Gene recombinant human Cu-Zn superoxide dismutase liposome enteric-coated capsule and preparation method thereof

A technology of dismutase lipid and superoxide, which is applied in the fields of botanical equipment and methods, biochemical equipment and methods, and capsule transportation, etc., can solve the problems of complex process, many impurities in extraction, loss of target protein, etc.

Inactive Publication Date: 2010-09-01
江西宇骏生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The superoxide dismutase produced by yeast itself is regulated by its own mechanism and is limited to a narrow range; there are many impurities in the intracellular extraction, the process is complicated, the enzyme loss is large during the extraction process, and the enzyme activity is also greatly affected
There are fewer research reports on the production of SOD by genetic engineering fermentation, such as the application number 97103939.9 "Recombinant Human Copper-Zinc Superoxide Dismutase Preparation Process", the recombinant human copper-zinc superoxide di

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Strain screening and amplification: select a single colony of positive clones on the MD plate, inoculate it in 60ml YPD medium in a sterile environment, and activate it on a shaker at 30°C and 250rpm for 10 hours; the activated strains are inoculated at 10% The amount was respectively inoculated into three 500ml Erlenmeyer flasks containing 200ml of YPD medium, and activated at 30°C and 250rpm for 10 hours. Amplify 600ml strains according to the above fermentation conditions and fermentation time.

[0042] (2) Fermentation culture: Inoculate 500ml strains in a 10L fermenter with a loading capacity of 5L, the fermentation medium is BMGY medium, and the fermentation conditions are: rotation speed 400rpm; pH 5.0; temperature: 30°C; dissolved oxygen 40 % above; culture time 16 hours. After culturing for 16 hours, the medium was fed, and the feeding was divided into two stages. The first stage of the feeding medium was 2×BMMY+6% methanol; pH 5.3; the feeding volume was ...

Embodiment 2

[0052] (1) Strain screening and amplification: select a single colony of positive clones on the MD plate, inoculate it in 60ml YPD medium in a sterile environment, and activate it on a shaker at 30°C and 250rpm for 10 hours; the activated strains are inoculated at 10% The amount was respectively inoculated into three 500ml Erlenmeyer flasks containing 200ml of YPD medium, and activated at 30°C and 250rpm for 10 hours. Amplify 600ml strains according to the above fermentation conditions and fermentation time.

[0053] (2) Fermentation culture: Inoculate 500ml strains in a 10L fermenter with a loading capacity of 5L, the fermentation medium is BMGY medium, and the fermentation conditions are: rotation speed 400rpm; pH 5.5; temperature: 30°C; dissolved oxygen 40 % above; culture time 16 hours. After culturing for 16 hours, the medium was fed, and the feeding was divided into two stages. The first stage of the feeding medium was 2×BMMY+6% methanol; pH 5.3; the feeding volume was ...

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Abstract

The invention discloses a method for preparing gene recombinant human superoxide dismutase liposome enteric-coated capsules, which prepares Cu-Zn superoxide dismutase liposome enteric-coated capsules by embedding human Cu-Zn superoxide dismutase (specific activity: 18000U/mg) obtained by the fermentation of engineering bacteria into a liposome. The liposome serving as embedding material adopts the mucosa to absorb the phosphatide and cholesterol of an enhancer, the vitamin E of an antioxidant is added into the liposome, and the enteric-coated capsule which can prevent liposome pharmaceuticals from decomposition and destruction by gastric juice is adopted to avoid the destruction of the medicine, so the gene recombinant human superoxide dismutase liposome enteric-coated capsule can strengthen the absorption of the human Cu-Zn superoxide dismutase, improve the blood concentrations and pesticide effect, remove the free radical from human body better, and delay the process of aging. The encapsulation ratio of the human Cu-Zn superoxide dismutase (Cu-Zn SOD) liposome is 80.54 +/- 2.26 percent, and the tolerances of the enteric-coated capsule under the simulated condition of gastric acid, pepsin and pancreatic enzyme in vitro are respectively 95.2 percent, 91.6 percent and 86.2 percent.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to genetically recombinant human copper-zinc superoxide dismutase (Cu-ZnSOD) liposome enteric-coated capsules and a preparation method thereof, in particular to lipids of recombinant human copper-zinc superoxide dismutase Body embedding technology and preparation method of enteric-coated capsules. Background technique [0002] At present, it is believed that free radicals (also known as free radicals) are related to most diseases and the aging of the human body. The so-called free radical is that when the body is metabolizing, it can take away an electron from oxygen, so that the oxygen atom becomes a free radical. Free radicals are very unstable. They need to grab electrons from the molecules of body tissue cells to pair themselves, so that the molecules that have been stripped of electrons also become free radicals. There are many kinds of free radicals, such as superoxide ani...

Claims

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Application Information

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IPC IPC(8): A61K38/44A61K9/48A61K47/34A61P39/06C12N15/53C12N15/81C12N9/08A61K47/24
Inventor 蔡刚梁华正黄建荣楼秀余赖建炫
Owner 江西宇骏生物工程有限公司
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