Method for purifying aquatic water by using microbes and plants in combination
A technology for aquaculture water and microbial cultivation, which is applied in the field of joint purification of aquaculture water by using microorganisms and plants, can solve the problems of inability to remove, easy to lose, and short duration, and achieve no secondary pollution, high nitrogen and phosphorus absorption capacity, and fast The effect of growth rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0027] A method of utilizing microorganisms and plants to jointly purify aquaculture water of the present invention comprises the following steps:
[0028] (1) Immobilized microbial flora:
[0029] a. Use ammonia oxidation medium to screen out three non-pathogenic strains with high-efficiency denitrification effects such as heterotrophic nitrification and aerobic denitrification from aquaculture water or aquaculture sludge: Bacillus megaterium, bent Bacillus flexus and Bacillus cereus;
[0030] b. Inject Bacillus megaterium, Bacillus flexus and Bacillus cereus into beef extract peptone liquid medium respectively, culture at 30°C for 18 hours, and then centrifuge at 8000rpm for 10min , the solid precipitate was resuspended twice with saline to obtain OD 600 Bacterial suspension with a value of 0.8;
[0031] c. Mix the bacterial suspensions in an equal volume ratio to obtain the microbial flora, insert 1% of the inoculum into the ammonia oxidation medium to obtain the mixed b...
Embodiment 2
[0041] A method of utilizing microorganisms and plants to jointly purify aquaculture water of the present invention comprises the following steps:
[0042] (1) Immobilized microbial flora:
[0043]a. Use the denitrification medium to screen out three non-pathogenic strains with high-efficiency denitrification effects such as heterotrophic nitrification and aerobic denitrification from the aquaculture water or aquaculture sludge: Bacillus megaterium, bent Bacillus flexus and Enterobacter aerogenes;
[0044] b. Inject Bacillus megaterium, Bacillus flexus and Enterobacter aerogenes into beef extract peptone liquid medium respectively, culture at 37°C for 24 hours, and centrifuge at 10,000 rpm for 8 minutes , the obtained solid precipitate was resuspended 3 times with normal saline, and the OD 600 Bacterial suspension with a value of 1;
[0045] c. The bacterial suspension of Bacillus megaterium (Bacillus megaterium), the bacterial suspension of Bacillus flexus (Bacillus flexus...
Embodiment 3
[0055] A method of utilizing microorganisms and plants to jointly purify aquaculture water of the present invention comprises the following steps:
[0056] (1) Immobilized microbial flora:
[0057] a. Two strains of non-pathogenic bacteria with high-efficiency denitrification are screened from the aquaculture water body or aquaculture sludge by using the ammonia oxidation medium: Bacillus flexus and Bacillus cereus;
[0058] b. Bacillus flexus (Bacillus flexus) and Bacillus cereus (Bacillus cereus) were inserted into beef extract peptone liquid medium respectively, after culturing at 35°C for 20 hours, centrifuged at 12000rpm for 5min, and the solid precipitate obtained after centrifugation Resuspended twice with normal saline to obtain OD 600 Bacterial suspension with a value of 1.5;
[0059] c. Mix the bacterial suspension of Bacillus flexus and the bacterial suspension of Bacillus cereus at a volume ratio of 2:1 to obtain microbial flora, and insert ammonia oxidation with...
PUM
Property | Measurement | Unit |
---|---|---|
pore size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com