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Application of scallop polysaccharide in preparation of anti-herpes simplex virus medicament

A technology of herpes simplex virus and scallop polysaccharide, applied in the field of medicine, can solve the problems of host cell damage and cross-resistance

Inactive Publication Date: 2010-09-15
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, only a few drugs such as herpesnet, acyclovir, and vidarabine are recognized and used clinically at home and abroad, because their drug targets are all DNA synthetases, and cross-resistance often occurs
At the same time, because viruses have the characteristics of intracellular parasitism and dependence on host cell functions during replication, drugs often cause damage to normal host cells while killing virus-infected cells, causing various adverse reactions

Method used

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  • Application of scallop polysaccharide in preparation of anti-herpes simplex virus medicament
  • Application of scallop polysaccharide in preparation of anti-herpes simplex virus medicament
  • Application of scallop polysaccharide in preparation of anti-herpes simplex virus medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Preparation of anti-herpes simplex virus preparation

[0017] Use distilled water to make a scallop polysaccharide solution containing 5% by mass, add 1% by mass of activated carbon to stir, heat at 80°C, centrifuge, collect the supernatant, and discard the precipitate; suction filter the supernatant, Further remove activated carbon and its adsorbed proteins and pigments to obtain a clear liquid; apply ultrafiltration to the clear liquid with 100k and 5k filter membranes successively to obtain a filtrate with a molecular weight of 5k to 100k; freeze-dry the filtrate to obtain a light yellow powder, Purified scallop polysaccharide with a molecular weight of 5k-100k. Grind the purified scallop polysaccharide through an 80-mesh sieve, mix it with medicinal starch in a weight ratio of 1:1-10, and prepare capsules with specifications of 50 mg or 100 mg. Or grind the purified scallop polysaccharide through an 80-mesh sieve, and make tablets of 50 mg or 100 mg a...

Embodiment 2

[0031] Example 2: In vitro anti-herpes simplex virus type I effect of scallop polysaccharide: in vitro anti-herpes simplex virus type I experiment using African green monkey kidney cells as host cells

[0032] 1 material

[0033] Scallop polysaccharide (GAG): The powder provided by the scallop polysaccharide research group of the Pharmacology Teaching and Research Office of Qingdao University School of Medicine. Serum-free DMEM medium was used to make solutions with different concentrations during the experiment.

[0034] African green monkey kidney cells (Vero cells), donated by the Animal Quarantine Institute of the Ministry of Agriculture, added 10% newborn bovine serum, 100U / ml penicillin, 100μg / ml streptomycin, and placed in 5% CO 2 Cultured at 37°C in an incubator. Passage every three days.

[0035] Standard herpes simplex virus type I (HSV-I) SM 44 Strains provided by Peking Union Medical College. The Vero cells were subcultured three times and then centrifuged (300...

Embodiment 3

[0085] Example 3: In vitro anti-herpes simplex virus type I effect of scallop polysaccharide: In vitro anti-herpes simplex virus experiment using human cervical cancer cells (Hela cells) as host cells

[0086] 1 material

[0087] 1.1 medicine and virus: with embodiment 2.

[0088] 1.2-cell human cervical cancer cells (Hela cells), donated by the Department of Microbiology, Qingdao University.

[0089] 2 The experimental method is the same as in Example 2.

[0090] 3 results

[0091] 3.1 Drug toxicity test results

[0092] The morphology and proliferative activity of Hela cells treated with scallop polysaccharide (50-800 μg / ml) had no significant difference from those of the blank control group (P>0.05). It shows that scallop polysaccharide has no toxic effect on Hela cells in the range of 50-800μg / ml. See Table 1.5.

[0093] Table 1.5 The effect of different concentrations of scallop polysaccharide on the proliferation activity of Hela cells ( n=6)

[0094]

[0095...

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Abstract

The invention relates to application of scallop polysaccharide in the preparation of an anti-herpes simplex virus medicament, which comprises the following steps of: preparing 5 mass percent solution of scallop polysaccharide by using distilled water, absorbing the solution of scallop polysaccharide through an active carbon, and heating the solution; centrifuging and filtering the solution; performing ultrafiltration on the filtrate by using 100k and 5k filter membranes sequentially; performing the freeze drying of the filtrate to obtain the light yellow powder-type purified scallop polysaccharide with a molecular weight of between 5 and 100k; and grinding the purified scallop polysaccharide and screening the ground scallop polysaccharide with an 80 mesh screen, and uniformly mixing the ground scallop polysaccharide with a medical starch in the weight ratio of 1:1-10 to prepare 50mg or 100mg of capsules; or grinding the purified scallop polysaccharide and screening the ground scallop polysaccharide with an 80 mesh screen to prepare 50mg or 100mg of tablets by a wet palletizing process. The scallop polysaccharide is the natural product, and has the advantages of low toxicity, light side effect, high safety and excellent anti-herpes simplex virus effect.

Description

Technical field: [0001] The invention relates to the application of scallop polysaccharide in the preparation of anti-herpes simplex virus medicine, which belongs to the technical field of medicine. Background technique: [0002] Herpes simplex virus (HSV) is a common human infectious disease virus, mainly infecting the skin and mucous membranes of the mouth, eyes, lips, central nervous system, genitals, internal organs, etc., causing herpes labialis, encephalitis, keratoconjunctivitis, genital diseases such as herpes. Especially after pregnant women are infected with HSV, they are prone to miscarriage, resulting in fetal congenital deformities and mental retardation. About 40% to 60% of newborns will develop high fever, dyspnea and central nervous system lesions after being infected by HSV-2 through the birth canal, of which 60% % to 70% of infected newborns may die as a result, and the sequelae of survivors can reach 95%. It has been confirmed that HSV infection is relat...

Claims

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Application Information

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IPC IPC(8): A61K31/737A61P31/22
Inventor 刘赛鞠传霞
Owner QINGDAO UNIV
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