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Preparation method of solid-phase extraction column by utilizing salbutamol molecular imprinting

A solid-phase extraction column and molecular imprinting technology are applied in the field of preparation of an albuterol molecularly imprinted solid-phase extraction column, and can solve the problems that the preparation method of an albuterol molecularly imprinted solid-phase extraction column has not yet been reported, and can achieve high recovery and reduce matrix. Effect, simple effect of preparation method

Inactive Publication Date: 2011-02-09
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no report yet about the preparation method of albuterol molecularly imprinted solid-phase extraction column

Method used

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  • Preparation method of solid-phase extraction column by utilizing salbutamol molecular imprinting
  • Preparation method of solid-phase extraction column by utilizing salbutamol molecular imprinting
  • Preparation method of solid-phase extraction column by utilizing salbutamol molecular imprinting

Examples

Experimental program
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Effect test

Embodiment 1

[0019] A method for preparing a salbutamol molecularly imprinted solid-phase extraction column: mix 100 mg of powdered salbutamol molecularly imprinted polymer with 10 ml of pure methanol, oscillate for 10 minutes and then sonicate for 20 minutes, mix evenly, and transfer it into an empty polypropylene solid-phase extraction column. Block them with membrane gaskets respectively, fill them evenly, and wash them on the column with 20ml of a mixed solution of acetic acid and methanol with a volume ratio of 1:9, then rinse with 10ml of pure methanol, and wash with N 2 Purging to remove methanol, vacuum drying that is.

[0020] Evaluation of the solid-phase extraction column: activate the salbutamol molecularly imprinted solid-phase extraction column with 5ml of methanol, then filter 1000ml of river water and load the sample at a flow rate of 1.0ml / min. Rinse with a mixed solution of 5 ml of methanol and water (1:10 by volume), and then elute with a mixed solution of 10 ml of metha...

Embodiment 2

[0022] A preparation method of salbutamol molecularly imprinted solid phase extraction column: mix 500mg of powdered salbutamol molecularly imprinted polymer with 20ml of pure ethanol, oscillate for 20 minutes and then sonicate for 10 minutes, mix evenly, transfer to polypropylene solid phase extraction empty column, and fill evenly After that, use 15ml of a mixed solution of trifluoroacetic acid and methanol with a volume ratio of 1:5 to wash the column several times, then rinse with 20ml of pure ethanol, and use Ar to remove the ethanol, and then dry it.

[0023] After the molecular imprinted solid phase extraction column was used 15 times, the recovery rate of albuterol was about 92.7%.

Embodiment 3

[0025] A preparation method of salbutamol molecularly imprinted solid-phase extraction column: mix 300mg of powdered salbutamol molecularly imprinted polymer with 15ml of pure acetonitrile, oscillate for 15 minutes and then sonicate for 15 minutes, mix evenly, transfer to polypropylene solid-phase extraction empty column, and fill evenly Finally, use 50ml volume ratio of formic acid and methanol mixed solution of 1:20 to wash the column several times, then wash with 15ml pure acetonitrile, and wash with N 2 Purging to remove acetonitrile, vacuum drying that is.

[0026] After the molecular imprinted solid phase extraction column was used 15 times, the recovery rate of albuterol was about 93.6%.

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Abstract

The invention discloses a preparation method of a solid-phase extraction column, in particular to a preparation method of a solid-phase extraction column by utilizing salbutamol molecular imprinting, which comprises the following steps: in terms of unit weight part and unit volume part, uniformly mixing 100 to 500mg of salbutamol molecular imprinted polymer and 5 to 10ml of pure organic solvent, and transplanting into a solid-phase extraction void column; washing on the column by utilizing an acid organic solvent after uniformly filling; washing by utilizing the pure organic solvent; blowing and eliminating the remaining organic solvent by utilizing inert gas; and drying to prepare the product. The preparation method of the invention is simple, the prepared solid-phase extraction column has the particularity matched with the salbutamol molecular imprinted polymer, and the prepared solid-phase extraction column has the advantages of high recovery rate of salbutamol and obvious matrix effect reduction.

Description

technical field [0001] The invention relates to a preparation method of a solid-phase extraction column, in particular to a preparation method of a salbutamol molecularly imprinted solid-phase extraction column. Background technique [0002] Salbutamol (Salbutamol, SAL) is used in the treatment of bronchial asthma due to its bronchodilator effect. In addition, because the drug can also promote the growth of muscle tissue, it is also used in the beef cattle industry to increase the proportion of lean meat in cattle, to replace the commonly used clenbuterol hydrochloride, and at the same time, it can be used as a stimulant to improve the physical fitness and performance of athletes . However, long-term or excessive use of these drugs is illegal and harmful to physiology, and serious toxic reactions will occur when the drugs are accumulated to a certain amount. Therefore, these drugs are prohibited from being taken by athletes and used in the production of beef. Generally sp...

Claims

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Application Information

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IPC IPC(8): B01J20/285B01D15/08B01J20/30B01J20/26
Inventor 杨敏莉沈蕊莲储晓刚王大宁
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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