Tissue engineering scaffold containing alginate, glycosaminoglycan and collagen and preparation method thereof

Abstract

The invention belongs to the tissue engineering scaffold field and particularly provides a tissue engineering scaffold containing alginate, glycosaminoglycan and collagen and a preparation method thereof. The tissue engineering scaffold provided by the invention is prepared by the following raw materials by weight percent: 5-30% of chitosan, 5-30% of collagen, 5-30% of alginate, 0.1-3% of hyaluronic acid, 0.01-0.05% of crosslinking agent, 0.1-3% of chondroitin sulfate, 5-20% of polyvinyl alcohol and the balance tertiary distilled water. For optimization, the tissue engineering scaffold also contains a proper amount of cell growth factor, fibronectin and laminin. The tissue engineering scaffold can be processed and shaped and can be used to prepare products with different forms and thickness, the shaping method is easy to operate, the raw materials are easy to get and not expensive and the tissue engineering scaffold can be used for scale industrialized production.

Description

technical field [0001] The invention relates to a tissue engineering scaffold material, in particular to a tissue engineering scaffold containing alginate, glycosaminoglycan and collagen and a preparation method thereof. Background technique [0002] The defect or loss of function of tissues and organs due to accidents, diseases, genetics, and aging is the primary problem affecting human life and quality of life. When a tissue or organ in a certain part of the human body is traumatized, the clinically available treatment methods are autologous transplantation, allogeneic transplantation or xenotransplantation. However, these methods have shortcomings such as insufficient donors, immune rejection, and high surgical costs. A large number of patients died due to the lack of suitable donors and effective organ replacements, and were not treated. Therefore, how to realize the repair and regeneration of damaged tissues and organs, and how to realize the recovery of their functio...

AI Technical Summary

Problems solved by technology

[0012] At present, the tissue engineering scaffolds prepared by research at home and abroad generally have a single component distribution ratio, a small application range, poor controllability of degradation and absorption rates, and cannot form a three-dimensional through-hole porous structure in the scaffold, and cannot adjust the pore size according to the corresponding cell type. The size, volume and density cannot fully meet the comprehensive performance requirements of tissue engineering scaffolds. It is difficult to be suitable for the growth and reproduction of various tissue and organ cells, and it cannot be processed and shaped to prepare products of different shapes and thicknesses.