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Microbial inoculum and method for preparing biological organic fertilizer by using same

A microbial bacterial liquid and bacterial liquid technology, applied in the biological field, can solve the problems of excessive organic pollutants and additives, peculiar smell of bio-organic fertilizers, inconvenient storage and transportation, etc., and achieve low prices, increase organic matter content, and promote growth. Effect

Inactive Publication Date: 2012-07-25
迟仁立 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing bio-organic fertilizers are not comprehensive enough in nutrients, organic pollutants and additives exceed the standard, and cannot meet people's needs for pollution-free food and green organic food, and the obtained bio-organic fertilizers often have peculiar smell and are not convenient for storage and transportation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation of embodiment 1 biological organic fertilizer

[0023] (1) Preparation of culture medium: 10 parts of peptone, 3 parts of beef extract, 5 parts of NaCl, and 982 parts of water were uniformly mixed to make a culture medium.

[0024] (2) expanding Bacillus subtilis, Bacillus and Pichia pastoris respectively in culture medium;

[0025] The bacterial liquid obtained is mixed uniformly according to the quantity ratio of each bacterium: Bacillus subtilis: Bacillus: Pichia pastoris=12:48:24;

[0026] (3) Furfural slag, starchy amino acid residue, distiller's grains, and vinegar grains are crushed at a carbon-to-nitrogen ratio of 25:1 to make the main ingredient; grass carbon rich in organic matter and finely ground steel slag powder are mixed evenly to make auxiliary materials;

[0027] (4) The main material and the auxiliary material are mixed evenly in a ratio of 3:5.2 by weight, and the organic matter content, pH, nitrogen element, P 2 o 5 and K 2 O cont...

Embodiment 2

[0031] The preparation of embodiment 2 biological organic fertilizer

[0032] (1) Preparation of culture medium: 10 parts of peptone, 3 parts of beef extract, 5 parts of NaCl, and 982 parts of water were uniformly mixed to make a culture medium.

[0033] (2) expanding Bacillus subtilis, Bacillus and Pichia pastoris respectively in culture medium;

[0034] The obtained bacterial solution is uniformly mixed according to the quantitative ratio of each bacterium: Bacillus subtilis: Bacillus: Pichia pastoris = 8: 32: 16.

[0035] (3) Use soybean meal, rapeseed cake, manure, livestock and poultry manure to make the main material by crushing according to the carbon-to-nitrogen ratio of 25:1; use ground steel slag powder, electric heating plant fly ash, and weathered coal to mix evenly to make auxiliary materials;

[0036] (4) The main material and the auxiliary material are mixed evenly in the ratio of 4.8:7 by weight, and the organic matter content, pH, nitrogen element, P 2 o 5 ...

Embodiment 3

[0040] The preparation of embodiment 3 biological organic fertilizer

[0041] (1) Preparation of culture medium: 10 parts of peptone, 3 parts of beef extract, 5 parts of NaCl, and 982 parts of water were uniformly mixed to make a culture medium.

[0042] (2) expanding Bacillus subtilis, Bacillus and Pichia pastoris respectively in culture medium;

[0043] The bacterial liquid obtained is mixed uniformly according to the quantitative ratio of each bacterium: Bacillus subtilis: Bacillus: Pichia pastoris=10:40:20;

[0044] (3) Slaughter leftovers, Chinese herbal medicine slag, and waste fungus culture medium are crushed at a carbon-to-nitrogen ratio of 25:1 to make main ingredients; peat rich in organic matter and rare earth ore powder are evenly mixed to make auxiliary materials;

[0045] (4) The main material and the auxiliary material are mixed evenly in the ratio of 3.9:6.1 by weight, and the organic matter content, pH, nitrogen element, P 2 o 5 and K 2 O content, the org...

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Abstract

The invention relates to microbial inoculum which contains bacillus subtilis CIP3 CGMCC NO.3171, bacillus sp. CIP1 CGMCC NO.3172 and Pichia sp. CIP2 CGMCC NO. 3170. The invention also relates to a method for preparing biological organic fertilizer by using the microbial inoculum. Three bacteria which are all beneficial bacteria are mutually matched and have extremely strong decomposition capacityto organic matters, and the obtained decomposed products have no extraneous odors; and the three bacteria can improve the soil microenvironment, accelerate the growth of crops and reduce plant diseases and insect pests while being used in soil. The method for preparing the biological organic fertilizer has the advantages that the adopted raw materials are all agricultural and industrial leftovers, and the cost is low; the roles of three microbes can be fully exerted, various organic matters can be ensured to be decomposed, and the organic matter contents of soil are increased.

Description

technical field [0001] The invention relates to the field of biology, in particular to a microbial bacterial liquid and a method for preparing bio-organic fertilizer by using it. Background technique [0002] With the development of the economy and the improvement of living standards, people have higher and higher requirements for the quality of agricultural products. In order to reduce pollution, increase crop yields, and fertilize the soil at the same time, to achieve sustainable development. And produce real green organic food, which requires a lot of bio-organic fertilizers. Existing bio-organic fertilizers are not comprehensive enough in nutrients, organic pollutants and additives exceed the standard, and cannot meet people's needs for pollution-free food and green organic food, and the obtained bio-organic fertilizers often have peculiar smell and are not convenient for storage and transportation. Contents of the invention [0003] The technical problem to be solved...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C05F17/00C12R1/84C12R1/07C12R1/125
CPCY02W30/40
Inventor 迟仁立左淑珍迟彭
Owner 迟仁立