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Method for producing high-purity glabridin

A glabridin, high-purity technology, applied in organic chemistry and other directions, can solve problems such as difficulty in achieving purity, and achieve the effects of improved yield, good solubility, and high product safety.

Active Publication Date: 2010-06-16
广州市康伦生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the products obtained by the current domestic production process are difficult to achieve this purity, and toxic organic solvents such as chloroform and methanol are often used in production

Method used

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  • Method for producing high-purity glabridin
  • Method for producing high-purity glabridin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Glycyrrhiza glabra (collected from Xinjiang) was dried and crushed, and 1kg of powder passed through a 20-mesh sieve was taken, placed in a 10L stainless steel pot, decocted and extracted with 4L ammonia water of pH 8, boiled and stirred for 1 hour, and extracted twice in total, Centrifuge to remove the residue; combine the extracts and concentrate to 1L to obtain the concentrated ammonia solution;

[0030] (2) Put the ammonia water concentrate obtained in step (1) on the macroporous adsorption resin (AB-8, produced by Nankai University Chemical Factory) chromatography column (Φ60×600mm), after elution with 1500ml water, continue to use 2000ml volume percentage concentration For 45% ethanol elution, collect eluent, concentrate to dryness, obtain purity and be 56.02% glycyrrhizic acid, deposit; Continue to use 2000ml volume percent concentration as 95% ethanol elution, collect eluent, concentrate to 250ml, obtain a concentrate;

[0031] (3) Go up polyamide (Zhejiang...

Embodiment 2

[0035] (1) Get 10kg of Glycyrrhiza glabra (collected from Xinjiang) powder with a 20-mesh sieve, put it in a 100L reaction kettle, boil and stir for 1 hour with 50L of pH8.5 sodium hydroxide alkaline aqueous solution, and extract 3 times in total. Centrifuge to remove the residue; combine the extracts and concentrate to 15L to obtain the sodium hydroxide concentrate;

[0036] (2) Put the sodium hydroxide concentrated solution on the macroporous adsorption resin (X-5, produced by Nankai University Chemical Factory) chromatography column (Φ100×1500mm), after elution with 12L water, continue to use 20L volume percentage concentration to be 55% ethanol elution, collect the eluent, concentrate to dryness, and obtain glycyrrhizic acid with a purity of 63.55%, store it; continue to elute with 20L volume percentage concentration of 85% ethanol, collect the eluate, concentrate to 3L, and obtain a concentrated solution ;

[0037] (3) Put the 3L concentrated solution obtained in step (2...

Embodiment 3

[0041] (1) Get 50kg of Glycyrrhizae glabra (gathered from Xinjiang) powder 20 mesh sieves, place in a 500L multifunctional extraction tank, boil and stir for 2 hours with 250L pH9 potassium hydroxide alkali aqueous solution, extract 3 times altogether, Centrifuge to remove the residue; combine the extracts and concentrate to 80L to obtain potassium hydroxide concentrate;

[0042] (2) Put the concentrated solution of potassium hydroxide on a macroporous adsorption resin (AB-8, produced by Nankai University Chemical Factory) chromatography column (Φ300×1800mm), after eluting with 100L water, continue to use 200L volume percentage concentration of 50 % ethanol for elution, collect the eluent, concentrate to dryness, and obtain glycyrrhizic acid with a purity of 61.43%, store it; continue to elute with 200L volume percentage concentration of 90% ethanol, collect the eluate, concentrate to 30L, and obtain concentrated liquid;

[0043] (3) Add polyamide (produced by Zhejiang Taizho...

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Abstract

The invention discloses a method for producing high-purity glabridin. The method comprises the following steps: drying and crushing glycyrrhiza glabra, decocting the glycyrrhiza glabra with alkaline water, and filtering and concentrating the decoction to obtain alkaline water decoction; loading the decoction onto a resin chromatographic column, and eluting the resin chromatographic column with water and ethanol in turn; collecting 45 to 55 percent ethanol eluent, and concentrating the eluent to obtain glycyrrhizic acid; collecting 85 to 95 percent ethanol eluent, concentrating the eluent, loading the concentrated solution onto a polyamide chromatographic column, and eluting the polyamide chromatographic column with the ethanol of different percentage concentrations by volume in turn; collecting 70 to 75 percent ethanol eluent, and concentrating the eluent to be dry so as to obtain the glabridin; and refluxing and dissolving the glabridin into acetone, and cooling, crystallizing, filtering and drying mixture to obtain a finished product. According to the method, the alkaline water is used to replace an organic solvent for extraction, a macroporous resin column is used to replace a silica chromatographic column for separation, and the ethanol solution is used to replace the mixed eluent such as chloroform and the like, so the cost is lower, the safety of the product is better, and the purity and the yield of the product are higher.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine and cosmetics, in particular to a new method for producing high-purity glabridin from licorice glabra. Background technique [0002] Glabridin is a natural active ingredient extracted from a specific species of licorice - Glycyrrhizae glabra, which has the functions of antibacterial, anti-inflammatory, anti-oxidation, anti-aging, anti-ultraviolet rays, whitening and brightening, and freckle removal. Glabridin can not only inhibit the activity of tyrosinase, but also inhibit the interconversion of dopachrome and the activity of DHICA oxidase. It has the ability to scavenge oxygen free radicals similar to SOD (superoxide dismutase) and has a Similar ability to resist oxygen free radicals. It can penetrate deep into the skin and maintain high activity, effectively inhibiting the activity of various enzymes in the process of melanin production. At the same time, it also has the effect of ...

Claims

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Application Information

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IPC IPC(8): C07D493/04
Inventor 胡长鹰
Owner 广州市康伦生物技术有限公司
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