Tambac inducer and preparation method thereof

A kind of agarwood inducer and technology of agarwood, applied in botany equipment and methods, biocide, animal repellent, etc., can solve the problems of high price and unguaranteed supply, achieve low cost, good market application prospect, shorten The effect of forming time

Active Publication Date: 2010-06-16
HAINAN XIANGSHU ALOES IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And so far, the development and utilization of agarwood all rely on the collection of wild...

Method used

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  • Tambac inducer and preparation method thereof
  • Tambac inducer and preparation method thereof
  • Tambac inducer and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0026] This embodiment provides an agarwood inducer, which includes the living stem of Passiflora japonica, PDA medium, potato sucrose medium and agaricus argentina base.

[0027] The white wood base is composed of the following percentage components: 73% white wood powder, 25% bran, 1% sucrose, 0.5% calcium carbonate, 0.2% (NH 4 ) 2 HPO 4 , 0.2%K 2 HPO 4 , 0.1% MgSO 4 ·7H 2 O.

Embodiment 2

[0029] The present embodiment provides a kind of preparation method of agarwood inducer, the method comprises the following steps:

[0030] Step 101 Sampling and isolation and cultivation of agarwood-induced fungi;

[0031] Cut off the part that is forming agarwood (see figure 2 ), cut into small pieces of 3×3mm. Sterilize with 75% ethanol for 2 min, 0.1% mercury liter for 1 min, wash 4 times in sterile water, blot dry with sterilized filter paper, place on a PDA medium plate for dark cultivation at 25°C, and after colonies grow, pick The mycelial tip method was used to purify isolated fungi. The purified fungi were stored in PDA inclined test tubes containing liquid paraffin.

[0032] Step 102 identification of agarwood-induced fungi;

[0033] Add 20ml of PDA to a petri dish with a diameter of 10cm to form a flat plate, inoculate a colony edge mycelium block with a diameter of 7mm, and observe the colony characteristics of Trichodispora cocoa for different days. The res...

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Abstract

The invention discloses a tambac inducer and a preparation method thereof. The tambac inducer comprises an aquilaria sinensis tree vivo stem, a PDA culture medium, a potato sucrose culture medium and an aquilaria sinensis matrix. The method comprises the following steps of cutting off a part of an aquilaria sinensis tree vivo stem, which is forming tambac, and sterilizing, culturing, separating and identifying to obtain a tambac inducible strain; activating the strain by the PDA culture medium and fermenting by the potato sucrose liquid culture medium, and then inoculating the strain to the aquilaria sinensis matrix, and culturing for 3-5 days under the conditions of constant temperature of 30 DEG C, darkness and ventilation, thus obtain the tambac inducer. The invention has simple method, can remarkably shorten the forming time of the tambac, enhance the cultivating benefit of aquilaria sinensis trees and has favorable market application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an agarwood inducer and a preparation method thereof. Background technique [0002] Aquilaria sinensis (Lour.) Gilg, also known as Aquilaria sinensis, belongs to the genus Aquilaria in the family Daphneaceae. It is a unique tropical and subtropical evergreen tree in my country. It is a national second-class endangered protected plant. Agarwood is a traditional precious medicinal material and a precious natural spice in my country, Japan, India and other Southeast Asian countries. It is pungent, bitter, and slightly warm in nature, and can promote qi and relieve pain, warm the middle and stop vomiting, and relieve asthma (Pharmaccopoeia Committee of the People's Republic of China. Zhonghua Pharmacopoeia of the People's Republic of China (Part One) [S]. Beijing: Chemical Industry Press, 2005, 128.). There are two main components in Agarwood, sesquiterpenoids and chromones (Yang Junshan...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P21/00
Inventor 魏建和张争杨云孟慧高志晖陈伟平冯锦东陈怀琼
Owner HAINAN XIANGSHU ALOES IND
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