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Bacillus subtilis and propiconazole compound wettable sterilizing powder and use thereof

A technology of Bacillus subtilis and propiconazole, which is applied in the directions of fungicides, applications, biocides, etc., can solve the problems of excessive pesticide residues, pollute the ecological environment of orchards, and affect the quality of fruit products, achieve fast bacteriostatic speed, and improve control effects. , the control effect is stable

Inactive Publication Date: 2012-04-25
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to propose a method that has a good control effect on various fungal diseases of fruit trees in view of the problems that chemical pesticides are used in large quantities to prevent and control fruit tree diseases, seriously pollute the ecological environment of orchards, cause pesticide residues to exceed the standard, and affect fruit quality. Wettable fungicide powder compounded by Bacillus subtilis propiconazole microorganism and its application in the control of fruit tree fungal diseases

Method used

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  • Bacillus subtilis and propiconazole compound wettable sterilizing powder and use thereof
  • Bacillus subtilis and propiconazole compound wettable sterilizing powder and use thereof
  • Bacillus subtilis and propiconazole compound wettable sterilizing powder and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Inhibition of the growth of Bacillus subtilis sf-628 on the growth of Pythias spp.

[0021] The tested fungal pathogen strains: Pear ringworm, black spot fungus, and anthracnose bacteria were provided by the Plant Protection Institute of Jiangsu Academy of Agricultural Sciences. The pathogenic bacteria to be tested were transplanted on PSA medium (200g potato, 20g sucrose, 20g agar and 1000ml distilled water, pH 5.6-6.6), placed in an incubator at 28°C for 5 days, and set aside.

[0022] The biocontrol bacteria Bacillus subtilis sf-628 screened by Jiangsu Academy of Agricultural Sciences was placed on NA slope (3g beef extract, 1g yeast extract, 5g peptone, 10g sucrose, 20g agar and 1000ml distilled water, pH 6.8~7.0) After activation, transfer to 50ml NA culture medium, shake (150r / m) at 28°C for 48h, and set aside. Place the mycelium block of pathogenic bacteria in the center of the PSA plate, with a diameter of 7mm; place 4 pieces of filter paper diagonally around t...

Embodiment 2

[0026] Determination of Toxicity of Different Proportions of Active Pharmaceutical Ingredients against Pyrethia spp.

[0027] (1) For the test bacteria:

[0028] Pear ringworm was used as the test bacteria (provided by the Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences).

[0029] (2) Test drug:

[0030] A. 25% propiconazole EC was produced and provided by Jiangsu Fengdeng Pesticide Co., Ltd.

[0031] B. 60 billion Bacillus subtilis wettable powder is produced and provided by Jiangsu Suke Agrochemical Co., Ltd.

[0032] Indoor synergistic bioassay:

[0033] Make 25% propiconazole EC into 0.01μg / ml, 0.1μg / ml, 1μg / ml, 10μg / ml PSA medium plate with medicine; make 60 billion Bacillus subtilis wettable powder into 6×106 cfu Bacillus subtilis / ml, 6×10 5 cfu Bacillus subtilis / ml, 6×10 4 cfu Bacillus subtilis / ml, 6×10 3 cfu Bacillus subtilis / ml PSA medium plate with bacteria; A and B are compared according to the weight ratio of active drug ingredients 1:...

Embodiment 3

[0040] Preparation of 8% propiconazole · 10 billion Bacillus subtilis wettable powder

[0041] Effective drug: the sources of 25% propiconazole EC and 60 billion bacillus subtilis wettable powder are the same as in Example 2.

[0042] Auxiliary selection: NNO (sodium methylene dinaphthalene sulfonate).

[0043] Filling accessories: light calcium carbonate

[0044] Each component is prepared according to weight percentage:

[0045] 25% propiconazole: 32%; 60 billion Bacillus subtilis wettable powder: 16.7%; NNO (sodium methylene dinaphthalene sulfonate): 8%; light calcium carbonate: 43.3%.

[0046] Preparation process: put propiconazole and Bacillus subtilis in the mixer according to the above ratio, add NNO (sodium methylene dinaphthalene sulfonate), fill it with light calcium carbonate to 100%, and mix all materials in the mixer After uniformity, it is pulverized by a jet mill to obtain 8% propiconazole·10 billion Bacillus subtilis wettable powder.

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Abstract

The invention discloses bacillus subtilis and propiconazole compound wettable sterilizing powder. The finished medicament comprises active ingredients, assistants and a filler assistant, and is characterized in that: the active ingredients account for 10 to 85 percent of the total weight of the finished medicament and consist of the bacillus subtilis and the propiconazole; and the bacillus subtilis accounts for 10 to 90 percent of the weight of the active ingredients. To be used, the finished medicament is diluted and sprayed onto fruit trees to prevent and control fungal diseases of the fruit trees. The finished medicament has the advantages that: due to the use of the bacillus subtilis and the propiconazole which are mixed in a certain ratio, the synergy of microorganisms and chemical agents can be fully played, so as to complement mutual advantages, enlarge prevention and control range, reduce dosage of the chemical agents, reduce medicinal administration cost, improve prevention and control effect, improve the ecological environment in fruit gardens and reduce the residues of chemical agents in fruits.

Description

technical field [0001] The invention relates to a compound wettable bactericidal powder and its application, in particular to a wettable bactericidal powder composed of bacillus subtilis and propiconazole and its application in preventing and treating fungal diseases of fruit trees. Background technique [0002] With the rapid development of the national economy and the continuous improvement of people's living standards, higher and higher requirements are put forward for the quantity, quality and safety of fruits. In the past 20 years, my country's fruit tree cultivation has made great progress, the cultivated area has increased rapidly, the fruit output has increased rapidly, and the economic and social benefits are more obvious. Especially in the past 10 years, the adjustment of the rural industrial structure has brought about the gratifying situation of "thousands of households planting fruit trees". However, the current situation of fruit production is not optimistic. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01N43/653A01N25/14A01P3/00
Inventor 陈志谊刘邮洲刘永锋
Owner JIANGSU ACAD OF AGRI SCI
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