Microbial bacterial agent, preparation method thereof and method for treating animal excrements
A microbial agent and treatment method technology, applied in the field of microbial agent and its preparation, can solve the problems of long fermentation time, affecting the application of livestock and poultry manure, and the inability to effectively control and treat the odor of manure, and achieve the goal of solving pollution Effect
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[0020] The present invention also provides a preparation method of the microbial agent, wherein the method comprises inoculating Bacillus licheniformis, Bacillus subtilis, Bacillus pumilus, Bacillus coagulans, Saccharomyces cerevisiae and Streptomyces jingyang in the culture medium, The total viable count of the obtained microbial agent is 0.5-1×10 11 microbial bacterial agent per gram, wherein the number of viable bacteria of the Bacillus licheniformis is 10-20% of the total number of viable bacteria, the number of viable bacteria of the Bacillus subtilis is 10-20% of the total number of viable bacteria, The viable count of the Bacillus pumilus is 10-20% of the total viable count, the viable count of the Bacillus coagulans is 10-20% of the total viable count, and the viable count of the Saccharomyces cerevisiae is the total 5-15% of the number of live bacteria, and the number of live bacteria of Streptomyces jingyang is 5-15% of the total number of live bacteria.
[0021] In...
Embodiment 1
[0039] This example is used to illustrate the microbial inoculum provided by the present invention and its preparation method.
[0040] (1) Inoculate 3 parts by weight of the bacterial strain of Bacillus licheniformis (ACCC 10146, purchased from China Agricultural Microorganism Culture Collection Center) in 100 parts by weight of nutrient gravy medium, ferment and cultivate at 37 ° C, sample during fermentation and Observe by microscope direct counting method until the number of live bacteria of Bacillus licheniformis is 0.5×10 10 culture medium per gram, wherein, in the gravy medium, the content of peptone is 0.5% by weight, the content of NaCl can be 0.5% by weight, the content of beef extract can be 0.3% by weight, and the balance is sterile water, The pH is 7.0.
[0041] (2) Inoculate 4 parts by weight of the strain of Bacillus subtilis (ACCC 10629, purchased from the China Agricultural Microorganism Culture Collection Center) in 100 parts by weight of the nutrient gravy ...
Embodiment 2
[0048] This example is used to illustrate the microbial inoculum provided by the present invention and its preparation method.
[0049] (1) Inoculate 100 parts by weight of the nutrient gravy medium with 5 parts by weight of the bacterial strain of Bacillus licheniformis (ACCC 10146, purchased from the China Agricultural Microorganism Culture Collection Center), ferment and cultivate at 37 ° C, take samples during the fermentation and Observe by microscope direct counting method until the number of live bacteria of Bacillus licheniformis is 1×10 10 culture medium per gram, wherein, in the gravy medium, the content of peptone is 0.8% by weight, the content of NaCl can be 0.4% by weight, the content of beef extract can be 0.45% by weight, and the balance is sterile water, The pH is 7.0.
[0050] (2) Inoculate 100 parts by weight of the nutrient gravy medium with 3 parts by weight of the strain of Bacillus subtilis (ACCC 10629, purchased from the China Agricultural Microorganism...
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