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Jerusalem artichoke pretreatment method for raw material for microbial fermentation culture

A technology for microbial fermentation and microbial culture, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., and can solve problems such as being unsuitable for use in the fermentation industry, low inulinase activity, and low reducing sugar yield. , to achieve the effect of improving the yield of the fermentation target product, improving the utilization rate of raw materials, and shortening the processing time.

Inactive Publication Date: 2010-06-09
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzymatic hydrolysis reaction conditions are mild but the reaction time is long (Li Jungang et al. Food Science. 1999, (1): 34-36), and the inulinase activity reported so far is not high (Singh R S et al. Bioresource Technology. 2007, 98 : 2518~2525)
Moreover, the cost of processing Jerusalem artichoke by enzymatic hydrolysis is relatively high, and it has no inactivation effect on other substances that inhibit the growth of microorganisms in the raw material
It is reported in the literature that the purpose of processing Jerusalem artichoke under acidic conditions is to obtain fructose or high fructose syrup. The operating temperature used is lower than 100 degrees Celsius, the acid hydrolysis time is long, and the yield of reducing sugar is low. Applied to fermentation industry (Hu Jiaqi et al. Guangzhou Chemical. 2007, 32(3): 46~50)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 100 grams of fresh Jerusalem artichoke tubers (total sugar mass content 17%) and 100 milliliters of water are mixed, pulverized and homogenized. Adjust the pH to 1.0 with the above-mentioned obtained Jerusalem artichoke pulp with 6mol / L sulfuric acid, and treat it at 105°C for 15 minutes. After cooling, take a sample of the hydrolyzate, and the analysis shows that the total sugar concentration is 93 g / L, and the monosaccharide concentration is 91 g / L. The hydrolysis rate is 97%.

Embodiment 2

[0027] 100 grams of fresh Jerusalem artichoke tubers (total sugar mass content 17%) and 100 milliliters of water are mixed, pulverized and homogenized. Adjust the pH of the aforementioned Jerusalem artichoke pulp to 1.0 with 6 mol / L hydrochloric acid, and treat it at 135°C for 2 minutes. After cooling, take a sample of the hydrolyzed solution. Analysis shows that the total sugar concentration is 93 g / L, and the monosaccharide concentration is 86 g / L. The hydrolysis rate is 92%.

Embodiment 3

[0029] Jerusalem artichoke powder (68% of total sugar mass content) 13 grams, 100 milliliters of water, adjust pH to 3.0 with 10 mol / liter sulfuric acid, handle 20 minutes at 140 ℃, after cooling, get hydrolyzate sample, analysis shows that total sugar concentration is 85 g / L, the monosaccharide concentration is 76 g / L, and the hydrolysis rate is 89%.

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Abstract

The invention relates to a pretreatment method of a jerusalem artichoke raw material, in particular to a method for processing jerusalem artichoke tubers and obtaining the raw material for microbial fermentation culture. The method comprises the steps of mixing jerusalem artichoke powder or fresh jerusalem artichoke tubers with water according to a certain weight ratio, homogenizing, adding acid for regulating pH to 1-4, processing at the temperature of 101 DEG C-140 DEG C for 2-20min, and obtaining hydrolysate; and using alkali for neutralizing the hydrolysate, regulating the pH to 5-8 and obtaining the raw material for microbial fermentation culture. The method has the advantages of short raw material treatment time, simple and easy operation, small energy consumption, low cost, high monosaccharide concentration in the hydrolysate, fast mycelial growth for microbial fermentation, high yield of a target product and low content of organic mattes in fermentation waste water, and can simultaneously carry out raw material treatment and culture medium sterilization, thereby providing an economic and feasible new technology for high-efficient utilizing the jerusalem artichoke raw material for carrying out microbial culture and bio-refining.

Description

technical field [0001] The invention relates to a pretreatment method for raw materials of Jerusalem artichoke, in particular to a method for processing the raw materials of Jerusalem artichoke to obtain raw materials for cultivating microorganisms, and belongs to the field of biotechnology. Background technique [0002] Jerusalem artichoke, also known as Jerusalem artichoke and ghost ginger, is a perennial herb of Compositae, which is suitable for growing in tidal flats and saline-alkali lands. The dry matter yield of Jerusalem artichoke tubers can reach 1.2 tons per mu, and the tubers are rich in inulin, which accounts for more than 70% of its dry weight. Inulin is a kind of fructan formed by linking D-fructofuranose through β-2,1-glucosidic bonds. ~50 fructose residues. Compared with other plants with high inulin content such as dahlia and chicory, the degree of polymerization (DP value) of inulin in Jerusalem artichoke tubers is expressed in the range of 3 to 50 (De Ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04C12P1/02C12R1/865C12R1/67C12R1/645C12R1/19
Inventor 赵宗保赵金张素芳沈宏伟
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI