Glucose sensor electrode without enzyme with nickel-aluminium-hydrotalcite nano-chip arrays on substrate of titanium

A glucose sensor and nanosheet array technology, applied in the field of enzyme-free glucose sensor electrodes, can solve the problems of difficult storage, poor electrochemical performance, and expensive glucose oxidase, and achieve the effect of easy storage and high sensitivity

Inactive Publication Date: 2009-12-09
HUAZHONG NORMAL UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Because glucose oxidase is expensive and difficult to store, it must be refrigerated at low temperature and easily inactivated. If it is not stored properly, glucose oxidase will lose its catalytic activity and lose the function of glucose concentration detection. Therefore, this electrode is cheap, convenient and widely used. Just exist the difficulty that is difficult to overcome; Simultaneously, th

Method used

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  • Glucose sensor electrode without enzyme with nickel-aluminium-hydrotalcite nano-chip arrays on substrate of titanium
  • Glucose sensor electrode without enzyme with nickel-aluminium-hydrotalcite nano-chip arrays on substrate of titanium
  • Glucose sensor electrode without enzyme with nickel-aluminium-hydrotalcite nano-chip arrays on substrate of titanium

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Embodiment 1

[0027] Nickel-aluminum hydrotalcite nanosheet array electrode preparation: 2.036 grams of nickel nitrate hexahydrate (Ni(NO 3 ) 2 ·6H 2 O), 1.313 grams of aluminum nitrate nonahydrate (Al(NO 3 ) 3 .9H 2 O), 4.200 grams of urea (CON 2 h 4 ) was dissolved in 70.0 ml of water, stirred with a magnetic stirrer to fully dissolve, mixed evenly, and made into a mixed solution; a titanium metal sheet with a thickness of 0.2 mm, a width of 2.0 mm, and a length of 60.0 mm was washed with dilute hydrofluoric acid (HF) After soaking in the solution and rinsing with clean water, put it into the prepared solution; then place the solution together with the titanium metal in the polytetrafluoroethylene liner in the autoclave and heat it to 95°C for 36 hours; after natural cooling, place the The titanium metal sheet is taken out to obtain an enzyme-free glucose sensor electrode sample of the nickel-aluminum hydrotalcite nanosheet array on the titanium substrate. The samples were subjecte...

Embodiment 2

[0029] Nickel-aluminum hydrotalcite nanosheet array electrode preparation: 2.036 grams of nickel nitrate hexahydrate (Ni(NO 3 ) 2 ·6H 2 O), 1.313 grams of aluminum nitrate nonahydrate (Al(NO 3 ) 3 .9H 2 O), 4.200 grams of urea (CON 2 h 4) was dissolved in 140 ml of water, stirred with a magnetic stirrer to fully dissolve, mixed evenly, and made into a mixed solution; a titanium metal sheet with a thickness of 0.2 mm, a width of 2.0 mm, and a length of 60.0 mm was washed with dilute hydrofluoric acid (HF) After soaking in the solution and rinsing with clean water, put it into the prepared solution; then put the aqueous solution together with the titanium metal in the polytetrafluoroethylene liner in the autoclave and heat it to 95°C for 72 hours; after natural cooling, place the The titanium metal sheet is taken out to obtain an enzyme-free glucose sensor electrode sample of the nickel-aluminum hydrotalcite nanosheet array on the titanium substrate. The samples were subj...

Embodiment 3

[0031] Nickel-aluminum hydrotalcite nanosheet array electrode preparation: 2.036 grams of nickel nitrate hexahydrate (Ni(NO 3 ) 2 ·6H 2 O), 1.313 grams of aluminum nitrate nonahydrate (Al(NO 3 ) 3 .9H 2 O), 4.200 grams of urea (CON 2 h 4 ) was dissolved in 70.0 ml of water, stirred with a magnetic stirrer to fully dissolve, mixed evenly, and made into a mixed solution; a titanium metal sheet with a thickness of 0.2 mm, a width of 2.0 mm, and a length of 60.0 mm was washed with dilute hydrofluoric acid (HF) After soaking in the solution and rinsing with clean water, put it into the prepared solution; then put the aqueous solution together with the titanium metal in the polytetrafluoroethylene liner in the autoclave and heat it to 75°C for 72 hours; after natural cooling, place the The titanium metal sheet is taken out to obtain an enzyme-free glucose sensor electrode sample of the nickel-aluminum hydrotalcite nanosheet array on the titanium substrate. The samples were su...

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Abstract

A glucose sensor electrode without enzyme with nickel-aluminium-hydrotalcite nano-chip arrays on a substrate of titanium is composed of the nickel-aluminium-hydrotalcite nano-chip arrays on the substrate of the titanium, the thickness of the single nickel- aluminium-hydrotalcite nano-chip is 20-40nm, and the diameter thereof is 200-400nm; the nickel-aluminium-hydrotalcite nano-chip arrays are distributed on the surface of the titanium metal vertically, evenly and densely in an arrayed manner. The preparation method is as follows: the titanium metal is soaked in hydrofluoric acid solution, and is placed into aqueous solution prepared by nickel nitrate hexahydrate, aluminum nitrate nonahydrate and carbamide after being washed by clean water; and then the titanium metal is sealed in a polytetrafluoroethylene liner in an autoclave and heated to over 70 DEG C for more than 36 hours; the titanium metal is taken out after being cooled naturally to obtain an electrode sample, wherein, the concentration of the nickel nitrate hexahydrate is 2.036g/70-140ml of water, the concentration of the aluminum nitrate nonahydrate is 1.313g/70-140ml of water and the concentration of the carbamide is 4.200g/70-140ml of water. The electrode is applicable to the products in the fields such as biology, medicine, electronic instruments and the like. The glucose sensor electrode has high sensitivity to glucose detection, low detection limit, fast response, large linear range, stable work performance.

Description

technical field [0001] The invention relates to an enzyme-free glucose sensor electrode, in particular to an enzyme-free glucose sensor electrode on a nickel-aluminum hydrotalcite nanosheet array on a titanium substrate. It belongs to the fields of biology, medicine, analytical chemistry, electronic equipment, electronic information, etc., and is mainly used in electrochemical analysis instruments of biology, clinical medicine, chemistry, chemical industry and other products. Background technique [0002] On the ordinary electrochemical glucose detection electrode, it is necessary to use chitosan or Nafion to immobilize the glucose oxidase that plays an electrochemical reaction on the electrode. Because glucose oxidase is expensive and difficult to store, it must be refrigerated at low temperature and easily inactivated. If it is not stored properly, glucose oxidase will lose its catalytic activity and lose the function of glucose concentration detection. Therefore, this ele...

Claims

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Application Information

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IPC IPC(8): G01N27/30
Inventor 黄新堂李新艾汉华刘金平
Owner HUAZHONG NORMAL UNIV
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