Method for preparing blood vessel middle layer scaffold material used for biodegradable tissue engineering

A scaffold material and tissue engineering technology, which is applied in the field of preparing biodegradable vascular intermediate layer scaffold materials for tissue engineering, can solve the problems of less favorable cell adsorption and growth, cannot meet tissue engineering, and fast degradation rate, and can eliminate hidden dangers of viruses. , Excellent mechanical strength, low immune rejection effect

Active Publication Date: 2009-10-14
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although both collagen and silk fibroin have good biocompatibility, scaffold materials constructed solely with collagen have poor mechanical properties and a fast degradation rate; scaffold materials constructed solely with silk fibroin are not conducive to the adsorption and growth of cells , can not meet the requirements of tissue engineering

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Dissolve human-like collagen into a 1.5% solution with distilled water, and dissolve degummed silk fibroin in 40% CaCl under water bath conditions 2 In the solution, after dialysis, a silk fibroin solution with a concentration of 3% was obtained, and then the two were mixed according to a mass ratio of 2:1, and after stirring evenly, 40 μL of 25% glutaraldehyde aqueous solution was added, stirred gently for 30 minutes, and vacuum removed after filtration. Bubble, pour into a tubular mold, cross-link at 4°C for 2 days, freeze in a -80°C refrigerator for 5 hours to form, and vacuum-dry at -10°C to form a vascular inner layer stent. After washing with distilled water for 5 times, freeze-dry again to form. 60 Ready to use after disinfection.

Embodiment 2

[0024] Dissolve humanoid collagen into a 2% solution with distilled water, and dissolve degummed silk fibroin in 45% CaCl under water bath conditions 2 In the solution, a silk fibroin solution with a concentration of 3% was obtained after dialysis, and then the two were mixed according to a mass ratio of 5:1, and 0.4% glycerol of the total amount of the solution was added, and after stirring evenly, 40 μL of 25% glutaraldehyde was added Aqueous solution, gently stirred for 30 minutes, filtered, vacuum degassed, poured into a tubular mold, cross-linked at 4°C for 2 days, then placed in a -80°C refrigerator for 5 hours to form, and vacuum-dried at -10°C to make a vascular inner layer stent, washed with distilled water After 5 times, re-freeze-dried to shape, cobalt 60 Ready to use after disinfection.

Embodiment 3

[0026] Dissolve human-like collagen into a 1.5% solution with distilled water, and dissolve degummed silk fibroin in 40% CaCl under water bath conditions 2 In the solution, a silk fibroin solution with a concentration of 3% was obtained after dialysis, and then the two were mixed at a mass ratio of 6:1, and 0.4% of the total amount of the solution was added with 1.3-butanediol. After stirring evenly, 40 μL of 25% silk fibroin was added Glutaraldehyde aqueous solution, stirred gently for 30 minutes, filtered, vacuum degassed, poured into a tubular mold, cross-linked at 4°C for 2 days, placed in a -80°C refrigerator for 5 hours to form, and vacuum-dried at -10°C to make a vascular inner layer stent , washed with distilled water for 5 times, then re-freeze-dried to shape, cobalt 60 Ready to use after disinfection.

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Abstract

The invention discloses a method for preparing blood vessel middle layer scaffold material used for biodegradable tissue engineering, comprising the steps: human-like collagen is dissolved to be solution with the mass percent concentration of 1-3% by distilled water; degumming silk protein is dissolved in 30-50% of CaCl2 solution to be dialyzed to obtain the silk protein solution with the concentration of 2-4%; the two obtained solutions are evenly mixed together, mixed with glutaric dialdehyde water solution with the mass percent concentration of 20-50% and stirred; after being filtered, the obtained solution is processed by vacuum defoamation; the mixed solution is injected into a tubular shape mould to be processed by cross linkage at the temperature of 4 DEG C for 2 days, and then put into a refrigerator with the temperature of -80 DEG C to be frozen and dried, so that the blood vessel middle layer scaffold is prepared. Compared with the prior art, the prepared scaffold material has excellent mechanical capacity, biocompatibility, blood compatibility and immunity compatibility as well as lower immunity rejection reaction, thoroughly avoids the virus hidden trouble which is inevitable for animal collagen, and greatly improves the safety.

Description

technical field [0001] The invention relates to a method for preparing a vascular middle layer scaffold material for biodegradable tissue engineering, and belongs to the field of biomedical materials. Background technique [0002] The fabrication of vascular middle layer scaffold material is a very critical link in the field of vascular tissue engineering. As a scaffold for vascular smooth muscle cell implantation, it should not only have good biocompatibility and biodegradability, and a suitable pore structure, but also have certain strength and plasticity, and be able to resist blood flow pressure and shear force, so that inoculation After cell adhesion, proliferation, and differentiation, they are distributed and arranged in an orderly manner in the scaffold space, synthesize appropriate extracellular matrix (ECM), and differentiate into a blood vessel-like three-dimensional structure tissue with specific functions of the vascular middle layer. [0003] Collagen is the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/56A61L27/58A61L27/22A61L27/24
Inventor 范代娣朱晨辉马晓轩米钰骆艳娥马沛惠俊峰朱晓丽李万华梁丽华陈岚
Owner NORTHWEST UNIV(CN)
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