Method for preparing magnetic microbe immobilizing material for soil pollution repair and application thereof

A magnetic microorganism and soil pollution technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as secondary pollution, difficulty in controlling the particle size of immobilized materials, and adverse effects of immobilized microorganism growth , to achieve the effect of strong adsorption capacity

Inactive Publication Date: 2010-01-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the above-mentioned immobilized materials are difficult to separate and recycle after being applied to the soil, which is likely to cause waste of resources and potential secondary pollution
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Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0014] Example 1

[0015] 1) After air drying, the waste rice straw materials are crushed and passed through a 60-mesh sieve;

[0016] 2) The crushed straw particles are added to the 0.1mol / L iron salt solution, and 4mol / L NaOH solution is added dropwise to the solution pH 9-10 under magnetic stirring. The solid precipitate is separated by filtration, dried, and carbonized with a lower limit of oxygen at 300°C for 2 hours to obtain a carrier material;

[0017] 3) Connect the white-rot fungus suspension at a solid-liquid ratio of 2mL / g, and add sodium alginate (1-2g sodium alginate / 100mL water) and stir well;

[0018] 4) Add the above mixed suspension dropwise to 2% CaCl by mass 2 The solution is made into particles (1-20mm in diameter) and proliferated for 12 hours to obtain magnetically immobilized microbial active particles MIM-1.

[0019] 5) Add the prepared microbial immobilized particles to the soil contaminated by polycyclic aromatic hydrocarbons at a rate of 10 tons per hectare ...

Example Embodiment

[0021] Example 2

[0022] 1) The sawdust waste after air drying is crushed and passed through a 20-mesh sieve;

[0023] 2) The crushed sawdust particles are added to the 0.1 mol / L iron salt solution, and the 2 mol / L NaOH solution is added dropwise to the solution pH 9-10 under magnetic stirring. The solid precipitate is separated by filtration, dried, and carbonized with the lower limit oxygen at 400°C for 1 hour to prepare the carrier material MIM-2;

[0024] 3) Connect the white rot fungus suspension at a solid-liquid ratio of 1mL / G, and add sodium alginate (1-2g sodium alginate / 100mL water) and stir well;

[0025] 4) Add the above mixed suspension dropwise to 2% CaCl by mass 2 The solution is made into particles (1-20mm in diameter) and proliferated for 24 hours to obtain magnetically immobilized microbial active particles.

[0026] 5) Add the prepared microbial immobilized particles to the soil contaminated by polycyclic aromatic hydrocarbons at a rate of 20 tons per hectare of soi...

Example Embodiment

[0028] Example 3

[0029] 1) After air drying, the waste peels are crushed and passed through a 100-mesh sieve;

[0030] 2) The crushed straw particles are added to the 0.1mol / L iron salt solution, and 3mol / L NaOH solution is added dropwise to the solution pH 9-10 under magnetic stirring. The solid precipitate is separated by filtration, dried, and carbonized with a lower limit oxygen at 250°C for 3 hours to obtain a carrier material;

[0031] 3) Connect the mixed suspension of white rot fungi and bacteria according to the solid-liquid ratio of 5mL / g, and add sodium alginate (1-2g sodium alginate / 100mL water) and stir well;

[0032] 4) Add the above mixed suspension dropwise to 3% CaCl by mass 2 The solution is made into particles (1-20mm in diameter) and proliferated for 12 hours to obtain magnetically immobilized microbial active particles MIM-3.

[0033] 5) Add magnetically immobilized microbial active particles according to a 2% dosage ratio, and try to mix them thoroughly with the...

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PUM

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Abstract

The invention discloses a method for preparing a magnetic microbe immobilizing material for soil pollution repair and an application thereof. The method comprises the following steps: (1) drying, pulverizing and sieving waste biomass in 20-100 meshes; (2) charging the sieved biomass into 0.1 mol/L of iron salt solution, dropwise adding 3-6 mol/L of NaOH solution during stirring till the pH value is 9 to 10; filtering, drying and carbonizing sediments with limited oxygen at 100-400 DEG C for 1 hour to 4 hours to prepare a carrier material; (3) injecting bacterial suspension according to the solid-to-liquid ratio of 1-10mL/g and adding sodium alginate; (4) dropwise adding the mixed suspension into 2-5 percent of CaCl2 solution to prepare a particle with the diameter of 1 mm to 2 mm; breeding the particle to prepare a magnetic microbe immobilizing material; and (5) charging the prepared material into polluted soil according to 2-20 ton/hectare and plowing 0 cm to 30 cm of soil in the surface layer, after 3 to 12 months, recovering by magnetic separation, activating and recycling. The invention has the advantages of magnetic material and controllable particle parameter, can be recovered by magnetic separation after use and is suitable for in-situ biological repair and heterotopic slurry reaction vessel repair of the polluted soil.

Description

technical field [0001] The invention relates to a preparation method and application of a magnetic microorganism immobilization material for remediation of polluted soil. Background technique [0002] Soil has always been an indispensable natural resource for human survival. However, with the continuous development of modern industrial and agricultural production, the extensive use of agricultural chemical fertilizers and pesticides, and the continuous invasion of industrial wastewater, urban domestic sewage and solid waste, soil pollution has become increasingly serious, threatening human health and ecological security. Compared with heavy metal pollution, soil persistent organic pollutant pollution, such as polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs), has become the focus of attention in my country's soil pollution control due to its high risk. [0003] Soil microbial remediation is the main way to remove organic pollutants in the soil env...

Claims

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Application Information

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IPC IPC(8): C12N11/04B09C1/10C12R1/07C12R1/38C12R1/645C12R1/785C12R1/66C12R1/80
Inventor 陈宝梁陈再明王银善
Owner ZHEJIANG UNIV
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