surface is exposed, add 85% ethanol s...
Embodiment 2
[0026] Panax notoginseng saponins mainly contain ginsenoside Rg 1 , Ginsenoside Rb 1 and notoginsenoside R 1 , the applicant refers to the content determination method of Panax notoginseng saponins injection provided in the quality standard of WS-10986 (ZD-0986)-2002 (see Table 1), and the content of the ginseng medicinal materials provided in the Chinese Pharmacopoeia 2005 edition. Content determination method (see table 2) detects Panax notoginseng saponins capsule of the present invention, and the result shows, the former can not effectively separate the chromatographic peak of active ingredient and impurity peak, and serious drift occurs in baseline; The latter ginsenoside Rb 1Chromatographic peaks have large differences in peak areas due to the use of different chromatographic columns, which shows that ginsenoside Rb 1 The separation of chromatographic peaks is greatly affected by the chromatographic column, and the chromatographic conditions are not durable. After adj...
Embodiment 3
[0034] 1 to 10 batches of samples prepared in Example 1 were detected by the following content determination method.
[0035] Determination according to high performance liquid chromatography (Chinese Pharmacopoeia 2005 edition one attached VI D).
[0036] Chromatographic conditions and system suitability test use octadecylsilane bonded silica gel as filler, and the number of theoretical plates is based on ginsenoside Rg 1 Peak calculation should not be lower than 6000, using acetonitrile-water as the mobile phase, wherein acetonitrile is mobile phase A, water is mobile phase B, and the detection wavelength is 203nm, including column balance, sample loading, and elution steps. In the step, first use 19% by volume of phase A and 81% of phase B as the mobile phase to elute 2 to 6 column volumes; then gradually increase the volume percentage of mobile phase A to elute 1 to 3 column volumes to The volume percentage of mobile phase A is 29% and the volume percentage of mobile phas...
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Abstract
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