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Polypeptide for detecting human cancer cells, and application thereof

A cancer cell and polypeptide coupling technology, which is applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, peptides, etc., can solve the problems of poor stability, weak penetration, cumbersome preparation, etc., and achieve the goal of combining High capacity, good specific effect

Active Publication Date: 2012-01-11
INST OF CHEM CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, although antibodies are widely used in clinical diagnosis due to their specific targeting, they have disadvantages such as cumbersome preparation, poor stability, high cost, and weak penetration.

Method used

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  • Polypeptide for detecting human cancer cells, and application thereof
  • Polypeptide for detecting human cancer cells, and application thereof
  • Polypeptide for detecting human cancer cells, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Polypeptides specifically binding to human cancer cells

[0042] 1) Construction of affinity screening system

[0043] Polyglycidyl methacrylate (PGMA) microspheres (10 microns) of non-porous, particle diameter monodisperse are prepared with glycidyl methacrylate and ethylene glycol diester methacrylate copolymerization, method is with reference to following document: R.Zhao, C.L.Fang, X.Yu, Y.Liu, J.Luo, D.H.Shangguan, S.X.Xiong, G.Q.Liu, Screening of inhibitors for influenza A virus using high performance affinity chromatography and combinatorial peptide libraries, J.Chromatogr.A, 2005 , 1064:59-66.

[0044] The high-efficiency affinity chromatography filler was prepared by using the above-mentioned PGMA microspheres as a solid phase matrix.

[0045] With 0.2mol / L H 2 SO 4 Treat the above-mentioned PGMA microspheres for 6 hours to open the epoxy group on the surface; then mix the ring-opened PGMA microspheres with 2.4mol / L NaOH aqueous solution, add epi...

Embodiment 2

[0060] Embodiment 2, human liver cancer rapid diagnostic reagent

[0061] 1) Antisense peptide AP2H-fluorescein isothiocyanate (FITC) conjugate

[0062] Carbonate buffer (0.09mol / L NaHCO 3 , 0.01mol / L Na 2 CO 3 , 0.126mol / L NaCl; pH 9.0) dissolve antisense peptide AP2H to obtain antisense peptide AP2H solution, its concentration is 2mg / mL; fluorescein isothiocyanate (FITC) is dissolved in dimethyl sulfoxide (DMSO), Its concentration is 12.5 mg / mL. The antisense peptide AP2H solution and FITC solution were mixed at a volume ratio of 2:1, and reacted at room temperature in the dark; after 2 hours, the reaction product was purified by high performance liquid chromatography.

[0063] Using C 8 A reverse-phase chromatographic column (250nm×10mm i.d.) was used to separate and purify the AP2H-FITC conjugate in the reaction product. Mobile phase is; A: 0.1% (volume percentage) trifluoroacetic acid aqueous solution, B: 0.1% (volume percentage) trifluoroacetic acid acetonitrile solu...

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Abstract

The invention discloses polypeptide for detecting human cancer cells, and application thereof. The polypeptide consists of 1), 2), 3) or 4) as follows: 1) at least five amino acid residues from an amino terminal of a sequence 1 in a sequence table; 2), at least five amino acid residues from an amino terminal of a sequence 2 in the sequence table; 3) at least five amino acid residues from an aminoterminal of a sequence 3 in the sequence table; and 4) at least five amino acid residues from an amino terminal of a sequence 4 in the sequence table, wherein the polypeptide also contains substituent groups which are akoxyl groups, alkane acyl groups or amide groups, and the amino acid residues forming the polypeptide are L-type and / or D-type. The polypeptide overcomes the disadvantages of troublesome preparation, poor stability, high expenses, weak penetrating power and the like of antibodies and other biological preparations, and can be used for preparing diagnostic reagents and therapeutic medicaments for liver caner and other malignant tumors.

Description

technical field [0001] The invention relates to a polypeptide for detecting human cancer cells and its application. Background technique [0002] Hepatocellular carcinoma (HCC) is a common malignant tumor worldwide. High morbidity, high mortality, high degree of malignancy, late diagnosis, poor prognosis, and high recurrence rate are the characteristics of liver cancer. Therefore, it is of great significance to establish an early screening method for liver cancer and to find new effective anticancer drugs. [0003] At present, the preferred marker for serological diagnosis of liver cancer is alpha-fetoprotein (AFP), and the use of AFP antibody to detect AFP in serum has been used for many years. However, according to authoritative statistics, AFP can also be detected in the serum of some patients with liver cirrhosis or chronic hepatitis; at the same time, about 1 / 3 of HCC patients, their serum AFP is negative (<20ng / mL). Therefore, there are still defects in the speci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K17/00G01N33/68A61K38/08A61K47/48A61P35/00A61K47/64
Inventor 赵睿黄嫣嫣周柔丽
Owner INST OF CHEM CHINESE ACAD OF SCI
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