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Haematococcus pluvialis cell growth promoting agent and use method thereof

A growth promoter, the technology of Haematococcus pluvialis, applied in the directions of plant growth regulators, plant growth regulators, biocides, etc., can solve problems such as the growth effect of Haematococcus pluvialis that has not yet been seen, and achieve the promotion of cell growth and astaxanthin. Accumulation of hormones, increasing cell growth rate, and obvious effect of promoting growth

Active Publication Date: 2010-08-18
云南爱尔发生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there has been no research on the growth-promoting plant hormones and their regulatory substances (such as auxin and cytokinin) on the growth of Haematococcus, and there is no reasonable combination of these two types of hormones to regulate the cell growth and astaxanthin of Haematococcus. Discussion on Physiological Processes such as Toxin Accumulation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Naphthaleneacetic acid (NAA) is fully dissolved through 95% (volume percent) alcohol, and 3-indolebutyric acid (3-IBA) is fully dissolved through concentration of hydrochloric acid of 1mol / L simultaneously, then with 1 liter of distilled water above-mentioned mixed The homogenized NAA and 3-IBA are fully mixed to obtain the accelerator.

[0018] Then according to the preparation of Haematococcus nutrient solution of the revised formula of the MCM such as Zhang Jingpu (1994). Four strains of Haematococcus pluvialis, all in the vigorous logarithmic growth phase, were inoculated respectively (the strains were from the Institute of Oceanology, Chinese Academy of Sciences) and numbered H 0 、H 2 、H 3 、H 4 (citing literature).

[0019] Sun YH, Liu JG et al., 2008, A strain H 2 -419-4 of Haematococcus pluvialis induced by Ethyl Methanesulphonate and Ultraviolet radiation. Chin J. Ocean Limn, 26(2): 152-156.

[0020] Li Yingyu, Liu Jianguo, 2006, The relationship between t...

Embodiment 2

[0027] In the summer of 2008, four algae strains selected in Example 1 were inoculated and expanded in the vigorous logarithmic growth phase in Chuxiong City, Yunnan Province. A test of the amplification argument for cumulative effects. Haematococcus cell numbers are H 0 、H 2 、H 3 and H 4 , the verification uses indoor diffuse light as the light source, the illumination time is 12±0.5h, and the maximum illumination intensity is 800μmol m ？2 the s ？1 , the cultivation temperature is 24±2°C, the cultivation reactor is a 5L Erlenmeyer flask, the cultivation mode of Haematococcus pluvialis is aeration cultivation, the gas fed is a mixed gas composed of 0.55% carbon dioxide and air, and the ventilation volume is 2.50L min -1 .

[0028] After comparative analysis, the cell growth promoter can not only improve the rate of cell auxin, but also increase the accumulation of astaxanthin. where H 0 、H 2 、H 3 and H 4 The cell growth increasing effects of the algae strains were ...

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Abstract

The invention belongs to the technical field of biology, in particular to a haematococcus pluvialis cell growth promoting agent and a use method thereof. Concretely, rhodofix dissolved by ethanol and 3-seradix dissolved by hydrochloric acid are diluted into distilled water to obtain the promoting agent, wherein each liter of the solution has the rhodofix content between 0.5 and 3.0 ppm, and the 3-seradix content between 1.0 and 5.0 ppm. The obtained promoting agent is added into a culture medium of the haematococcus pluvialis cells. The invention uses the characteristic that the plant growth regulating substances have the features of promoting the cell division, the cell propagation and the growth in a certain concentration range. Through the analysis on the effect of the cell growth speed of different haematococcus pluvialis strain cells under different plant growth regulating agents and concentration matching processing, the grow promoting agent with the improved broad-spectrum effect on the haematococcus pluvialis cell growth and the proper concentration of each ingredient can be obtained through integration and optimization. The haematococcus pluvialis cell growth promoting agent of the invention has simple use method, and has the obvious promoting effect on the haematococcus pluvialis cell growth speed and the astaxanthin accumulation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a growth promoter for Haematococcus pluvialis cells and a method for using the same. Background technique [0002] Astaxanthin is an oxidized carotenoid derivative with wide application value and development prospects. It has two major characteristics: one is bright red and has strong coloring ability. Second, it has strong biological activity and physiological function, and its reducing ability is 10 times that of β-carotene and 100-550 times that of vitamin E! Known as "Super Vitamin E". Studies have shown that astaxanthin has a strong ability to capture free radicals and anti-oxidation, and also has a strong ability to enhance the body's immunity, anti-aging function, prevent cancer, and has the function of absorbing ultraviolet rays and preventing photodamage. Therefore, astaxanthin has wide application prospects in the fields of cosmetics, health care products and p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/38A01N37/10A01P21/00C12N1/12C12N1/38
Inventor 刘建国韩春梅张勇
Owner 云南爱尔发生物技术股份有限公司
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