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Method for culturing tetraploid common head cabbage

A technology of head cabbage and its cultivation method, which is applied in the field of cultivation of tetraploid head cabbage, and can solve the problems of not being able to meet the requirements of raw food and insufficient brittleness of bulb leaves, etc.

Inactive Publication Date: 2010-02-17
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the problems in the prior art that the leaves of head cabbage varieties are not crisp enough to meet the requirements of raw food, etc., the invention provides a method for cultivating tetraploid head cabbage, and selects and breeds a batch of tetraploid head cabbage variety materials

Method used

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Experimental program
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Embodiment 1

[0011] Cytoplasmic male sterile line Anand CMS (well known and public. T. Cardi E D Earle, Production of new CMS Brassica oleraca by transfer of'Anand'cytoplasm from B.rapa through protoplastfusion. Theory Application Genetics, (1997) 94: 204-212) ) and the corresponding maintainer line Jinzaosheng (publicly known and public. Fang Zhiyuan, Sun Peitian, Liu Yumei, Yang Limei. Breeding of a new early-maturing spring cabbage variety 'Zhonggan No. 11' in northern China, Chinese vegetables, (1987) 4: 1~4; , Xu Helin, Ding Wanxia, ​​Wang Aimin. Breeding of a new variety of early-maturing spring cabbage "Chunfeng" in southern China, Chinese Vegetables, (1991) 4: 5-7) as material, using the chemical agent colchicine to induce tetraploid production . The concentration of the colchicine solution used is 0.15% (g / ml, the same below), and the diploid cabbage cytoplasmic male sterile line and the corresponding maintainer line (2n=2x=18) are injected with a microinjector at the cotyledon st...

Embodiment 2

[0013] Take yellow seedling varieties (publicly known and public. Yin Yuhua, Zhu Zongyuan, Hybrid first-generation autumn cabbage——Zhefeng No. 1, Agricultural Science and Technology Communication, 1989, 6:45; Yan Huiling, Agrobacterium-mediated genetic transformation system of cabbage, Northwest Agriculture and Forestry Master's thesis of University of Science and Technology, 2003) using the chemical agent colchicine to induce tetraploid production. The used colchicine solution concentration is 0.15% (g / ml, hereinafter the same), drop 2 drops of colchicine on the shoot tip growth point of the golden early diploid plant (2n=2x=18) with a microinjector at the cotyledon stage Vegetable solution, twice a day, once at 8:00 am to 9:00 pm, once at 2:00 pm to 3:00 pm, and treated continuously for 5 days. According to the number test, the first-generation tetraploid head cabbage golden early-growing mutant strain was obtained, and the chromosome number of the somatic cells of the mutan...

Embodiment 3

[0015] With the local variety chicken heart (public production. Huang Zhenyi, Xu Helin, Ding Wanxia, ​​Wang Aimin. Breeding of a new early-maturing spring cabbage variety "Chunfeng" in southern China, Chinese Vegetables, (1991) 4: 5-7) was induced by the chemical agent colchicine. Mutant tetraploid production. The colchicine solution concentration used is 0.15% (g / ml, the same below), and at the cotyledon stage, use a microinjector to drop 2 drops on the shoot tip growth point of the diploid cabbage plant (2n=2x=18) Colchicine solution, twice a day, once at 8:00 am to 9:00 am and once at 2:00 pm to 3:00 pm, for 5 consecutive days. The mutant strain of the first-generation tetraploid head cabbage variety Jixin was obtained, and its somatic chromosome number was 36. The yield of tetraploid was 10.2%. Compared with diploids, there are variations in various stages of plant growth. The vegetative organs such as roots, stems, leaves, and reproductive organs such as flowers and fru...

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Abstract

The invention relates to a method for culturing a tetraploid common head cabbage, which belongs to the technical field of plant breeding. A conventional diploid common head cabbage is adopted and is respectively processed once at 8-9 a.m. and 2-3 p.m. every day at the cotyledon period by a colchicine solution with the concentration being 0.15% of the mass-volume ratio. The processing method comprises the following steps: dripping 2 drops of colchicine solution on the growing point of the stem apex of the diploid common head cabbage by a micrometre syringe; processing for 5 days continuously, and taking the ambient air temperature from 20 to 25 DEG C in daytime / from 16 to 18 DEG C at night in the processing stage; and obtaining a first-generation tetraploid common head cabbage mutant withthe chromosome number of 36 by cytological chromosome number inspection. Compared with a diploid, nutritive organs such as roots, stems, leaves and the like, floral organs and fruit reproductive organs all show polyploid greatness, the root system and the sedge diameter are thickened, the weight of a single plant is increased, the leaves are thickened and embrittled and the color of the leaves isdarkened. Flower buds and petals are enlarged. Some breeding characteristics are changed, the bolting period is delayed and the podding rate and the seed setting number of procreant plants are reduced.

Description

1. Technical field [0001] The invention relates to a method for cultivating tetraploid head cabbage, which belongs to the technical field of new plant variety cultivation methods and is specially used for tetraploid head cabbage varieties. 2. Background technology [0002] Traditionally, no matter the hybrids of head cabbage are selected by self-incompatibility or male sterility, they are all diploid types, and they are mainly used for frying and eating. Domestic varieties and Asian varieties are of better quality, but the bulb leaves Thin and poor disease resistance; European varieties have thick bulb leaves and strong stress resistance, but poor quality. It is difficult to find varieties with thick bulbous leaves, crisp and tender, strong stress resistance, good quality and suitable for raw food. The tetraploid non-heading pakchoi variety proved to have obvious advantages over the diploid variety in terms of yield, leaf thickness and quality. The cytoplasmic male sterile...

Claims

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Application Information

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IPC IPC(8): A01H1/08
Inventor 严继勇高兵宋立晓曾爱松
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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