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Method for preparing magnetic microbe immobilizing material for soil pollution repair and application thereof

A magnetic microorganism and soil pollution technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as secondary pollution, difficulty in controlling the particle size of immobilized materials, and adverse effects of immobilized microorganism growth , to achieve the effect of strong adsorption capacity

Inactive Publication Date: 2010-01-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the above-mentioned immobilized materials are difficult to separate and recycle after being applied to the soil, which is likely to cause waste of resources and potential secondary pollution
Moreover, in the preparation process, the particle size of these immobilized materials is difficult to control
Too large or too small particle size will have adverse effects on the growth of immobilized microorganisms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1) The waste rice straw raw material after air drying is crushed and passed through a 60-mesh sieve;

[0016] 2) Add the crushed straw particles into 0.1mol / L iron salt solution, and add 4mol / L NaOH solution dropwise under magnetic stirring until the pH of the solution is 9-10. The solid precipitate was separated by filtration, dried, and carbonized with limited oxygen at 300°C for 2 hours to obtain the carrier material;

[0017] 3) Add the white rot fungus suspension at a solid-to-liquid ratio of 2mL / g, add sodium alginate (1-2g sodium alginate / 100mL water) and stir evenly;

[0018] 4) Adding the above mixed suspension dropwise to 2% CaCl by mass percentage 2 Granules (with a diameter of 1-20 mm) are prepared in the solution, and the multiplication culture is carried out for 12 hours to obtain the magnetically immobilized microbial active particle MIM-1.

[0019] 5) Add the prepared microbe-immobilized particles into polycyclic aromatic hydrocarbon-contaminated soil ...

Embodiment 2

[0022] 1) Sawdust waste after air drying is pulverized through a 20-mesh sieve;

[0023] 2) Add the pulverized sawdust particles into 0.1mol / L iron salt solution, and add 2mol / L NaOH solution dropwise under magnetic stirring until the pH of the solution is 9-10. The solid precipitate was separated by filtration, dried, and carbonized with limited oxygen at 400°C for 1 hour to obtain the carrier material MIM-2;

[0024] 3) Add the white rot fungus suspension at a solid-to-liquid ratio of 1mL / G, add sodium alginate (1-2g sodium alginate / 100mL water) and stir evenly;

[0025] 4) Adding the above mixed suspension dropwise to 2% CaCl by mass percentage 2 Granules (with a diameter of 1-20 mm) are made in the solution, and the magnetically immobilized microbial active particles can be obtained by proliferating and culturing for 24 hours.

[0026] 5) Add the prepared microbe-immobilized particles into polycyclic aromatic hydrocarbon-contaminated soil at a rate of 20 tons per hectare...

Embodiment 3

[0029] 1) After air-drying, the discarded peel waste is crushed through a 100-mesh sieve;

[0030] 2) Add the crushed straw particles into 0.1mol / L iron salt solution, and add 3mol / L NaOH solution dropwise under magnetic stirring until the pH of the solution is 9-10. The solid precipitate was separated by filtration, dried, and carbonized with limited oxygen at 250°C for 3 hours to obtain the carrier material;

[0031] 3) Insert the mixed suspension of white rot fungi and bacteria at a solid-to-liquid ratio of 5mL / g, and add sodium alginate (1-2g sodium alginate / 100mL water) and stir evenly;

[0032] 4) Adding the above mixed suspension dropwise to 3% CaCl by mass percentage 2 Granules (with a diameter of 1-20 mm) are prepared in the solution, and the multiplication and cultivation are carried out for 12 hours to obtain magnetically immobilized microbial active particles MIM-3.

[0033] 5) Dosing magnetically immobilized microbial active particles according to a 2% dosage ra...

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Abstract

The invention discloses a method for preparing a magnetic microbe immobilizing material for soil pollution repair and an application thereof. The method comprises the following steps: (1) drying, pulverizing and sieving waste biomass in 20-100 meshes; (2) charging the sieved biomass into 0.1 mol / L of iron salt solution, dropwise adding 3-6 mol / L of NaOH solution during stirring till the pH value is 9 to 10; filtering, drying and carbonizing sediments with limited oxygen at 100-400 DEG C for 1 hour to 4 hours to prepare a carrier material; (3) injecting bacterial suspension according to the solid-to-liquid ratio of 1-10mL / g and adding sodium alginate; (4) dropwise adding the mixed suspension into 2-5 percent of CaCl2 solution to prepare a particle with the diameter of 1 mm to 2 mm; breeding the particle to prepare a magnetic microbe immobilizing material; and (5) charging the prepared material into polluted soil according to 2-20 ton / hectare and plowing 0 cm to 30 cm of soil in the surface layer, after 3 to 12 months, recovering by magnetic separation, activating and recycling. The invention has the advantages of magnetic material and controllable particle parameter, can be recovered by magnetic separation after use and is suitable for in-situ biological repair and heterotopic slurry reaction vessel repair of the polluted soil.

Description

technical field [0001] The invention relates to a preparation method and application of a magnetic microorganism immobilization material for remediation of polluted soil. Background technique [0002] Soil has always been an indispensable natural resource for human survival. However, with the continuous development of modern industrial and agricultural production, the extensive use of agricultural chemical fertilizers and pesticides, and the continuous invasion of industrial wastewater, urban domestic sewage and solid waste, soil pollution has become increasingly serious, threatening human health and ecological security. Compared with heavy metal pollution, soil persistent organic pollutant pollution, such as polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs), has become the focus of attention in my country's soil pollution control due to its high risk. [0003] Soil microbial remediation is the main way to remove organic pollutants in the soil env...

Claims

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Application Information

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IPC IPC(8): C12N11/04B09C1/10C12R1/07C12R1/38C12R1/645C12R1/785C12R1/66C12R1/80
Inventor 陈宝梁陈再明王银善
Owner ZHEJIANG UNIV
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