Preparation method for high purity fish scale collagen

A technology of fish scale collagen and collagen, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve problems such as unstable product quality, low product purity, and large waste water discharge, and achieve acid saving Alkali soaking and cleaning steps, efficient separation effect, process controllable effect

Active Publication Date: 2010-08-25
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the traditional process only relies on acid-base soaking to remove impurities, the final product is not pure and the product quality is not stable
The traditional process needs to repeat the

Method used

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  • Preparation method for high purity fish scale collagen

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Experimental program
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Effect test

Embodiment 1

[0018] Take 100 kg of washed fish scales, add 600 liters of 0.5% NaOH solution, and soak for 4 hours. Drain the lye and use it for the alkali soaking process of the next batch of fish scales. Add 800 liters of 0.5% acetic acid solution to the collected fish scale solids, pulverize them with a colloid mill, and grind them into fish scale jelly, that is, fish scale slurry. Adjust the pH with 10% acetic acid solution to achieve a final pH of 3.5. A two-step enzymatic hydrolysis process is adopted. The first step of enzymolysis uses pepsin, the amount of enzyme added is 0.8%, the pH value is adjusted to 3.0, the reaction temperature is 40°C, and the enzymolysis time is 8 hours; the second step of enzymolysis uses high-efficiency protease, and the amount of enzyme added is 0.7 %, adjust the pH value to 4.5, the reaction temperature is 40°C, and the enzymolysis time is 12 hours. The enzymatic hydrolysis product was centrifuged at 5000rpm for 10min, and the supernatant was collect...

Embodiment 2

[0021] Take 100 kg of washed fish scales, add 600 liters of 0.5% NaOH solution, and soak for 4 hours. Drain the lye and use it for the alkali soaking process of the next batch of fish scales. Add 800 liters of 0.5% citric acid solution to the fish scale solids, soak for 4 hours, add a colloid mill, and grind it into a jelly, that is, fish scale slurry. Add 10% citric acid solution to the fish scale slurry, mix thoroughly, and adjust the pH until the final pH reaches 3.5. Add 1.0% protease (pepsin, trypsin, papain, subtilisin or cathepsin, etc.), 35 ℃, under the condition of full stirring, enzymatic hydrolysis for 6h. The enzymatic hydrolysis product was centrifuged at 5000rpm for 10min, and the supernatant was collected, that is, the crude collagen extract.

[0022] Add 10% NaOH solution to the crude collagen extract collected above, and adjust the pH to 3.5. Flow through the cation exchange resin (10×100cm) at a flow rate of 1 times the bed volume / hour, and the volume of t...

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Abstract

The invention provides a new technology for preparing high purity fish scale collagen. The technology uses fish scale as raw material which is soaked in acid base, smashed by a colloid mill and added with protease for enzymolysis; an ion-exchange column chromatographic column separation-membrane separation combined technique is used for separating and purifying the extracted collagen, thus obtaining the collagen with larger than 95% of purity and 1000-5000Da or 100, 000-300, 000Da of molecular weight distribution; and the collagen can be used for preparing health care products, cosmetics and biomedical engineering materials. By adopting the new technology, the waste water production quantity is only 50% of the traditional technology.

Description

technical field [0001] The invention belongs to the technical field of biochemical products, and in particular relates to a production method of high-purity fish scale collagen. Background technique [0002] Collagen, also known as collagen, is mainly distributed in the connective tissue of the human body, and is used as the constituent material of skin, bones, tendons, ligaments, and blood vessels. Most of the raw materials of collagen products currently on the market are extracted from the skins of terrestrial mammals such as pigs and cattle. In recent years, the deterioration of the global ecological environment has led to the occurrence of many epidemics such as mad cow disease and foot-and-mouth disease. Increased potential risk of extracting collagen. Fish collagen is a new type of collagen in recent years. Europe, America, Japan and other countries have successively introduced some restrictions on the extraction of collagen from cattle and other mammals for food, med...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36
Inventor 郭养浩郑允权石贤爱王阿万
Owner FUZHOU UNIV
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