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Centrifugal ultrafiltration device suitable to be used in preprocessing of micro sample

A sample pretreatment and trace technology, applied in the preparation of test samples, semi-permeable membrane separation, chemical instruments and methods, etc., can solve the problems of ultrafiltration membrane filterability change, membrane seepage flow decrease, separation efficiency decrease, etc. Achieve the effect of prolonging service life, simple application, and speeding up ultrafiltration

Inactive Publication Date: 2010-12-01
HEBEI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the ultrafiltration centrifuge tube with this structure is prone to concentration polarization during the centrifugal ultrafiltration process, which indirectly leads to a decrease in membrane permeation and a decrease in separation efficiency, and is only suitable for the concentration and preparation of cells and macromolecules
In order to solve the problem of concentration polarization, researchers tilted the ultrafiltration membrane at a certain angle in order to improve the phenomenon of concentration polarization, but the effect was not obvious
Moreover, under the action of a large centrifugal force, the filterability of the ultrafiltration membrane will change, or even rupture.

Method used

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  • Centrifugal ultrafiltration device suitable to be used in preprocessing of micro sample
  • Centrifugal ultrafiltration device suitable to be used in preprocessing of micro sample
  • Centrifugal ultrafiltration device suitable to be used in preprocessing of micro sample

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Effect test

Embodiment 1

[0045] Centrifugal ultrafiltration device of the present invention, its structure can refer to figure 1 As shown, a centrifuge tube 1 and a plug cover 3 are included. Centrifuge tube 1 can adopt the tubular container that materials such as plastics, glass, stainless steel are made. Plug cover 3 can adopt rubber stopper, cork etc. A hollow fiber membrane 2 parallel to the axis of the centrifuge tube is arranged in the centrifuge tube 1 . In actual application, the hollow fiber membrane 2 with a suitable molecular weight cut-off can be selected according to the characteristics of the sample to be processed. The outlet end of the hollow fiber membrane 2 is connected to the outside of the centrifuge tube 1 through the plug cap 3 . In this way, the sample can be placed in the centrifuge tube 1 in use, and the hollow fiber membrane 2 is immersed therein. When the centrifuge rotates, under the action of centrifugal force, molecules of appropriate size and solvents enter the centr...

Embodiment 2

[0051] For the sample processing method containing polymer stabilizers in the analysis of pharmaceutical preparations:

[0052] Take the microecological preparation containing polymer xanthan gum-"sucrose gel", accurately weigh 0.1 g of sucrose, put it in a 10 mL measuring bottle, add 5 mL of deionized water to dissolve, vortex for 3 minutes, and add water to dilute to the mark. Put the above solution into a centrifuge tube, insert two injection needles through the rubber stopper into the processed U-shaped hollow fiber membrane with a molecular weight cut off of 6000, and put it into the centrifuge tube. Centrifuge in a centrifuge for 20 minutes at 3500 rpm, take out the hollow fiber membrane, use a sampler to extract the test solution in the hollow fiber membrane, and inject 10 μL into the chromatographic column for analysis.

Embodiment 3

[0057] Sample handling methods for in vivo drug analysis:

[0058] Precisely measure 225 μL of plasma into a narrow glass centrifuge tube (inner diameter is about 5 mm) (see image 3 ), add 25 μL of internal standard cephradine solution, vortex and mix for 1 min, bend the hollow fiber membrane with a molecular weight cut-off of 6000 activated in water into a U shape and place it in the tube (making both ends of the hollow fiber membrane above the liquid level of the plasma ), centrifuge (3500 rpm) for 30min, take out the hollow fiber membrane, extract 20 μL of the solution therefrom for HPLC analysis, and record the chromatogram.

[0059] Analytical chromatograms such as Figure 4 shown.

[0060] The analysis results show that the device can quickly separate high-molecular substances such as proteins from small-molecular substances in biological samples, eliminating the need for complicated sample pretreatment processes (precipitation of proteins, centrifugal filtration, ext...

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Abstract

The invention discloses a centrifugal ultrafiltration device suitable to be used in the preprocessing of a micro sample, which comprises a centrifuge tube, and is characterized in that a hollow fiber film which is parallel to the axis of the centrifuge tube and of which the outlet extends out of the centrifuge tube are arranged in the centrifuge tube. The centrifugal ultrafiltration device can bewidely used in the preprocessing of the micro sample as well as the separation, purification and concentration of micro biological samples. The use of the device is simple, quick and convenient; the processed sample suffers little interference and has high sensitivity; and, the service life of a chromatographic column is prolonged effectively.

Description

Technical field: [0001] The invention relates to a centrifugal ultrafiltration device, in particular to a centrifugal ultrafiltration device suitable for micro sample pretreatment. Background technique [0002] Sample preparation is one of the most important processes in analytical chemistry. Usually, the time required for each sample pretreatment accounts for two-thirds of the whole analysis process, and the error caused by sample pretreatment accounts for one third of the analysis results. Therefore, the development of advanced sample pretreatment technology and sample pretreatment device is a hot and difficult point in modern analysis technology. [0003] The current sample pretreatment usually includes sample preparation and sample separation and enrichment. With the advancement of modern analysis technology, the combination of chromatography, spectroscopy and mass spectrometry has become the main development direction of separation and enrichment technology. Correspo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D61/00B01D63/16G01N1/28
Inventor 蒋晔康丽娟李珺沬孙婷任淑萌李岑安静
Owner HEBEI MEDICAL UNIVERSITY
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