Biological detoxification method of cottonseed meal

A cotton meal and biological technology, applied in the field of biological detoxification of cotton meal, can solve the problems of complex process, poor microbial adaptability, unstable detoxification effect, etc., and achieve simple production process, no three wastes, and poor palatability Effect

Active Publication Date: 2010-08-25
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the biological detoxification method has achieved good results, there are still disadvantages such as unstable detoxification effect, complicated process and poor microbial adaptability.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The strains used in Example 1 are 1 strain of Bacillus subtilis, 1 strain of Bacillus lateralis, 1 strain of Bacillus lateralis, 1 strain of Bacillus natto, 1 strain of Bacillus cereus and 1 strain of Saccharomyces yeast. The steps are:

[0022] (1) Preparation of liquid strains: Take 10g glucose, 5g peptone, 3g beef extract, 3g sodium chloride and 1000mL tap water (pH7.2) and stir well to form Bacillus subtilis, Bacillus lateralis, Bacillus natto and wax The liquid expansion broth nutrient medium of Bacillus spp. was sterilized under the pressure of 0.1MPa for 25 minutes, cooled to 30°C and inserted into the cultured mature slant strains, and cultured on a shaking table at 37°C and 180r / min respectively. 24h; Take 20g of glucose, 1.5g of yeast extract, 1g of potassium chloride, 8g of sodium acetate and 1000mL of tap water (pH5.5) and mix well to form a yeast liquid medium, sterilize under 0.1MPa pressure for 25min, and cool to 30°C Then insert the cultured mature slan...

Embodiment 2

[0026] The bacterial species used in this example are 1 strain of Bacillus subtilis, 1 strain of Bacillus lateraporus, 1 strain of Bacillus subtilis natto, 1 strain of Bacillus cereus and yeast of the genus Saccharomyces 1 strain of Saccharomyces. The steps are:

[0027] (1) Preparation of liquid strains: Take 15g glucose, 5g peptone, 5g beef extract, 3g sodium chloride and 1000mL tap water (pH7.5) and stir well to form Bacillus subtilis, Bacillus lateralis, Bacillus natto and wax The liquid expansion broth nutrient medium of Bacillus spp. was sterilized under 0.1MPa pressure for 25 minutes, cooled to 30°C and inserted into the cultured mature slant strains, and cultured on a shaking table at 30°C and 220r / min respectively. 36h; Take 30g of glucose, 1.7g of yeast extract, 1g of potassium chloride, 8.5g of sodium acetate and 1000mL of tap water (pH5.0) and mix well to form a yeast liquid medium, sterilize under 0.1MPa pressure for 25min, and cool to 30 After inserting the cul...

Embodiment 3

[0031] The bacterial species used in this example are 1 strain of Bacillus subtilis, 1 strain of Bacillus lateraporus, 1 strain of Bacillus subtilis natto, 1 strain of Bacillus cereus and yeasts of the genus Saccharomyces (Saccharomyces) 1 strain. The steps are:

[0032] (1) Liquid strain preparation: Bacillus subtilis, Bacillus lateralis, Bacillus natto and Bacillus cereus were composed of 20g glucose, 10g peptone, 4g beef extract, 4g sodium chloride and 1000mL tap water (pH7.4) Liquid culture medium for bacteria: take 40g glucose, 2.0g yeast extract, 1.5g potassium chloride, 9g sodium acetate, and 1000mL tap water (pH4.5) to form a yeast liquid culture medium. Sterilize under the pressure of 0.1MPa for 25min, cool to 30°C and insert the cultured mature slant strains, respectively at 28°C (yeast) and 35°C (bacteria) and 200r / min, shaker culture for 28h, as Production strains of solid strains.

[0033] (2) Preparation of solid bacteria: take 55kg of wheat bran, 35kg of oil ...

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PUM

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Abstract

The invention discloses a biological detoxification method of cottonseed meal, comprising: adding ferrous sulfate heptahydrate, 40,000-60,000 U/g of neutral xylanase, microorganism strains and the like into smashed cottonseed meal; after being evenly stirred, jointly acting for 4-5 days by microorganism and neutral xylanase at the temperature of 45-55 DEG C; and drying and smashing to obtain the product. The free gossypol content of the obtained product is lowered than the safety index specified by feed, the nutritional value thereof is higher than that of unleavened cottonseed meal, product protein content reaches 45-50%, free amino acid content is above 15%, and the cottonseed meal can be directly used as protein feed. The invention better solves the problems of incomplete detoxification of cottonseed meal, protein denaturation and secondary feed pollution due to adopting the chemical detoxification method in the prior art, and provides a non-toxic cottonseed meal production method which has favorable detoxification effect and low technical energy consumption and can reach feed standard requirements.

Description

technical field [0001] The invention relates to a detoxification method for cotton meal, in particular to a biological detoxification method for cotton meal. Background technique [0002] my country is a large cotton-producing country, which can provide about 6 million tons of cottonseed each year, and the total output of cottonseed cake (cotton meal) is about 3 million tons, accounting for about 30% of the output of various plant cakes. Cottonseed meal is a by-product of extracting cottonseed oil, and it is used as feed, which has the characteristics of protein feed and energy feed. The crude protein content of cottonseed cake is relatively high, reaching more than 34%, and the crude protein content of cottonseed cake can reach 41%-60%. Judging from the amino acid content of cottonseed meal, it has 8 essential amino acids, lysine is as high as 6%, and the content of cystine and tryptophan is relatively rich; the content of arginine is much higher than that of soybean meal,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14
Inventor 肖调义兰时乐王红权金红春
Owner HUNAN AGRICULTURAL UNIV
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