Plant extract formula for inhibiting aeromonas hydrophila

A technology of Aeromonas hydrophila and plant extracts, which is applied in the fields of plant raw materials, plant/algae/fungus/moss components, antibacterial drugs, etc. Achieve significant bacteriostatic effect and do not pollute the water environment

Active Publication Date: 2010-06-09
上海国矗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the limited effect of single medicine, it affects the therapeutic effect

Method used

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  • Plant extract formula for inhibiting aeromonas hydrophila
  • Plant extract formula for inhibiting aeromonas hydrophila
  • Plant extract formula for inhibiting aeromonas hydrophila

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The mensuration of embodiment 1 antibacterial activity

[0060] (1) Mix gallnut extract (50%-80%), tea polyphenols (10%-30%), and rhubarb extract (5%-15%), and use Aeromonas hydrophila as the test strain , the antibacterial effect of the prescription was determined by the filter paper method and the double dilution method, and each experiment was repeated three times.

[0061] Antibacterial effect: the diameter of the antibacterial zone is 26.42mm, the minimum inhibitory concentration is 78ug, and the minimum bactericidal concentration is 156ug.

Embodiment 2

[0062] Embodiment 2 preventive administration

[0063] The test was carried out in an aquarium of 80cm×60cm×50cm, with 1 / 3 of the water changed every day, and the water temperature was about 25°C. The test fish is crucian carp, with a body weight of 50 g, and it is temporarily kept in an aquarium for 7 days. After being identified as a healthy fish, the test begins. The bait was fed with 0.5%-1% compound extract, and the challenge test was started after 20 days of feeding. In the experiment, the positive control group with injection of Aeromonas hydrophila, the positive control group without injection of Aeromonas hydrophila and the negative control group without injection of Aeromonas hydrophila were respectively set up, and 20 carp were stocked in each test group. strip. Observed for 1 week, and counted the mortality rate of each group. Three repetitions per set.

[0064] A few hours after the injection of Aeromonas hydrophila, the body color of some test fish began to t...

Embodiment 3

[0066] Embodiment 3 therapeutic administration

[0067] The test was carried out in an aquarium of 80cm×60cm×50cm, with 1 / 3 of the water changed every day, and the water temperature was about 25°C. The test fish is crucian carp, with a body weight of 50 g, and it is temporarily kept in an aquarium for 7 days. After being identified as a healthy fish, the test begins. In the challenge test, the positive control group with injection of Aeromonas hydrophila, the positive control group with no injection of Aeromonas hydrophila and the negative control group with no administration and no injection of Aeromonas hydrophila were set up, and each test group was stocked with 20 carp. After challenge, feed with 0.5%-1% compound extract in the bait, observe for 1 week, and count the mortality of each group. Three repetitions per set.

[0068] A few hours after the injection of Aeromonas hydrophila, the body color of some test fish began to turn black, and then some fish died one after ...

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Abstract

The invention provides a plant extract formula for inhibiting aeromonas hydrophila, which belongs to the technical field of the development and the utilization of natural products. The formula comprises the following components in percentage by weight: 50 to 80 percent of Chinese gall, 10 to 30 percent of tea polyphenol and 5 to 15 percent of rhubarb extract. The plant extract compound formula prepared by the invention is formed by combining pure natural plant extracts, has the characteristics of significant antibacterial effect, no drug residues, no pollution to water body environments and the like, provides a new method for preventing and treating aquatic animal diseases caused by the aeromonas hydrophila, and can be used as Chinese medicament preparation and a feed additive.

Description

technical field [0001] A plant extract composition for inhibiting Aeromonas hydrophila belongs to the technical field of natural product development and utilization. Background technique [0002] Aeromonas hydrophila (Aeromonas hydrophila) is a pathogenic bacterium that causes aquatic animal diseases. It has a wide range of pathogenicity and is the most common pathogenic bacterium in fish. The morbidity rate reaches more than 90%, and the mortality rate is as high as more than 65%, which often causes heavy economic losses to the freshwater aquaculture industry. Species include heterogeneous gibel crucian carp, white crucian carp, crucian carp, bream, silver carp, bighead carp, dace, carp, mandarin mandarin fish, rice field eel, grass carp, white pomfret, California perch, silver pomfret, bearded catfish and other freshwater aquaculture fish. The disease is considered to be an infectious disease with the widest endemic area, the longest endemic season, the most types of fres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/708A61K9/16A61K9/14A61K9/12A61K9/08A61P31/04A61K35/64A61K31/353
Inventor 王洪新卢春霞吕文平
Owner 上海国矗生物科技有限公司
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