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Method for extracting collagen polypeptide and hydroxyapatite in fish scales by cooking hot extrusion

A technology of fish scale collagen and hydroxyapatite, applied in chemical instruments and methods, phosphorus compounds, inorganic chemistry, etc., can solve the problems of unsuitable control of calcination temperature and time, waste of collagen, etc., and shorten working time and production cycle Short, pure effect

Inactive Publication Date: 2010-06-09
武汉普赛特膜技术循环利用有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of extracting hydroxyapatite from fish scales has been studied, but generally it is directly obtained by calcining fish scales. Since fish scales contain organic matter such as protein, the calcining temperature and time should not be controlled, and the waste of collagen is caused.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Add 20kg pulverization from hopper, the fish scale that water content is 8% (percentage by weight) in twin-screw extruder, the screw speed of twin-screw cooking extruder is 50 rev / mins, steam is heated to 110 ℃ and carries out hot extrusion, Hot extrude for 0.6 hours, add 160kg of water to the extrudate to extract collagen, and then filter, add trypsin to hydrolyze the filtrate accounting for 1% of fish scale weight, pH is 7.5, water bath heating temperature is 52°C, hydrolyze for 2.5h, enzymolyze After completion, heat to 90°C and keep for 10 minutes to inactivate the enzyme; decolorize the enzymolysis solution with activated carbon, remove impurities with an ultrafiltration membrane with a molecular weight cut-off of 3000 Dal, concentrate the permeate through a nanofiltration membrane with a molecular weight cut-off of 500 Dal, and freeze-dry to obtain fish scales Collagen polypeptide 6.44kg; the residue after collagen extraction was dried, pulverized, and then calcine...

Embodiment 2

[0025] Add 20kg pulverization from hopper, the fish scale that water content is 8% (percentage by weight) in twin-screw extruder, the screw speed of twin-screw cooking extruder is 80 rev / mins, steam is heated to 120 ℃ and carries out hot extrusion, Hot extrude for 0.5 hours, add 160kg of water to the extrudate to extract collagen, and then filter, add animal protease to hydrolyze the filtrate accounting for 1.5% of the weight of fish scales, pH is 7.5, and the heating temperature in the water bath is 52°C, hydrolyze for 2.5 hours, and the enzymolysis is completed After that, heat to 90°C and keep for 10 minutes to inactivate the enzyme; decolorize the enzymolysis solution with activated carbon, remove impurities with an ultrafiltration membrane with a molecular weight cut-off of 3000 Dal, concentrate the permeate through a nanofiltration membrane with a molecular weight cut-off of 500 Dal, and freeze-dry to obtain fish scale collagen Protein polypeptide 6.8kg; the residue after...

Embodiment 3

[0027] Add 20kg pulverization from hopper, the fish scale that water content is 8% (percentage by weight) in twin-screw extruder, the screw speed of twin-screw cooking extruder is 70 rev / mins, steam is heated to 120 ℃ and carries out hot extrusion, Hot extrude for 0.8 hours, add 200kg of water to the extrudate to extract collagen, and then filter, add alkaline protease which accounts for 3% of fish scale weight to hydrolyze the filtrate, the pH is 8, the heating temperature of the water bath is 52°C, hydrolyze for 2.5h, and enzymolyze After completion, heat to 90°C and keep for 10 minutes to inactivate the enzyme; decolorize the enzymolysis solution with activated carbon, remove impurities with an ultrafiltration membrane with a molecular weight cut-off of 3000 Dal, concentrate the permeate through a nanofiltration membrane with a molecular weight cut-off of 500 Dal, and freeze-dry to obtain fish scales Collagen polypeptide 6.58kg; the residue after collagen extraction was drie...

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Abstract

The invention relates to a method for extracting collagen polypeptide and hydroxyapatite in fish scales by cooking hot extrusion, which comprises the following steps: (a) pulverizing clean fish scales by a pulverizer; (b) putting the pulverized fish scales and water into a feed inlet of a double-screw cooking heat extruder for hot extrusion for 0.5-1h under the action of steam pressure to obtain thick slurry, wherein the content of the fish scales is 90%-95%; (c) after the treatment of the step b, adding water of which the weight is 8-10 times of the weight of the thick slurry, extracting collagen by water immersion, and filtering residues; (d) adding enzymes of which the weight is 1%-3% of the weight of the fish scales into the water solution of the collagen extracted in the step c for enzymolysis for 2-3h at 45-60 DEG C, heating to 90 DEG C after enzymolysis, keeping for 10min, and then inactivating the enzymes; (e) decoloring the enzymolysis solution obtained in the step d, removing impurities with an ultrafiltration membrane, concentrating the solution with a nanofiltration membrane, and freeze-drying the solution to obtain the collagen polypeptide of the fish scales; and (f) drying and pulverizing the residues obtained in the step c, and then, putting the residues in a muffle furnace for calcining for 1-2h at 700-1000 DEG C to obtain nano-hydroxyapatite. The invention does not need acid-base pretreatment, has good separation and purification effects and can not influence the activity of the collagen polypeptide and the hydroxyapatite in the fish scales.

Description

technical field [0001] The invention relates to a method for preparing collagen polypeptide and hydroxyapatite from fish scales, in particular to a method for conveniently separating and extracting collagen polypeptide and hydroxyapatite in fish scales through cooking and hot extrusion. Background technique [0002] Collagen peptides are hydrolyzed products of collagen, generally with a molecular weight below 3000Dal. Collagen peptides have many physiological functions. The digestion and absorption rate of collagen peptides is almost 100%. It can protect gastric mucosa and anti-ulcer, promote skin collagen metabolism, inhibit blood pressure rise, and promote Ca 2+ Absorb and reduce serum cholesterol content, anti-oxidation, anti-aging, etc. Hydroxyapatite is the main component of inorganic substances in human bone tissue, accounting for 90% by mass, and the other 10% is calcium carbonate and other inorganic salts. The molecular formula is Ca 10 (PO 4 ) 6 (OH) 2 , the mo...

Claims

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Application Information

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IPC IPC(8): C12P21/06C01B25/32
Inventor 万端极何昌义徐国念魏旋杨青龚琴李丹
Owner 武汉普赛特膜技术循环利用有限公司
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