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Zinc-rich yeast with high biomass, breeding selection method and application thereof

A high-biomass, zinc-rich yeast technology, applied in microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the large difference in zinc ion absorption and transformation ability, unfavorable industrial production, and zinc resistance. problem, to achieve the effect of broad practical application prospects, strong practicability, and simple methods

Inactive Publication Date: 2010-01-20
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, these products have obvious defects and deficiencies: the research on zinc-rich yeast at home and abroad mainly focuses on the transfer and regulation of zinc ions by yeast cells or the induction of nitrosoguanidine (NTG), cobalt 60, ultraviolet rays, etc. The main defect is that different yeast strains have large differences in resistance to zinc, and thus have large differences in the ability to absorb and transform zinc ions; moreover, the zinc content in yeast cells is low, and the product lacks stability. Not conducive to industrial production, see Kong Lin et al., Journal of Food and Biotechnology, Volume 25, Issue 6, November 2006

Method used

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  • Zinc-rich yeast with high biomass, breeding selection method and application thereof

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Embodiment 1

[0016] Example 1, Breeding of high biomass zinc-rich yeast (Saccharomyces cerevisiae) F-090428X

[0017] The breeding of high biomass zinc-rich yeast (Saccharomyces cerevisiae) (F-090428X) is divided into the following steps:

[0018] 1) According to the determination of the tolerance of yeast to zinc sulfate, the initial screening was carried out, and the tolerance of brewer's yeast on the wort solid medium containing 5000 μg / ml, 6000 μg / ml, 8000 μg / ml, and 10000 μg / ml of gradient zinc ion concentration was measured. For the growth situation, 2 strains were selected which grew well on the wort solid medium containing 10000 μg / ml zinc ions.

[0019] 2) Determination of the cell biomass and cell zinc content of a strain obtained from the primary screening cultured in wort liquid medium containing 10000 μg / ml zinc ions. Through domestication and screening, a strain (F-090415) with a dry cell zinc content of 35.5 mg / g and a dry cell weight of 9.2 g / L was obtained.

[0020] 3) I...

Embodiment 2

[0022] Example 2, Optimal culture conditions of high biomass zinc-rich yeast (Saccharomyces cerevisiae) F-090428X

[0023] 1) The culture conditions of high-biomass zinc-rich yeast (Saccharomyces cerevisiae) F-090428X (including medium components, zinc salt concentration, medium pH value, aeration, Inoculum size and fermentation time, etc.) experiment, by culture condition experiment, determined optimal culture condition: wort+0.3% urea culture medium, total sugar content is 5%-20%, zinc salt (such as zinc sulfate) concentration in the culture medium 10000-15000 μg / ml, the pH of the culture medium is 4.5-5.5, the inoculum size is 10%-30%, and the culture is stirred at 28°C-30°C for 16-48h.

[0024] 2) Fermentation and cultivation of zinc-rich yeast with high biomass and high zinc content under optimized fermentation conditions: under optimized conditions, the dry cell weight per liter of culture medium can reach more than 12g, and the zinc content per gram of dry cells can rea...

Embodiment 3

[0025] Embodiment 3, the production of high biomass zinc-rich yeast

[0026] The production process of zinc-rich yeast products with high biomass and high zinc content includes:

[0027] Inclined surface bacteria → first-level shake flask strain cultivation → second-level shake flask seed cultivation → seed tank → second-level liquid seed tank → fermentation tank → storage tank → solid-liquid separation → freeze-drying or spray drying → (or use plate and frame pressing Or centrifuge to collect zinc-enriched yeast cells → pulverize) → zinc-enriched with high biomass and high zinc-enriched content → sub-package → preparation (feed additive, or capsule, tablet, etc.) → packaging.

[0028] Below the production process is further described as follows:

[0029] (1) Bacteria culture on inclined plane

[0030] High-biomass zinc-rich yeast (Saccharomyces cerevisiae) F-090428X was inoculated on the wort solid slant with a total sugar content of 16% and a zinc salt (such as zinc sulfate)...

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Abstract

The invention relates to a zinc-rich yeast with high biomass, a breeding selection method and application thereof. A zinc-rich yeast bacterial strain with high biomass is Sacharomyces cerevisiae) F-090428X. The bacterial strain is preserved in China General Microbiological Culture Collection Center (CGMCC for short) in July 2nd, 2009 with the preservation number of No.3156. The breeding selection method of the zinc-rich yeast with high biomass adopts microwave irradiation. The zinc-rich yeast with high biomass can be used for preparing high zincic human food or high zincic additives or high zincic medicaments, or can be used for preparing high zincic animal feed or high zincic feed addictive.

Description

technical field [0001] The invention relates to a variety of microorganisms, a breeding method for the microorganisms and its application. Specifically, the microorganisms involved in the invention are high-biomass zinc-rich yeasts. Background technique [0002] In recent years, some products called "zinc-rich yeast" have come out. For example, Li Wenzhen and others used beer yeast (Sacchsromyces cerevisiae) as the starting strain, and used corn saccharification to add appropriate nutrients and zinc ions, and obtained it through fermentation experiments. Products, Journal of Microbiology, Volume 14, Issue 1, 1994; Hu Zhenying et al. made 50 generations of adaptive culture of beer yeast strains under high zinc conditions, and obtained zinc-rich yeast strains, Journal of Chinese Veterinary Medicine, Issue 6, 1997; Chen Peilin : Take the yeast IFO2346 strain as the strain, add an appropriate amount of zinc ions in the medium, and obtain high-zinc yeast products through fermenta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18C12N15/01C12N13/00A23L1/28A23L1/304A23K1/00A23K1/16A61K36/064A61P3/02C12R1/865A23K10/18A23K20/20A23L31/10A23L33/16
Inventor 胡振英程富胜辛蕊华罗永江罗超应郑继芳李建喜
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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