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Novel methods for simultaneously extracting tea polyphenol, tea polysaccharide and caffeinum from tea

A technology for tea polyphenols and caffeine, applied in the field of simultaneous extraction of tea polyphenols, tea polysaccharides, and caffeine, can solve the problems of toxic safety factor, low extraction rate, low product purity, etc., to ensure safety and reliability, The effect of improving extraction rate and purity and high resource utilization

Inactive Publication Date: 2009-08-19
林峰 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned methods all have shortcomings in varying degrees. The organic solvent extraction method requires a large amount of solvents, and solvents such as methanol, ethanol, acetone, methylene chloride, and ethyl acetate are commonly used, and there are toxic solvent residues, which are not easily accepted by the food and pharmaceutical industries; The metal ion precipitation method is complicated to operate, the extraction rate is low, and the metal ion residue is high, which affects the product quality; the resin adsorption method has low product purity due to the low selectivity of the resin, and all use toxic or poor safety eluents; supercritical The production cost of fluid extraction method is high, and the technology is lack of maturity and reliability
In addition: they all have a common disadvantage: extractants and eluents use toxic or low-safety solvents (the best are also ethanol)
Chinese patent (200510042629.3) reported a method for extracting tea polyphenols by-product caffeine and tea polysaccharides from tea leaves, using enzymatic extraction, the method is good but not comprehensive, did not fully play the function of enzymatic hydrolysis, useful ethanol precipitation, sulfuric acid washing Desorption and trichloroacetic acid precipitation will have an impact on product safety

Method used

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  • Novel methods for simultaneously extracting tea polyphenol, tea polysaccharide and caffeinum from tea

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Use 10kg of tea leaves (low-grade tea, coarse old tea leaves, discarded branches and leaves pruned in tea gardens, waste leftovers in tea production), after crushing to 80-100 mesh, add 150kg of water, heat up to 50 degrees, add 30g of compound enzyme (neutral Protease: papain: trypsin weight ratio 2:1:1), keep the temperature constant, extract for 60 minutes, add water 10kg to the tea dregs after filtering, keep the temperature constant at 50 degrees, then extract for 30 minutes. Combine the two leached solutions, add protein remover (sodium chloride) 5g, and stir for 30 minutes. At room temperature to 40 degrees, use 0.1μm-0.3μm ultrafiltration membrane for ultrafiltration, the inlet and outlet pressure ratio is 0.2-0.4mpa, the cut-off flow is 10ml / min, and the ultrafiltration membrane has a molecular weight of 5000- 10000, because the filtered tea residue has been enzymatically hydrolyzed, it can be used as animal feed, organic compound fertilizer or foot soak agent,...

Embodiment 2

[0038] Use 10kg of tea leaves (low-grade tea, coarse old tea leaves, discarded branches and leaves pruned in tea gardens, waste leftovers in tea production), after crushing to 80-100 mesh, add 160kg of water, heat up to 50 degrees, add 25g of compound enzyme (neutral Protease: xylanase: trypsin weight ratio 2:1:1), keep the temperature constant, extract for 60 minutes, add water 10kg to the filtered tea dregs, keep the temperature constant at 50 degrees, and then extract for 30 minutes. Combine the two leached solutions, add 5 g of protein remover (sodium chloride), and stir for 30 minutes. Cool at room temperature to 40 degrees, use 0.1μm-0.3μm ultrafiltration membrane for ultrafiltration, the inlet and outlet pressure ratio is 0.2-0.4mpa, the interception flow is 10ml / min, and the ultrafiltration membrane has a molecular weight of 10000—15000 (the filtered tea residue is enzymatically hydrolyzed and used as animal feed, organic compound fertilizer or foot soak agent, etc., t...

Embodiment 3

[0041] Use 10kg of tea leaves (low-grade tea, coarse old tea leaves, discarded branches and leaves pruned in tea gardens, waste leftovers in tea production), after crushing to 80-100 mesh, add 170kg of water, heat up to 50 degrees, add 35g of compound enzyme (neutral Protease: papain: dextranase weight ratio 2:1:1), keep the temperature constant, leaching for 60 minutes, add 10-15kg of water to the filtered tea dregs, keep the temperature at 50 degrees, and then leaching for 30 minutes minute. Combine the two leached solutions, add 5 g of protein remover (sodium chloride), and stir for 30 minutes. At room temperature to 40 degrees, use 0.1μm-0.3μm ultrafiltration membrane for ultrafiltration, the inlet and outlet pressure ratio is 0.2-0.4mpa, the interception flow is 10ml / min, and the molecular weight of the ultrafiltration membrane is 15000- 20000 (filtered tea dregs have been enzymatically hydrolyzed and used as animal feed, organic compound fertilizer or foot soak agent, e...

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Abstract

The invention provides a new method for simultaneously extracting tea polyphenol, tea polysaccharide and caffeine from tea leaves. The method comprises the following steps: extracting by enzymolysis, ultrafiltering, adsorbing the tea polyphenol and the caffeine with resin, obtaining the caffeine and the tea polyphenol by elution, and obtaining the tea polysaccharide by reverse osmosis. The method organically combines a new concept of enzymolysis extraction, membrane separation and resin adsorption, does not use any organic solvent (including ethanol), only uses water for precipitation and elution, and has the advantages of pollution-free, safe and reliable production, low cost, high product purity and high yield.

Description

technical field [0001] The invention belongs to the technical field of tea deep processing, and in particular relates to a new method for simultaneously extracting tea polyphenols, tea polysaccharides and caffeine from tea leaves. Background technique [0002] my country is the first country to discover and utilize tea trees, and is known as the motherland of tea. Historical records show that our ancestors began to cultivate and utilize tea trees as early as 3,000 years ago. Currently, the planting area of ​​tea gardens and tea production rank first in the world. A large number of low-grade tea, coarse old tea, discarded branches and leaves pruned, and leftovers discarded in tea production have no direct market outlets, but they contain many resources that can be used. In my country's tea products, deep-processing products only account for about 3%, and there are problems such as backward processing technology, low technical content and low added value of products. Among th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C08B37/00C07D473/12C12P19/04
Inventor 林峰黄荣华陈贤峰
Owner 林峰
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