Preparation method and application of clove leaf total iridoid glycoside

A technology for total iridoid glycosides and cloves, which is applied in the field of preparation of total iridoid glycosides extracts in clove leaves, can solve problems such as difficulty in getting rid of inherent shortcomings, excessive dosage, complex components, etc., and achieves low cost , the effect of high desorption rate and large adsorption capacity

Inactive Publication Date: 2009-12-23
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] Due to the complex composition of the total extract of clove leaves, it is difficult for the finished preparation to get rid of the inherent shortcomings of traditional Chinese medicine dosage forms of thick, large, and black, and the total extract contains more impurities such as sugars, which makes the raw material medicine have greater hygroscopicity. Lead to excessive doses, and also limit the choice of dosage forms

Method used

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  • Preparation method and application of clove leaf total iridoid glycoside
  • Preparation method and application of clove leaf total iridoid glycoside
  • Preparation method and application of clove leaf total iridoid glycoside

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Embodiment 1

[0020] The preparation of embodiment 1 clove leaves total iridoid glycosides

[0021] see figure 1 , crush 1 kg of clove leaves to 10-20 meshes, add 12 times volume percentage of ethanol with a concentration of 30%, extract by percolation, collect the effluent after soaking for one week for the first time, collect the effluent after soaking for three days for the second time, filter, and combine The extract was concentrated to 0.5g crude drug / ml, added hydrochloric acid to adjust the pH value to 4, and passed through the D-101 macroporous adsorption resin chromatography column with a flow rate of 2BV / h, and the ratio of the loading sample solution to the dry resin was 1:5, the diameter-to-height ratio is 1:6, first wash the resin with 4BV of water, the elution flow rate is 2BV / h, discard the water eluent; then elute with 50% ethanol by volume, and elute The flow rate is 2BV / h, the eluent is depressurized to recover ethanol, concentrated to a relative density of 1.15 (measured...

Embodiment 2

[0022] The preparation of embodiment 2 clove leaves total iridoid glycosides

[0023] see figure 1 , in 2kg clove leaves crushed to 10-20 mesh, add 15 times the amount of water, heat and reflux extraction 3 times, 2h each time, filter, combine the extracts, concentrate to a concentration of 1.0g crude drug / ml, add hydrochloric acid after filtration Adjust the pH value to 4, and pass through the DM-301 macroporous adsorption resin chromatography column at a flow rate of 3BV / h. The ratio of the loading liquid to the dry resin is 1:8, and the ratio of diameter to height is 1:10. Wash the resin with 6BV of water, the elution flow rate is 4BV / h, discard the water eluent; then elute with methanol with a volume percentage concentration of 80%, the elution flow rate is 3BV / h, and the eluent is decompressed to recover methanol , concentrated to a relative density of 1.25 (measured at 50°C), and freeze-dried to obtain total iridoid glycosides of clove leaves. The measured total iridoi...

Embodiment 3

[0024] The preparation of embodiment 3 clove leaves total iridoid glycosides

[0025] see figure 1 , clove leaves 5kg moderately pulverized, add 8 times the amount of 10% ethanol, ultrasonically extract 3 times, 0.5h each time, filter, combine the extracts, recover ethanol under reduced pressure, add water to adjust the concentration to 1.5g crude drug / ml, add hydrochloric acid to adjust When the pH value reaches 4, it passes through the NKA-9 macroporous adsorption resin chromatography column. The ratio of the loading liquid to the dry resin is 1:9 according to the amount of solid matter and the ratio of diameter to height is 1:8. The resin is first washed with 4BV of water. The elution flow rate is 2BV / h, discard the water eluent; then elute with ethanol with a volume percentage concentration of 70%, the elution flow rate is 3BV / h, and the eluent is decompressed to recover ethanol, and concentrated to a relative density of 1.15 (measured at 50°C), and spray-dried to obtain ...

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Abstract

The invention provides a preparation method of a clove leaf total iridoid glycoside extract. The preparation method comprises the following steps: clove leaf medicinal materials are pulverized and added with water or a water/alcohol solvent system, heated circumfluence/diacolation or ultrasonic extraction, filtration and centrifugation are adopted, the pH value is regulated to 2-5, an extracting solution passes through a big-hole adsorptive resin chromatographic column, the water and an alcohol eluent are used for washing resin, the eluent is subject to pressure reduction to recover alcohol, and freezing or spray drying is carried out to obtain the total iridoid glycoside. The transfer rate of the clove leaf total iridoid glycoside prepared by using the method can reach more than 75%, wherein the content of syringopicroside reaches 53.42%. Medicines used for treating infective diseases, such as upper respiratory tract infection, acute or chronic tonsillitis, acute enteritis, bacillary dysentery and the like by adding various medicinal auxiliary materials, and the medicament form of the prepared medicines is solid preparations.

Description

technical field [0001] The invention belongs to the field of plant and traditional Chinese medicine extracts, and in particular relates to a preparation method and application of total iridoid glycosides extract from clove leaves. Background technique [0002] Inflammation is the source of all diseases and is a common clinical pathological process, which can occur in various parts, tissues and organs of the body. Because inflammation is a high-incidence and universal disease, it is mainly treated with antibacterial and anti-inflammatory methods in clinic. According to data, the use of antibiotics in developed countries in Europe and the United States accounts for about 10% of all drugs, while in my country, the tertiary hospitals account for 30%, and the grassroots hospitals may be as high as 50%. It can be seen that the frequency of antibiotic use in my country is very high. (http: / / drug.sosyao.com). In the development of antibiotics for more than 60 years, the first 30 ye...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/61A61P11/00A61P11/04A61P1/16A61P1/00A61P1/12A61P1/04A61P31/04A61K127/00
CPCY02A50/30
Inventor 刘欣周长新
Owner ZHEJIANG UNIV
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