Method for separating and purifying oligo-xylose by using simulated moving bed

Abstract

The invention relates to a method for separating and purifying oligo-xylose by using a simulated moving bed. The method comprises the following steps of: performing preprocessing such as decolorization, deinoning, condensation and the like on feed liquor obtained by performing enzyme hydrolysis on feed solution, namely hemicellulose; filtering and deoxidizing the preprocessed hemicellulose hydrolysis liquid with high purity water for later use; feeding the preprocessed hemicellulose hydrolysis liquid into a chromatographic separation device of the simulated moving bed to perform separation so as to obtain oligo-xylose crude liquor which is rich in oligo-xylose components; and performing reduced pressure evaporation concentration or excipient spray drying on the oligo-xylose crude liquor obtained by the separation to obtain oligo-xylose syrup or powder. In the method, the chromatographic separation device of the simulated moving bed (SMB) is used to extract the oligo-xylose, a plurality of adsorption posts is connected in series to form a closed loop, the positions of the inlet and outlet of each strand of materials are changed by continuously switching valves, and the relative movement of solid and liquid phases is realized, so that different components are separated and extracted. The method not only has the advantage of simple absorption operation of a fixed bed but also has the continuous operation capability of the moving bed, so that the method is suitable for large-scaled industrialized production.

Description

technical field [0001] The invention belongs to the technical field of simulated moving bed chromatographic separation, in particular to a method for separating and purifying xylooligosaccharides by using a simulated moving bed. Background technique [0002] Hemicellulose is one of the important components of plant materials, accounting for 15% to 35%. It is a heterogeneous polymer composed of several different types of monosaccharides, mainly five-carbon sugars. After pretreatment, it is The mother liquor hydrolyzed by xylanase mainly contains xylooligosaccharides, a small amount of xylose, arabinose and a small amount of glucose. Xylooligosaccharides are a mixture of 2 to 7 xylose molecules bonded by β-1,4 glycosidic bonds, with xylobiose, xylotriose and xylotetraose as the main components, also known as xylooligosaccharides. Its sweetness is about 40% of that of sucrose. It is a food functional factor that can proliferate bifidobacteria and improve the environment of int...

AI Technical Summary

Problems solved by technology

Both Chinese patent 200710054198.1 and Chinese patent 200410013840.8 adopt ultrafiltration and nanofiltration technology to separate and prepare xylooligosaccharides. Due to the limitation of membrane technology, the cut-off pore size of the membrane is not absolute, but the average value of a range, plus The concentration of the separated sugar solution is high, so to achieve a high degree of separation often requires a long column membrane length, making the separation process uneconomical

Both the enzyme separation method and the microbial fermentation method remove one or several components in the sugar liquid, the process is difficult to effectively control, and the use cost is high, which affects industrial production

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