Preparation method of high-purity magnolia bark total-phenol

Abstract

The invention relates to a method for preparing high purity magnolia P.E.. The method is to cut root or root bark of young magnolia officinalis planted for 2 to 5 years into small segments, carry out seepage by an alkaline solution, regulate a seepage solution by acid until the solution is slight acidic, precipitate by Soxhlet extraction of a lower organic solvent, concentrate to obtain crystal, and dry the crystal to obtain a magnolia officinalis extract product with high total phenolic content. The magnolia P.E. prepared by the method has the advantages of high content, low cost, simple preparation process and small pollution.

Description

technical field [0001] The invention relates to a plant extract, in particular to a preparation method of total magnolol obtained from magnolia bark root or root bark. technical background [0002] The raw material used in the traditional preparation of magnolol is the dry bark or branch bark of the perennial Magnolia officinalis Rehd.et Wils. or Magnolia officinalis Rehd.et Wils.var.bilobaRehd.et Wils. The raw materials are extracted and refined to obtain a high-content magnolia total phenol extract. The main active ingredients in Magnolia officinalis are magnolol, honokiol, isomagnanolol, tetrahydromagnokiol, magnolol, etc. Among them, magnolol and honokiol have the highest content. Chinese Pharmacopoeia It is stipulated that the content of these two phenols can be used medicinally if the content of these two phenols reaches more than 2% in the medicinal material. Generally, the content of the trunk bark of a tree that has grown for about 15 years is between 3-5%, and the...

AI Technical Summary

Problems solved by technology

The medicinal materials used are all Magnolia officinalis raw materials processed by traditional methods, including branch officinalis, root bark, etc. In order to reduce the production cost, it is even necessary to make the total phenolic content of Magnolia officinalis over 20 years old at 8%, which often causes the destruction of wild resources

Although the ethanol extraction method can obtain a higher extraction rate in the initial extraction, the content of the obtained product is not high, and it is difficult to follow-up to the high-purity product; supercritical CO 2 The extraction method and equipment are expensive, the initial investment is large, the cost is too high, and it is difficult to realize large-scale industrial production; although silica gel column chromatography has a good separation effect on active ingredients and high product yield, it is difficult for large-scale industrial production. It is difficult to realize; although the macroporous resin method can realize industrial production, the cycle is long and the cost is high

TLC and HPLC methods are only suitable for small-scale preparation and detection in the laboratory, and cannot be used for large-scale industrial production applications; crude products obtained by alkali extraction and alkali extraction and acid precipitation methods are extracted with petroleum ether and cyclohexane by reflux At this time, the extraction rate of active ingredients is not high, the amount of solvent consumed is large, and the repeated transfer of a large amount of solvents has great potential safety hazards

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