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Method for purifying aquatic water by using microbes and plants in combination

A technology for aquaculture water and microbial cultivation, which is applied in the field of joint purification of aquaculture water by using microorganisms and plants, can solve the problems of inability to remove, easy to lose, and short duration, and achieve no secondary pollution, high nitrogen and phosphorus absorption capacity, and fast The effect of growth rate

Inactive Publication Date: 2010-12-01
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, domestic aquaculture water purification methods are mainly to convert aquaculture wastewater through biofilm nitrification tanks to convert organic matter and ammonia nitrogen into relatively low-toxic nitrates, which can improve water quality to a certain extent, but this method can lead to Nitrate accumulates in a large amount in aquaculture water, and at the same time, it cannot remove other nutrients such as organic particles and inorganic phosphorus in aquaculture water. Therefore, in order to overcome the shortcomings of purifying aquaculture water through biofilm nitrification tanks, microorganisms and plants are usually used for joint purification. Aquaculture water body
[0003] The existing method of using microorganisms and plants to jointly purify aquaculture water is mainly to cultivate plants in the form of floating islands in the aquaculture water body. The free microorganisms in the roots of the plants can effectively decompose the organic matter and ammonia nitrogen in the water body into nitrate, and decompose to obtain Nutrients such as nitrate and inorganic phosphorus are absorbed by the roots of plants, but the free microorganisms in the roots of plants have the disadvantages of easy death, loss, low activity, and low degradation efficiency, resulting in low purification efficiency and short duration of aquaculture water. question

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method of utilizing microorganisms and plants to jointly purify aquaculture water of the present invention comprises the following steps:

[0028] (1) Immobilized microbial flora:

[0029] a. Use ammonia oxidation medium to screen out three non-pathogenic strains with high-efficiency denitrification effects such as heterotrophic nitrification and aerobic denitrification from aquaculture water or aquaculture sludge: Bacillus megaterium, bent Bacillus flexus and Bacillus cereus;

[0030] b. Inject Bacillus megaterium, Bacillus flexus and Bacillus cereus into beef extract peptone liquid medium respectively, culture at 30°C for 18 hours, and then centrifuge at 8000rpm for 10min , the solid precipitate was resuspended twice with saline to obtain OD 600 Bacterial suspension with a value of 0.8;

[0031] c. Mix the bacterial suspensions in an equal volume ratio to obtain the microbial flora, insert 1% of the inoculum into the ammonia oxidation medium to obtain the mixed b...

Embodiment 2

[0041] A method of utilizing microorganisms and plants to jointly purify aquaculture water of the present invention comprises the following steps:

[0042] (1) Immobilized microbial flora:

[0043]a. Use the denitrification medium to screen out three non-pathogenic strains with high-efficiency denitrification effects such as heterotrophic nitrification and aerobic denitrification from the aquaculture water or aquaculture sludge: Bacillus megaterium, bent Bacillus flexus and Enterobacter aerogenes;

[0044] b. Inject Bacillus megaterium, Bacillus flexus and Enterobacter aerogenes into beef extract peptone liquid medium respectively, culture at 37°C for 24 hours, and centrifuge at 10,000 rpm for 8 minutes , the obtained solid precipitate was resuspended 3 times with normal saline, and the OD 600 Bacterial suspension with a value of 1;

[0045] c. The bacterial suspension of Bacillus megaterium (Bacillus megaterium), the bacterial suspension of Bacillus flexus (Bacillus flexus...

Embodiment 3

[0055] A method of utilizing microorganisms and plants to jointly purify aquaculture water of the present invention comprises the following steps:

[0056] (1) Immobilized microbial flora:

[0057] a. Two strains of non-pathogenic bacteria with high-efficiency denitrification are screened from the aquaculture water body or aquaculture sludge by using the ammonia oxidation medium: Bacillus flexus and Bacillus cereus;

[0058] b. Bacillus flexus (Bacillus flexus) and Bacillus cereus (Bacillus cereus) were inserted into beef extract peptone liquid medium respectively, after culturing at 35°C for 20 hours, centrifuged at 12000rpm for 5min, and the solid precipitate obtained after centrifugation Resuspended twice with normal saline to obtain OD 600 Bacterial suspension with a value of 1.5;

[0059] c. Mix the bacterial suspension of Bacillus flexus and the bacterial suspension of Bacillus cereus at a volume ratio of 2:1 to obtain microbial flora, and insert ammonia oxidation with...

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Abstract

The invention discloses a method for purifying aquatic water by using microbes and plants in combination, which is characterized by comprising the following steps: (1), screening at least two kinds of avirulent microbes capable of denitrifying efficiently from the aquatic water or aquatic sludge by using an ammoxidation culture medium or a denitrification culture medium, mixing the two kinds of avirulent microbes, immobilizing the obtained microbial colonies on a carrier to complete the immobilization of the microbial colonies; (2) building a plant filter pond communicated with an aquatic pond, planting edible vegetables or forage grasses in the plant filter pond, filling the immobilizing carrier on which the microbial colonies are immobilized in a plastic mesh bag and putting the plastic mesh bag at the root of plants; and (3) building a closed circulation aquatic system and driving aquatic water circulation. The method has the advantages of overcoming the drawbacks of easy death and loss of floating microbes, low activity and low degradation efficiency, improving the purification efficiency and reducing the purification time of the aquatic water by using the synergistic effect between the plants and the microbes, and saving water source.

Description

technical field [0001] The invention relates to a method for purifying aquaculture water, in particular to a method for jointly purifying aquaculture water by using microorganisms and plants. Background technique [0002] At present, domestic aquaculture water purification methods are mainly to convert aquaculture wastewater through biofilm nitrification tanks to convert organic matter and ammonia nitrogen into relatively low-toxic nitrates, which can improve water quality to a certain extent, but this method can lead to Nitrate accumulates in a large amount in aquaculture water, and at the same time, it cannot remove other nutrients such as organic particles and inorganic phosphorus in aquaculture water. Therefore, in order to overcome the shortcomings of purifying aquaculture water through biofilm nitrification tanks, microorganisms and plants are usually used for joint purification. Aquaculture water. [0003] The existing method of using microorganisms and plants to joi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/32C02F3/34C02F3/30
CPCY02W10/10Y02W10/37
Inventor 陆开宏谢丽凤郑忠明
Owner NINGBO UNIV
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