Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for measuring content of Danshensu in shenshuaining capsules by utilizing HPLC method

A technology for Shenshuining and Danshensu, which is applied in the field of determination of the content of Chinese patent medicines, can solve the problem that the quality control mode cannot properly reflect the inherent quality of traditional Chinese medicines, and achieve the effects of good repeatability and stability, convenient use and enhanced controllability.

Active Publication Date: 2010-09-01
云南雷允上理想药业有限公司 +1
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the further deepening of research on traditional Chinese medicine, people gradually realize that for traditional Chinese medicine preparations, the simple quality control mode of Western medicine synthesis can not properly reflect the internal quality of traditional Chinese medicine

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring content of Danshensu in shenshuaining capsules by utilizing HPLC method
  • Method for measuring content of Danshensu in shenshuaining capsules by utilizing HPLC method
  • Method for measuring content of Danshensu in shenshuaining capsules by utilizing HPLC method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] 1. Instruments and reagents

[0016] Instrument: ELIIE-P200II high-performance liquid chromatograph produced by Dalian Yilite Scientific Instrument Co., Ltd., including a variable wavelength ultraviolet detector, chromatographic workstation, and 10 μL quantitative sampling loop.

[0017] Drugs: Shenshuaining Capsules produced by Yunnan Ideal Pharmaceutical Co., Ltd., batch numbers 20090814, 20090921, 20090927;

[0018] Reagents: Danshensu sodium reference substance produced by China Institute of Pharmaceutical and Biological Products, methanol is chromatographically pure, water is pure water, and other reagents are analytically pure.

[0019] 2. Content determination

[0020] 2.1. Chromatographic conditions

[0021] Chromatographic column: Inertsil ODS-3 (4.6X150mm 5μm) filled by Dalian Yili Scientific Instrument Company;

[0022] Mobile phase: methanol: water: the volume ratio of glacial acetic acid is 19:80:1;

[0023] Detection wavelength: 281nm;

[0024] Flow r...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for measuring the content of Danshensu in shenshuaining capsules by utilizing an HPLC method, comprising the following steps: 1, weighing shenshuaining capsules into a measuring flask, crushing, adding methanol, performing ultrasound in water bath, placing in the ice-water bath, taking out, adding methanol, centrifuging, and taking the supernate as the test solution of the shenshuaining capsules; 2, weighing Danshensu sodium comparison products, placing in the measuring flask, performing ultrasound in water bath, taking out, placing in the ice-water bath, taking out, adding methanol to shake up for being served as the comparison products solution; 3, leading the chromatographic column to be Inertsil ODS-3 (4.6X 150mm 5mu m), the mobile phase to be methanol-water-glacial acetic acid, the volume ratio to be 19:80:1, the flow velocity to be 1.0ml / min, the detected wavelength to be 281nm, the sensitivity to be 0.01AUFS, the sample size to be 10 mu L, the column temperature to be 40 DEG C, and the theoretic plate number to be no less than 5000 calculated by chromatographic peak of the Danshensu; and 4, injecting Danshensu sodium comparison products solution and shenshuaining capsules test solution into liquid chromatograph, and measuring the chromatographic peak presented by shenshuaining capsules test solution with the same chromatographic retention as the Danshensu sodium comparison products solution. The invention provides a new comparison standard for accurately assessing the quality of shenshuaining capsules, thus strengthening controllability of product quality of shenshuaining capsules and being beneficial for comprehensive monitoring of product quality of shenshuaining capsules.

Description

technical field [0001] The invention relates to a method for determining the content of Chinese patent medicines by using an HPLC method, in particular to a method for measuring the content of danshensu in Shenshuaining capsules by using the HPLC method. Background technique [0002] Shenshuaining Capsule is a traditional Chinese medicine compound preparation composed of Danshen, Rhubarb, Pseudostellaria, Achyranthes bidentata, Coptis chinensis, Poria cocos, safflower, etc. Among them, Salvia miltiorrhiza is the monarch drug. Salvia miltiorrhiza is the dry root of the Lamiaceae sage plant. Modern pharmacological studies of traditional Chinese medicine have shown that salvia miltiorrhiza can effectively protect the damage to the renal tubular epithelium caused by in situ renal ischemia, and has a significant effect on the serum creatinine concentration after renal ischemia. , creatinine clearance rate and free water clearance rate can be effectively adjusted to a certain degr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/718A61P13/12G01N30/02
Inventor 赵红罗实陈涛李家伟
Owner 云南雷允上理想药业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com