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Method for rapidly extracting artemisinin

An artemisinin and fast technology, applied in organic chemistry and other directions, can solve the problems of large solvent consumption, low extraction efficiency, high energy and raw material consumption, etc. Effect

Inactive Publication Date: 2011-05-25
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are some defects in the industrial application of these types of extraction processes: organic solvent reflux extraction method is the commonly used artemisinin extraction technology at present, which has the disadvantages of excessive consumption of energy and raw materials, excessive solvent consumption, and low extraction efficiency. The process time is too long, the discharge of industrial pollution is large, the recovery rate is low, and the production cost is high. The microwave extraction method is thermally destructive. Artemisinin has thermal instability, which leads to a decrease in the biological activity and extraction rate of the extract; the ultrasonic extraction method has the disadvantages of cumbersome operation and low efficiency; although the supercritical fluid extraction method has the advantages of high extraction rate and less pollution, there are The problem of high cost is currently unable to realize industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Add 10 liters of ethyl acetate (analytically pure) to the extraction tank, then drop 2500g of fresh Artemisia annua (wet weight, the same below) into the extraction tank, extract for 8 minutes at normal temperature and pressure, filter, collect the extract, and add it to the extract Add 200g of activated carbon to decolorize, filter off the activated carbon, concentrate in vacuo at 35°C, recover ethyl acetate solvent, and obtain 16.12g of crude product, then add 120mL of n-hexane (analytical grade) and heat to boiling, dissolve, filter, and stand at room temperature for 2h to obtain Needle-like crystals were recrystallized with 80% edible ethanol to obtain 3.71 g of fine artemisinin; detected by gas chromatography, the purity of artemisinin was 98.7%, and the recovery rate of artemisinin was 87.1%.

Embodiment 2

[0020] Disposably add 10 liters of ethyl acetate (analytically pure) in the extraction tank, then drop into the fresh Artemisia annua raw material in the extraction tank, the total amount of raw material is 4500g, drop into, extract and filter in 3 times, drop into and extract 1500g each time, Extract at normal temperature and pressure for 5 minutes each time; collect the extract, add 400g of activated carbon to the extract for decolorization, filter off the activated carbon, concentrate in vacuo at 40°C, recover ethyl acetate, obtain 39.01g of crude product, add 200mL of 80% volume concentration of edible alcohol, heated to boiling, dissolved, filtered, and left at room temperature for 3 hours to obtain needle-shaped crystals, recrystallized with 65% volume concentration of edible alcohol to obtain 6.65g of fine artemisinin; detected by gas chromatography, the purity of artemisinin was 99.6 %, the recovery rate of artemisinin was 88.6%.

Embodiment 3

[0022] Add 10 liters of ethyl acetate at one time in the extraction tank, then drop into the fresh Artemisia annua raw material in the extraction tank, the total amount of raw material is 9000g, put in, extract, and filter in 5 times, and put in and extract 1800g each time, at normal temperature and normal temperature each time. Press and extract for 4 minutes; collect the extract, add 550g of activated carbon to the extract for decolorization, filter off the activated carbon, concentrate in vacuo at 50°C, recover ethyl acetate, obtain 69.11g of crude product, add 400mL of 70% volume concentration of edible alcohol, Heated to boiling, dissolved, filtered, and left at room temperature for 5 hours to obtain needle-shaped crystals, which were recrystallized with petroleum ether (analytical pure) to obtain 13.57 g of fine artemisinin; detected by gas chromatography, the purity of artemisinin was 99.8%, and artemisinin was recovered The rate is 89.5%.

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Abstract

The invention provides a method for rapidly extracting artemisinin, comprising the following steps: taking fresh and non-dry artemisia annua as an extraction raw material; extracting the artemisinin from the raw material using analytically pure ethyl acetate or chloroform or dichloromethane under the condition of normal temperature and normal pressure; filtering, collecting extracting solution, decolorizing by active carbon, concentrating and then recovering an extraction solvent to obtain a crude artemisinin product; and crystallizing and purifying the crude artemisinin product by edible ethanol or petroleum ether or n-hexane to finally obtain the artemisinin product with high purity up to over 95% and high recovery rate up to over 86%. The method has the advantages of being efficient and fast, low cost and applicability to industrial application.

Description

Technical field: [0001] The invention relates to the technical field of natural product separation engineering, in particular to a method for rapidly and efficiently extracting artemisinin from fresh and non-dried Artemisia annua at normal temperature and pressure. Background technique: [0002] Artemisinin is a sesquiterpene lactone peroxide isolated from Artemisia annua, and it is a specific drug for the treatment of malaria. Due to the resistance of malaria parasites to quinoline, artemisinin has become the drug of choice for the treatment of malaria, and the demand for artemisinin has risen sharply. Further pharmacological studies have proved that artemisinin and its derivatives have the effect of killing a variety of cancer cells, while causing little damage to normal cells, and there is no cross-resistance to traditional chemotherapy drugs. Therefore, the development of artemisinin and its derivatives into new anticancer drugs with high efficiency, low toxicity, low p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/20
Inventor 刘硕谦刘仲华田娜黄建安
Owner HUNAN AGRICULTURAL UNIV
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