Microbial bacterial agent, preparation method thereof and method for treating animal excrements

A microbial agent and treatment method technology, applied in the field of microbial agent and its preparation, can solve the problems of long fermentation time, affecting the application of livestock and poultry manure, and the inability to effectively control and treat the odor of manure, and achieve the goal of solving pollution Effect

Active Publication Date: 2010-03-10
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the methods for treating livestock and poultry manure are composting, that is, under aerobic conditions, the microbial reproduction of the manure itself is used to ferment the manure. The disadvantage of this method is that the fermentation time is long, and the fermentation process has an impact on the environment.
[0004] In addition, people also add microbial agents to livestock a

Method used

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Examples

Experimental program
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preparation example Construction

[0020] The present invention also provides a preparation method of the microbial agent, wherein the method comprises inoculating Bacillus licheniformis, Bacillus subtilis, Bacillus pumilus, Bacillus coagulans, Saccharomyces cerevisiae and Streptomyces jingyang in the culture medium, The total viable count of the obtained microbial agent is 0.5-1×10 11 microbial bacterial agent per gram, wherein the number of viable bacteria of the Bacillus licheniformis is 10-20% of the total number of viable bacteria, the number of viable bacteria of the Bacillus subtilis is 10-20% of the total number of viable bacteria, The viable count of the Bacillus pumilus is 10-20% of the total viable count, the viable count of the Bacillus coagulans is 10-20% of the total viable count, and the viable count of the Saccharomyces cerevisiae is the total 5-15% of the number of live bacteria, and the number of live bacteria of Streptomyces jingyang is 5-15% of the total number of live bacteria.

[0021] In...

Embodiment 1

[0039] This example is used to illustrate the microbial inoculum provided by the present invention and its preparation method.

[0040] (1) Inoculate 3 parts by weight of the bacterial strain of Bacillus licheniformis (ACCC 10146, purchased from China Agricultural Microorganism Culture Collection Center) in 100 parts by weight of nutrient gravy medium, ferment and cultivate at 37 ° C, sample during fermentation and Observe by microscope direct counting method until the number of live bacteria of Bacillus licheniformis is 0.5×10 10 culture medium per gram, wherein, in the gravy medium, the content of peptone is 0.5% by weight, the content of NaCl can be 0.5% by weight, the content of beef extract can be 0.3% by weight, and the balance is sterile water, The pH is 7.0.

[0041] (2) Inoculate 4 parts by weight of the strain of Bacillus subtilis (ACCC 10629, purchased from the China Agricultural Microorganism Culture Collection Center) in 100 parts by weight of the nutrient gravy ...

Embodiment 2

[0048] This example is used to illustrate the microbial inoculum provided by the present invention and its preparation method.

[0049] (1) Inoculate 100 parts by weight of the nutrient gravy medium with 5 parts by weight of the bacterial strain of Bacillus licheniformis (ACCC 10146, purchased from the China Agricultural Microorganism Culture Collection Center), ferment and cultivate at 37 ° C, take samples during the fermentation and Observe by microscope direct counting method until the number of live bacteria of Bacillus licheniformis is 1×10 10 culture medium per gram, wherein, in the gravy medium, the content of peptone is 0.8% by weight, the content of NaCl can be 0.4% by weight, the content of beef extract can be 0.45% by weight, and the balance is sterile water, The pH is 7.0.

[0050] (2) Inoculate 100 parts by weight of the nutrient gravy medium with 3 parts by weight of the strain of Bacillus subtilis (ACCC 10629, purchased from the China Agricultural Microorganism...

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Abstract

The invention provides a microbial bacterial agent, a preparation method thereof and a method for treating animal excrements by using the microbial bacterial agent. The microbial bacterial agent can quickly decompose organic matters in the animal excrements, effectively remove odors of the animal excrements and reduce the content of harmful substances in the excrements. In addition, in the treatment method of the animal excrements, because the cultivation of animals is carried out on a base stock inoculated with the microbial bacterial agent, after the animal excrements are discharged, the harmful substances in the excrements can be directly discomposed by the fermentation of microorganisms in the base stock; thus, the animal excrements and the used base stock can be used as raw materials for producing organic fertilizers, the transformation from waste into the organic fertilizers, and the like of the animal excrements is finished, and the problem of environmental pollution caused byexcrements generated by scale animal cultivation is thoroughly solved.

Description

technical field [0001] The invention relates to a microbial agent, a preparation method thereof and a treatment method for livestock and poultry manure using the microbial agent. Background technique [0002] With the needs of the society, large-scale farming has become one of the new industries. But a very important problem in large-scale farming is the harmless treatment of livestock and poultry manure. Livestock and poultry manure is the main cause of environmental pollution by the breeding industry. Therefore, with the development of pollution-free, green and organic agriculture, the treatment of livestock and poultry manure has become an urgent problem to be solved. [0003] At present, most of the methods for treating livestock and poultry manure are composting, that is, under aerobic conditions, the microbial reproduction of the manure itself is used to ferment the manure. The disadvantage of this method is that the fermentation time is long, and the fermentation pro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C02F11/02C05F3/00C12R1/01C12R1/125C12R1/08C12R1/07C12R1/865C12R1/465
CPCY02A40/20
Inventor 朱昌雄刘波蓝江林李瑞波刘丽辉郭萍苏明星俞晓芸田云龙李锋叶婧刘雪
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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