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Method for preparing amino functional magnetic fluorescent coding microsphere with double-nucleocapsid structure

A technology of amino functionalization and core-shell structure, which is applied in the field of preparation of magnetic fluorescent coded microspheres, can solve the problems of cumbersome operation, no agent, high product price, etc., and achieve the effect of high stability and high fluorescence intensity

Inactive Publication Date: 2009-09-16
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the products of Dynal Company are relatively mature and have been commercialized. However, because the operation is extremely cumbersome and requires special equipment and technology, the products of Dynal Company are expensive and have no agents in China.

Method used

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  • Method for preparing amino functional magnetic fluorescent coding microsphere with double-nucleocapsid structure
  • Method for preparing amino functional magnetic fluorescent coding microsphere with double-nucleocapsid structure
  • Method for preparing amino functional magnetic fluorescent coding microsphere with double-nucleocapsid structure

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Prepare the fluorescence intensity ratio (I QDs1 / I QDs2 ) is a 3:1 amino-functionalized double-core-shell magnetic fluorescently encoded microsphere

[0035] Step a) preparation of superparamagnetic ferric oxide nanoparticles:

[0036] The preparation of ferroferric oxide nanoparticles adopts the most commonly used co-precipitation method. Weigh 0.54g of FeCl 3 ·6H 2 O and 0.2 g FeCl 2 4H 2 O was dissolved in 10 ml of deionized water through nitrogen and deoxygenation to obtain a mixed solution. Get 70ml of deionized water in a 250ml three-necked bottle for nitrogen deoxygenation for 30 minutes, add 10ml of concentrated ammonia water with a mass fraction ratio of 28%, add the above-mentioned iron salt mixed solution rapidly under vigorous stirring, React at room temperature for 30 minutes. After the reaction is completed, use a permanent magnet (magnetic strength is 0.1T) to separate the black solid from the reaction solution, and the obtained solid can be wash...

Embodiment 2

[0045] Prepare the fluorescence intensity ratio (I QDs1 / I QDs2 ) is a 1:1 amino functionalized double core-shell structure magnetic fluorescent encoded microspheres

[0046] Step a) the preparation of superparamagnetic iron ferric oxide nanocrystals is the same as step a) of Example 1;

[0047] Step b) The preparation of the CdTe quantum dots modified by mercaptosuccinic acid with high fluorescence intensity is the same as step b) of Example 1;

[0048] Step c) monochromatic magnetic fluorescent microspheres (Fe 3 o 4 -QDs 1 / SiO 2 ) with the step c) of embodiment 1;

[0049] Step d) prepare fluorescence intensity ratio (I QDs1 / I QDs2 ) is a 1:1 amino-functionalized double core-shell structure magnetic fluorescent encoded microspheres: add 7.5ml cyclohexane, 1.77ml Triton X-100, 1.8ml n-hexanol, 200μL QDs in a 50ml Erlenmeyer flask 2 (0.0006 μmol) aqueous solution, 800 μL of the above monochromatic magnetic fluorescent microsphere aqueous solution and 90 μL tetraeth...

Embodiment 3

[0051] Preparation of QDs 1 Fluorescence intensity / QDs 2 Magnetic Fluorescent Encoded Microspheres with a Fluorescence Intensity of 2:5 Amino Functional Double Core-Shell Structure

[0052] Step a) the preparation of superparamagnetic iron ferric oxide nanocrystals is the same as step a) of Example 1;

[0053] Step b) The preparation of the CdTe quantum dots modified by mercaptosuccinic acid with high fluorescence intensity is the same as step b) of Example 1;

[0054] Step c) monochromatic magnetic fluorescent microspheres (Fe 3 o 4 -QDs 1 / SiO 2 ) with the step c) of embodiment 1;

[0055] Step d) Preparation of QDs 1 Fluorescence intensity / QDs 2 Magnetic fluorescently encoded microspheres with a fluorescence intensity of 2:5 amino-functionalized double core-shell structure: add 7.5ml cyclohexane, 1.77ml TritonX-100, 1.8ml n-hexanol, 400μL QDs in a 50ml Erlenmeyer flask 2 (0.0012 μmol) aqueous solution, 600 μL of the above-mentioned single-color magnetic fluorescent...

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Abstract

The invention belongs to the technical field of biomolecular labeling, in particular to a method for preparing an amino functional magnetic fluorescent coding microsphere with double-nucleocapsid structure. Two semiconductor quantum dots with different fluorescence colors and superparamagnetic ferric oxide nano-particles are embedded in a same silicon dioxide nano-particle in inverse microemulsion to form an amino functional magnetic fluorescent coding microsphere which has the granularity between 40nm and 100nm, high fluorescence intensity and high stability. The invention adjusts the fluorescence position and the intensity of the microsphere by changing the sort and the proportion of the quantum dots of different fluorescence colors and realizes different fluorescent coding. The addition of superparamagnetic ferroferric oxide ensures that the microsphere can gather and move fast in a targeted way under the action of an externally-applied magnetic field. Through amido modification on the surface of the microsphere, the invention can be widely applied to the fields of immunological test, nucleic acid hybridization, gene analysis, cell classification and imaging, etc.

Description

technical field [0001] The invention belongs to the technical field of biomolecular labeling, and in particular relates to a method for preparing amino-functionalized double-core-shell structure magnetic fluorescent coding microspheres. Background technique [0002] The labeling and detection of biomolecules has always been an important content in the field of bioanalysis. At present, the most commonly used fluorescent probes for protein research are organic fluorescent dyes. Although organic fluorescent dyes have a solid research foundation in bioconjugation, they are prone to photobleaching due to their narrow excitation spectrum and wide emission spectrum. Since the fluorescence of different dyes requires different excitation light sources, it is difficult to achieve simultaneous detection of multiple indicators. These defects limit their wide application to a certain extent; while semiconductor quantum dots (QDs) have color adjustable with size, a single light source It...

Claims

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Application Information

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IPC IPC(8): B01J13/02C09K11/88H01F1/11
Inventor 苏星光王冠男
Owner JILIN UNIV
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