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T4 lysozyme premix for animals and preparation method thereof

A premix, lysozyme technology, applied in the application, animal feed, additional food elements, etc., to achieve the effect of low cost, large practical application value and economic benefits

Inactive Publication Date: 2010-12-15
CHINA ANIMAL HUSBANDRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Before the present invention, there is no report on the method for large-scale preparation of T4 lysozyme derived from T4 phage and the corresponding premix product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] T4 lysozyme fermentation broth, plate and frame filter to remove mycelium, frame filtrate is ultrafiltered with 0.1 micron ceramic membrane, preferably hollow fiber column chromatography fine filtration with a pore size of 50,000 Daltons, according to the molecular weight of the T4 lysozyme product, preferably The organic nanofiltration membrane with a pore size of 2500 Daltons is concentrated by nanofiltration, and the nanofiltrate is refined and purified by cation exchange resin chromatography, preferably CM-Sephadex-C25 cation exchange resin. Enzyme powder was obtained by spray-drying the ion-exchange solution. The initial solid content of the spray-dried mother liquor was 4%, the inlet air temperature was 90°C, and the outlet air temperature was 60°C. Pass the enzyme powder through a 60-120 mesh sieve, weigh 62.5g of the sieved product, add 350g of corn gluten and 250g of bran that have passed through a 80-120 mesh sieve, add 50g of bentonite, 200g of CaCO3, and 87.5...

Embodiment 2

[0016] T4 lysozyme fermentation broth, plate and frame filter to remove mycelia, frame filtrate is ultrafiltered with 0.2 micron ceramic membrane, preferably hollow fiber column chromatography fine filtration with a pore size of 25,000 Daltons, according to the molecular weight of T4 lysozyme product, preferably An organic nanofiltration membrane with a pore size of 2500 Daltons is concentrated by nanofiltration, and the nanofiltrate is refined and purified by cation exchange resin chromatography, preferably Amberlite IRC-50 cation exchange resin. Enzyme powder was obtained by spray drying the ion exchange liquid, the initial solid content of the spray drying mother liquor was 8%, the inlet air temperature was 100°C, and the outlet air temperature was 80°C. Pass the enzyme powder through a 60-120 mesh sieve, weigh 118g of the sieved product, add 350g of zein and 200g of bran that have passed through a 80-120 mesh sieve, and add 100g of bentonite, CaCO 3 150g and 82g of zeolit...

Embodiment 3

[0018] T4 lysozyme fermentation liquid, plate and frame filter to remove mycelium, frame filtrate is ultrafiltered with 0.1 micron ceramic membrane, preferably hollow fiber column chromatography fine filtration with a pore size of 25,000 Daltons, according to the molecular weight of T4 lysozyme product, preferably The organic nanofiltration membrane with a pore size of 2500 Daltons is concentrated by nanofiltration, and the nanofiltrate is refined and purified by cation exchange resin chromatography, preferably CM-Sephadex-C25 cation exchange resin. Enzyme powder was obtained by spray-drying the ion-exchange solution. The initial solid content of the spray-dried mother liquor was 6%, the inlet air temperature was 95°C, and the outlet air temperature was 70°C. Pass the enzyme powder through a 60-120 mesh sieve, weigh 56g of the sieved product, add 350g of defatted soybean meal and 250g of wheat flour that have passed through a 80-120 mesh sieve, add 80g of bentonite, CaCO 3 20...

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Abstract

The invention belongs to the field of feed additives, and in particular relates to a pemix containing T4 lysozyme from T4 bacteriophage. The T4 lysozyme from the T4 bacteriophage replaces the traditional animal antibiotics, and the antibacterial growth-promoting feed premix containing the T4 lysozyme is prepared. A method for preparing the premix comprises the following steps of: centrifuging T4 lysozyme basic solution to remove impurities, performing membrane concentration, refining and purifying by using cation exchange resin, preparing protease powder by using a low-temperature spray drying method, screening the protease powder, adding auxiliary materials, and uniformly mixing at a high speed by a mixer to prepare the T4 lysozyme premix. The added auxiliary materials comprise soya beanmeal, bran, wheatmeal, wheat middling, bentonite, CaCO3, and zeolite. Experimental results show that the premix containing the T4 lysozyme has the effect of treating piglet diarrhea, which is obviously superior to that of a premix containing lysozyme from chicken egg albumin; and experimental results for feeding meat chickens and carp show that the premix containing the T4 lysozyme has the weightincreasing effect and feed utilization rate which are slightly superior to those of the premix containing lysozyme from chicken egg albumin.

Description

technical field [0001] The invention belongs to the field of feed additives, in particular to preparing a premix containing T4 lysozyme derived from T4 bacteriophage. Background technique [0002] my country has a vast territory and a large population. In recent years, the economy has developed rapidly, people's living standards have gradually improved, and the demand for high-quality animal protein products has continued to rise. This has led to the rapid development of the domestic animal health care product industry. The domestic food animal health care product market It is already the second largest market after the United States. [0003] With the introduction of various food safety measures in China, the corresponding development of safe, efficient and non-toxic animal feed additive products has become the development direction of the animal health care products industry. Lysozyme is an enzyme that can selectively dissolve the cell wall of target microorganisms and has...

Claims

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Application Information

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IPC IPC(8): A23K1/165A23K20/189
Inventor 余志强吴家鑫葛辛玫王华丽蔡健齐鹏郑应华张国栋曹芹宋敏
Owner CHINA ANIMAL HUSBANDRY IND
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