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Low-moisture solid culture and drying method for lactic acid bacteria

A technology of solid-state culture and drying method, which is applied in the field of preparation of live microbial preparations, can solve the problems of limited application area, high production cost, and lactic acid bacteria are not resistant to drying, and achieve the goal of reducing production cost, low price, and easy storage and transportation Effect

Active Publication Date: 2012-05-30
金泰得恒业(天津)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although lactic acid bacteria have a wide range of applications and market demand, because lactic acid bacteria are not resistant to drying, the current production mainly adopts freeze-drying, so the production cost is high, and its application is greatly limited

Method used

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  • Low-moisture solid culture and drying method for lactic acid bacteria
  • Low-moisture solid culture and drying method for lactic acid bacteria
  • Low-moisture solid culture and drying method for lactic acid bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1, prepare solid-state lactic acid bacteria preparation with low-moisture solid-state culture and drying method

[0031] Bacterial species: Enterococcus faecium, purchased from China General Microorganisms Collection and Management Center.

[0032] Utensils and equipment: 250mL triangular flask, 250mL canned bottle, 15L plastic fermentation container.

[0033] The main components of YPD liquid medium are: yeast extract 10g / L, peptone 20g / L, glucose 20g / L, pH 6.5.

[0034] The preparation method of YPD liquid medium is as follows:

[0035] 1) Dissolve 10g of yeast extract and 20g of peptone in 900mL of water, adjust the pH value, and make component I.

[0036] 2) Sterilize at 121°C for 30 minutes.

[0037] 3) Prepare 100 mL of glucose solution with a concentration of 200 g / L (10×), and sterilize separately at 115° C. for 20 min.

[0038] 4) Under aseptic conditions, add 100mL of sterilized 200g / L glucose solution into 900mL of component I, and mix well to ...

Embodiment 2

[0045] Embodiment 2, the detection of survival rate before and after drying of solid lactic acid bacteria preparation

[0046]Detect the survival rate of the solid lactic acid bacteria preparation before and after drying, the specific method includes the following steps:

[0047] (1) Determination of the number of lactic acid bacteria in the preparation before drying: Accurately weigh 10 g of the fermented material, put it into a 250 mL Erlenmeyer flask filled with 90 mL of sterile distilled water (containing several glass beads), vibrate at 200 rpm for 30 min in a shaker to make it Disperse evenly. According to the 10-fold dilution method, diluents of different concentrations were prepared, and 0.1 mL of the three appropriate dilutions were drawn and placed on sterile YPD plates, and three plates were made for each dilution. Inverted culture at 37°C for 24 hours, and count the colonies. Bacterial concentration calculation formula: Bacterial concentration (cfu / g) = 10 × dilu...

Embodiment 3

[0058] The storage characteristics of the lactic acid bacteria solid microbial agent that embodiment 3, low-moisture solid culture and drying method obtain

[0059] Get the finished lactic acid bacteria solid preparation after 500g drying, place 28 ℃ of constant temperature incubators, measure the viable count in the preparation every 7d, measuring method is with the step (1) of embodiment 3, and measuring result is as shown in table 2. According to the measurement results, the change curve of the number of live bacteria in the bacterial powder stored at room temperature is shown in figure 2 . Test results show that the lactic acid bacteria powder produced by the method of the present invention can not only be preserved for a long time at normal temperature, but also maintain a higher activity.

[0060] Table 2 Viable count results during the storage of finished lactic acid bacteria solid bacterial preparation

[0061]

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Abstract

The invention discloses a low-moisture solid culture and drying method for lactic acid bacteria. A low-cost agricultural product bran is adopted as a main raw material, and the low moisture state in the fermentation process is maintained by controlling the low moisture of the bran in the initial fermentation and absorbing water through dry soya bean meal in later fermentation, so the solid low-moisture culture for the lactic acid bacteria is obtained; and the solid low-moisture culture for the lactic acid bacteria is directly dried at the temperature of between 40 and 80 DEG C for 3 to 5 hours to form the dry solid culture for the lactic acid bacteria. The lactic acid bacteria obtained by fermentation can tolerate the high temperature in the drying process, have the survival rate of over 80 percent which is far higher than that of liquid culture bacteria in the drying process, are easy to store and transport, and have the viable bacteria survival rate of over 70 percent at normal temperature for 6 months. The method provides a technical basis for the large-scale production and application of the lactic acid bacteria, and is suitable for large-area promotion and application.

Description

technical field [0001] The invention relates to a method for preparing live microbial preparations in the field of biotechnology, in particular to a low-moisture solid-state culture and drying method for preparing lactic acid bacteria preparations by a solid-state fermentation method. Background technique [0002] In recent years, more and more researchers have paid attention to the role of lactic acid bacteria in maintaining the health of humans and higher animals. In terms of nutrition and physiology, lactic acid bacteria can improve the digestion and absorption of nutrients such as protein, lactose and calcium, and can produce a variety of vitamins for the body to digest and absorb; tract infection, relieve diarrhea and constipation, and has the effect of maintaining the balance of intestinal flora, can activate the function of macrophages, stimulate the body to produce an immune response, have immune activation and anti-tumor effects, etc., and can also reduce blood Amm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/225C12R1/46
Inventor 雷红升梁运祥付生慧游伟蔡君张东晓吴定心
Owner 金泰得恒业(天津)生物科技有限公司
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