Gene recombinant human Cu-Zn superoxide dismutase liposome enteric-coated capsule and preparation method thereof

Abstract

The invention discloses a method for preparing gene recombinant human superoxide dismutase liposome enteric-coated capsules, which prepares Cu-Zn superoxide dismutase liposome enteric-coated capsules by embedding human Cu-Zn superoxide dismutase (specific activity: 18000U / mg) obtained by the fermentation of engineering bacteria into a liposome. The liposome serving as embedding material adopts the mucosa to absorb the phosphatide and cholesterol of an enhancer, the vitamin E of an antioxidant is added into the liposome, and the enteric-coated capsule which can prevent liposome pharmaceuticals from decomposition and destruction by gastric juice is adopted to avoid the destruction of the medicine, so the gene recombinant human superoxide dismutase liposome enteric-coated capsule can strengthen the absorption of the human Cu-Zn superoxide dismutase, improve the blood concentrations and pesticide effect, remove the free radical from human body better, and delay the process of aging. The encapsulation ratio of the human Cu-Zn superoxide dismutase (Cu-Zn SOD) liposome is 80.54 + / - 2.26 percent, and the tolerances of the enteric-coated capsule under the simulated condition of gastric acid, pepsin and pancreatic enzyme in vitro are respectively 95.2 percent, 91.6 percent and 86.2 percent.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to genetically recombinant human copper-zinc superoxide dismutase (Cu-ZnSOD) liposome enteric-coated capsules and a preparation method thereof, in particular to lipids of recombinant human copper-zinc superoxide dismutase Body embedding technology and preparation method of enteric-coated capsules. Background technique [0002] At present, it is believed that free radicals (also known as free radicals) are related to most diseases and the aging of the human body. The so-called free radical is that when the body is metabolizing, it can take away an electron from oxygen, so that the oxygen atom becomes a free radical. Free radicals are very unstable. They need to grab electrons from the molecules of body tissue cells to pair themselves, so that the molecules that have been stripped of electrons also become free radicals. There are many kinds of free radicals, such as superoxide ani...

AI Technical Summary

Problems solved by technology

The superoxide dismutase produced by yeast itself is regulated by its own mechanism and is limited to a narrow range; there are many impurities in the intracellular extraction, the process is complicated, the enzyme loss is large during the extraction process, and the enzyme activity is also greatly affected

There are fewer research reports on the production of SOD by genetic engineering fermentation, such as the application number 97103939.9 "Recombinant Human Copper-Zinc Superoxide Dismutase Preparation Process", the recombinant human copper-zinc superoxide dismutase expressed by engineering bacteria is in the form of inclusion bodies Existence, during the purification process, ultrasonic disruption of the bacteria and washing with organic solvents will cause a large amount of target protein loss, and the renaturation is also difficult, and the enzyme activity is low

There is no report on the production of recombinant human copper-zinc SOD by genetic engineering technology, and the preparation of recombinant human copper-zinc superoxide dismutase (Cu-Zn SOD) liposome enteric capsules by liposome embedding technology.