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Lactobacillus leavening agent, preparation method thereof and special bacterial strain

A technology of lactobacillus and starter, which is applied in the field of lactobacillus starter and its preparation and special strains, can solve the problems of limited development and utilization of resources, and achieve low syneresis sensitivity, high water holding capacity and satisfactory appearance Effect

Inactive Publication Date: 2012-01-04
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] There are many kinds of natural fermented products in our country, and each region has its unique fermented food, but the development and utilization of these resources is relatively limited, so the lactic acid bacteria that produce extracellular polysaccharides are separated and screened from these resources to prepare starter for the production of fermented milk products It has practical significance and helps to solve practical problems encountered in the production of fermented milk

Method used

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  • Lactobacillus leavening agent, preparation method thereof and special bacterial strain
  • Lactobacillus leavening agent, preparation method thereof and special bacterial strain
  • Lactobacillus leavening agent, preparation method thereof and special bacterial strain

Examples

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Effect test

Embodiment 1

[0024] Example 1. Isolation of Lactobacillus plantarum SC79 CCTCC M 208151

[0025] Separation medium (MRS medium): peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, KH 2 PO 4 2g / L, anhydrous sodium acetate 5g / L, sodium citrate 5g / L, MgSO 4 ·7H 2 O 0.2g / L, MnSO 4 4H 2 O0.05g / L, Tween 801mL, agar 15g / L, glucose 20g / L, pH6.6, sterilized at 121°C for 15min

[0026] Take 1mL sauerkraut juice and put it into 11g / 100mL skim milk, incubate at 37°C, take samples after curdling and dilute step by step, choose 3 dilutions, take 0.1mL for each dilution and spread on MRS agar plate, and incubate at 37°C for 48h , select a suitable plate, pick the typical colonies on it for Gram staining and catalase test, Gram staining is positive, observed under the microscope as rod-shaped, catalase test-positive bacterial strains are initially identified as milk bacillus, continue to streak culture to isolate and purify until pure bacteria are obtained.

[0027] The exopolysaccharide produ...

Embodiment 2

[0039] Embodiment 2, lactobacillus starter

[0040] 1. Optimization of the production process of Lactobacillus starter

[0041] a. Optimizing culture medium

[0042] The present invention selects whey medium rich in nutrition and easy for cell separation as the basic medium. In the basic whey medium, the number of live bacteria of Lactobacillus plantarum SC79 CCTCC M 208151 after culturing at 37°C for 16 hours is 6.2×10 9 cfu / mL.

[0043] I Optimization of carbon source

[0044] Replace glucose with maltose, lactose, fructose, sucrose, mannitol, and sorbitol in the basal medium, and measure the OD of the fermentation broth after culturing for 16 hours 600 , The number of live bacteria.

[0045] The test results showed that the number of live bacteria of Lactobacillus plantarum SC79 CCTCCM 208151 was the highest when adding glucose (2g / 100mL) to the basal medium.

[0046] Based on the above experimental results, 2g / 100mL glucose was the best carbon source for the growth of...

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Abstract

The invention discloses a lactobacillus leavening agent, a preparation method thereof and a special bacterial strain. The preservation serial number of lactobacillus is CCTCC M208151. An active constituent of the lactobacillus leavening agent is the lactobacillus. The invention also discloses a method for preparing the lactobacillus leavening agent, which comprises the following steps: firstly, leavening and culturing lactobacillus plantarum SC79 CCTCC M208151 in SC79 optimal culture medium; and secondly, collecting thallus in the step 1 and then adding a protective agent to the thallus to obtain the leavening agent. The lactobacillus leavening agent has small dosage, quick acid production speed through mixing the lactobacillus leavening agent and a harvestless exopolysacchatide yoghourt bacterial strain to prepare yoghourt, better relative viscosity, elasticity, denseness and adhesiveness than that of the conventional yogurt and small syneresis sensibility of formed sticky yogurt colloids, larger water holding capacity than that the conventional yogurt and hard whey separation.

Description

technical field [0001] The invention relates to a lactobacillus starter, a preparation method thereof and a special bacterial strain. Background technique [0002] Lactic acid bacteria (LAB) are GRAS (Generally recognized as safe) microorganisms that are beneficial to human health. Some lactic acid bacteria can secrete exopolysaccharides (exopolysaccharides, EPS). These exopolysaccharides adhere to the surface of the bacteria as capsular polysaccharides (CPS) or are secreted into the extracellular environment in the form of ropypolysaccharides. The exopolysaccharides produced by different types of lactic acid bacteria are also different, with various structural changes and biological activities. Lactic acid bacteria exopolysaccharides can protect bacterial cells against desiccation, phage and antibiotic invasion, resist antibiotics or toxic substances, help bacteria adhere to solid surfaces and participate in cell-cell interactions, etc. [0003] Lactic acid bacteria produ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/04A23C9/127C12R1/25
Inventor 杨贞耐张雪赵玉娟李达牛春华
Owner JILIN ACAD OF AGRI SCI
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