Glucose sensor electrode without enzyme with nickel-aluminium-hydrotalcite nano-chip arrays on substrate of titanium

Abstract

A glucose sensor electrode without enzyme with nickel-aluminium-hydrotalcite nano-chip arrays on a substrate of titanium is composed of the nickel-aluminium-hydrotalcite nano-chip arrays on the substrate of the titanium, the thickness of the single nickel- aluminium-hydrotalcite nano-chip is 20-40nm, and the diameter thereof is 200-400nm; the nickel-aluminium-hydrotalcite nano-chip arrays are distributed on the surface of the titanium metal vertically, evenly and densely in an arrayed manner. The preparation method is as follows: the titanium metal is soaked in hydrofluoric acid solution, and is placed into aqueous solution prepared by nickel nitrate hexahydrate, aluminum nitrate nonahydrate and carbamide after being washed by clean water; and then the titanium metal is sealed in a polytetrafluoroethylene liner in an autoclave and heated to over 70 DEG C for more than 36 hours; the titanium metal is taken out after being cooled naturally to obtain an electrode sample, wherein, the concentration of the nickel nitrate hexahydrate is 2.036g / 70-140ml of water, the concentration of the aluminum nitrate nonahydrate is 1.313g / 70-140ml of water and the concentration of the carbamide is 4.200g / 70-140ml of water. The electrode is applicable to the products in the fields such as biology, medicine, electronic instruments and the like. The glucose sensor electrode has high sensitivity to glucose detection, low detection limit, fast response, large linear range, stable work performance.

Description

technical field [0001] The invention relates to an enzyme-free glucose sensor electrode, in particular to an enzyme-free glucose sensor electrode on a nickel-aluminum hydrotalcite nanosheet array on a titanium substrate. It belongs to the fields of biology, medicine, analytical chemistry, electronic equipment, electronic information, etc., and is mainly used in electrochemical analysis instruments of biology, clinical medicine, chemistry, chemical industry and other products. Background technique [0002] On the ordinary electrochemical glucose detection electrode, it is necessary to use chitosan or Nafion to immobilize the glucose oxidase that plays an electrochemical reaction on the electrode. Because glucose oxidase is expensive and difficult to store, it must be refrigerated at low temperature and easily inactivated. If it is not stored properly, glucose oxidase will lose its catalytic activity and lose the function of glucose concentration detection. Therefore, this ele...

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Problems solved by technology

Because glucose oxidase is expensive and difficult to store, it must be refrigerated at low temperature and easily inactivated. If it is not stored properly, glucose oxidase will lose its catalytic activity and lose the function of glucose concentration detection. Therefore, this electrode is cheap, convenient and widely used. Just exist the difficulty that is difficult to overcome; Simultaneously, the chitosan of immobilizing glucose oxidase or Nafion all has the property of being slightly soluble in water, so, the glucose oxidase that plays electrochemical reaction will slow down along with chitosan or Nafion. Slowly soluble in water, that is, long-term or multiple use, the glucose oxidase on the electrode will become less and less, and its electrochemical performance will become worse and worse

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