Emulsion core coaxial electrically spun ultrafine fibrous membrane with clad proteins and preparation method thereof

A technology of coaxial electrospinning and ultrafine fibers, which is applied in the direction of spinning solution preparation, cellulose/protein conjugated artificial filament, fiber treatment, etc. It can solve the problems of protein activity influence, too little release during operation, etc. To achieve the effect of slowing down the burst release

Inactive Publication Date: 2010-11-24
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the drug-loaded core/shell fibers prepared by coaxial or emulsion electrospinning generally have a serious burst release phenomenon in the early release period (within 1 day), and the release amount during the operation period is too small (Li H, Zhao C G, Wang Z X, et al. "Controlled release of PDGF-bb by coaxial electrospun dextran/poly(L-lactide-co-ε-caprolactone) fibers with an ultrafine core/shell structure", Jour

Method used

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  • Emulsion core coaxial electrically spun ultrafine fibrous membrane with clad proteins and preparation method thereof
  • Emulsion core coaxial electrically spun ultrafine fibrous membrane with clad proteins and preparation method thereof

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Embodiment 1

[0013] 1. Preparation of each component of the W / O emulsion: Phase 0 is a solution obtained by dissolving PLGA (ethylene glycol as an initiator, LA:GA=3:1) with a relative molecular mass of 30,000 in chloroform, and phase W is BSA in water.

[0014] 2. Inner core solution: After adding the W phase prepared in step 1 to the O phase, oscillate with a vortex mixer for 5 minutes to prepare a W / O emulsion as the inner core solution.

[0015] 3. Shell solution: 100mg / mL PELCL (PELCL is a ring-opening polymerization method, specifically: polymerized monomers: lactide (LA) and caprolactone (CL), react at 140°C for 24 hours in an anhydrous and oxygen-free environment Hours, stannous octoate as catalyst, polyethylene glycol 2000 as initiator, LA: CL=3: 1; molecular weight is 120,000) solution, solvent is the mixed solvent of chloroform and trifluoroethanol with volume ratio 7: 3.

[0016] 4. Electrospin the inner core solution and the outer shell solution through a coaxial electrospinn...

Embodiment 2

[0018] 1. The preparation of each component of the W / O emulsion: the O phase is a solution obtained by dissolving PELGA (polyethylene glycol 2000 as an initiator, LA:GA=3:1) with a relative molecular weight of 30,000 in chloroform, W The phase is BSA in water.

[0019] 2. Inner core solution: After adding the W phase prepared in step 1 to the O phase, oscillate with a vortex mixer for 5 minutes to prepare a W / O emulsion as the inner core solution.

[0020] 3. Shell solution: 100mg / mL PELCL (preparation method is the same as above, LA:CL=3:1, molecular weight is 120,000) solution, the solvent is a mixed solvent of chloroform and trifluoroethanol with a volume ratio of 7:3.

[0021] 4. Electrospin the inner core solution and the outer shell solution through a coaxial electrospinning device. Each solution (emulsion and shell) is output from the syringe at a specific flow rate through an independent micro-syringe pump. The electrospinning voltage output by the high-voltage DC po...

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Abstract

The invention relates to a method for preparing an emulsion core coaxial electrically spun ultrafine fibrous membrane with clad proteins, which comprises the following step of electrospinning by using a device the same with coaxial electrospinning by using an emulsion containing bovine serum albumin (BSA) as an inner core solution and using a polyethylene glycol-co-poly(lactide-co-caprolactone) solution as a shell solution. The preparation method of the emulsion comprises following steps of: using polyethylene glycol-co-poly(lactide-co-caprolactone) or the chloroformic solution of the polyethylene glycol-co-poly(lactide-co-caprolactone) as an oil phase, using 300-500 mg/mL of BAS aqueous solution as a water phase, adding the water phase into the oil phase and oscillating by a cyclone mixer for 5 min to prepare the emulsion. The electrospinning voltage is 16 kV; the reception distance is 20 cm; and the flow rate of the inner core solution is 0.05-0.1 mL/h. The cladding of the BSA in the fiber is determined by a transmission electron microscope. The electrically spun fiber prepared by using the method is also applicable for the cladding and the release of other proteins.

Description

technical field [0001] The invention relates to a milk core-coaxial electrospun superfine fiber membrane loaded with protein and a preparation method thereof, which belongs to the electrostatic spinning preparation technology capable of loading and releasing protein. Background technique [0002] Electrospun ultrafine fiber membranes have a fiber morphology similar to natural extracellular matrix, so they are widely used in tissue engineering scaffolds to provide support for cell growth and correspondingly required mechanical properties. Loading biologically active substances in fibers can promote and guide the growth, reproduction and differentiation of cells, which is beneficial to the formation of tissues and the improvement of functions. (Pham Q P, Sharma U, Mikos A G, "Electrospinning of polymeric nanofibers for tissue engineering applications: a review", Tissue Engineering, 2006, 12(5): 1197-1211; Sill T J, von Recum H A, "Electrospinning: Applications in drug deliver...

Claims

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Application Information

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IPC IPC(8): D01F8/02D01F8/14D01D1/02D01D5/00A61L27/22A61L27/18A61L27/54
Inventor 袁晓燕张红
Owner TIANJIN UNIV
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