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Method for preparing calcium carbonate microspheres

A technology of calcium carbonate and microspheres, applied in the field of materials, can solve the problems of inability to effectively regulate the structure and morphology of calcium carbonate, regulation of silk fibroin nanostructures, unsuitable for large-scale production, etc., achieving easy control, simple preparation method, Size controllable effect

Inactive Publication Date: 2010-09-29
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Kaplan DL et al. found in the literature "Selective in vitro effect of peptides on calcium carbonate crystallization" published in "Crystal Growth and Design" in 2002 that using peptides as templates can regulate the nano- and micro-structure and morphology of calcium carbonate, but its use Synthesizing specific polymers or peptides as templates is expensive and not suitable for large-scale production
At present, it can be seen that silk fibroin is used as a template to prepare literature and patent reports of calcium carbonate powder (as the application number is the patent "silk fibroin / calcium carbonate nanocomposite material and its preparation method" of CN200710069129.8, An XQ etc. in 2008 "Coeffect of silk fibroin and self-assembly monolayers on the biomineralization of calcium carbonate" published in "Journal of Physical Chemistry C", and "Silk fibroin-regulated crystallization of calcium carbonate”), but none of them can regulate the nanostructure of silk fibroin, so they cannot effectively regulate the structure and morphology of calcium carbonate

Method used

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  • Method for preparing calcium carbonate microspheres
  • Method for preparing calcium carbonate microspheres
  • Method for preparing calcium carbonate microspheres

Examples

Experimental program
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Effect test

Embodiment 1

[0028] The silk in 0.5% Na 2 CO 3 Cook in the solution at 100°C for 60 minutes to remove sericin on the outside of the silk, rinse with deionized water, repeat 3 times, and dry at 60°C to obtain pure silk fibroin. Dissolve 1 g of silk fibroin in 10 mL of ternary solution (by CaCl 2 / water / ethanol molar ratio is 1: 8: 2), and this silk fibroin solution is packed in dialysis bags and immersed in deionized water for dialysis for 4 days, during which the water is changed every two hours to remove CaCl in the solution 2 And ethanol, thereby obtain pure silk fibroin solution, its concentration is 2%.

[0029] Concentrate the solution to 10%, incubate at 50°C for 6 hours, dilute it back to 0.8%, take 15mL and put it in a container with electrodes, turn on the power supply and apply a voltage of 50V to build a field strength of 3×10 3 Oriented electric field of V / m, energized for 5 minutes, silk fibroin self-assembles under the action of electric field to form silk fibroin nanop...

Embodiment 2

[0032] The silk in 0.5% Na 2 CO 3 Cook in the solution at 100°C for 60 minutes to remove sericin on the outside of the silk, rinse with deionized water, repeat 3 times, and dry at 60°C to obtain pure silk fibroin. 1g of silk fibroin was dissolved in 10mL ternary solution (by CaCl 2 / water / ethanol molar ratio is 1: 8: 2), and this silk fibroin solution is packed in dialysis bags and immersed in deionized water for dialysis for 4 days, during which the water is changed every two hours to remove CaCl in the solution 2 And ethanol, thereby obtain pure silk fibroin solution, its concentration is 2%.

[0033] Concentrate the solution to 10%, incubate at 70°C for 6 hours, dilute it back to 2% concentration, take 15mL and put it in a container with electrodes, turn on the power supply and apply a voltage of 20V, and build a field strength of 1×10 3 A directional electric field of V / m is applied for 30 minutes to form a gel-like substance, which is freeze-dried at a temperature o...

Embodiment 3

[0036] The silk in 0.5% Na 2 CO 3 Cook in the solution at 100°C for 60 minutes to remove sericin on the outside of the silk, rinse with deionized water, repeat 3 times, and dry at 60°C to obtain pure silk fibroin. 1g of silk fibroin was dissolved in 10mL ternary solution (by CaCl 2 / water / ethanol molar ratio is 1: 8: 2), and this silk fibroin solution is packed in dialysis bags and immersed in deionized water for dialysis for 4 days, during which the water is changed every two hours to remove CaCl in the solution 2 0 and ethanol, thereby obtaining pure silk fibroin solution, its concentration is 2%.

[0037] The solution was sealed and incubated at 40°C for 36 hours, 15mL was taken and placed in a container with electrodes installed, the power was turned on and a voltage of 35V was applied to build a field strength of 3.5×10 3 V / m directional electric field, electrified for 5 minutes, silk fibroin self-assembles under the action of electric field to form silk fibroin nan...

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Abstract

The invention belongs to the field of materials, and in particular relates to a method for preparing calcium carbonate microspheres by using fibroin nanospheres as a template. The method comprises the following steps of: preparing 0.1 to 20 mass percent aqueous solution of silk fibroin and hermetically culturing the aqueous solution of silk fibroin for 0.5 to 48 hours at the temperature of between 10 and 90 DEG C; performing self-assembly on the silk fibroin when the electric intensity is 1.0*10<2>-1.0*10<5>V / m to form nanometer gel particles with the diameter of 10 to 2,000nm; performing freeze drying on the nanometer gel particles at the temperature of between 80 below zero and 0 DEG C to obtain solid silk fibroin nanospheres with the diameter of 10 to 2,000nm, wherein the nanometer gel particles or the solid silk fibroin nanospheres are used as the template; and forming a calcium carbonate shell on the surface of the template to prepare the calcium carbonate microspheres. The whole preparation process is performed at normal temperature and normal pressure without adding any toxic chemical reagent; and the calcium carbonate microspheres have controllable size and can fully meet the application requirements of various different fields such as cosmetic, ink, coatings, medicament-carrying materials and the like.

Description

technical field [0001] The invention belongs to the field of materials, and in particular relates to a method for preparing calcium carbonate microspheres by using silk fibroin nanospheres as templates. Background technique [0002] Silk fibroin is a structural protein that exists widely in nature. It can be extracted from silk fiber of silkworm. It has sufficient sources, simple extraction method, good biocompatibility, biodegradability, and excellent mechanical properties. Adhesion, amplification, and differentiation are good, and it is a promising organic matrix material. Silk fibroin can be prepared into various forms such as solutions, films, porous scaffolds, gels, etc., and extensive research has been carried out in the field of biomedicine. [0003] As the most extensive mineral in the world, calcium carbonate has three homogeneous anomalies: calcite (rhomboid), aragonite (acicular), and vaterite (spherical). In organisms, due to the special combination of calcium ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01F11/18
Inventor 黄永利吕强卢神州李明忠王建南
Owner SUZHOU UNIV
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