Bioleaching method-uranium leaching from fungus
A technology of bioleaching and fungi, applied in the field of bioleaching-fungi leaching of uranium, can solve the problems of slow growth of bacteria, inability to reduce acid waste water, long metabolic time, etc., achieve fast growth, expand the scope of resource utilization, and grow condition requires simple effect
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[0032] A method of leaching uranium ore using fungi. The specific steps are:
[0033] (1) Enrichment and cultivation of fungi in uranium ore water, screening out fungi with strong acid production ability, and enriching and preserving them.
[0034] (2) Carry out uranium leaching research on the screened fungi, and screen out strains with good uranium leaching effect.
[0035] (3) Use cheap carbon sources such as straw to ferment fungi on a large scale.
[0036] (4) Take the culture or the filtered culture solution for leaching, and collect the uranium leachate.
[0037] (5) Determination of filtrate volume, uranium ion concentration, pH value and Eh value.
[0038] (6) Recover uranium from qualified filtrate.
[0039] IV embodiment
Embodiment 1
[0040] Example 1, take 5g of 745 ore 501 work area, which has been ground to -200 mesh, and put it in a 150mL Erlenmeyer flask, seal it with kraft paper, and sterilize under high pressure at 121°C for 30min, add 100mL of sterilized PDA medium and 0.5mL of Aspergillus niger mother liquor, and place 30 ℃ constant temperature air bath shaker culture. After cultivating for 7 days, take it out, filter, take a slag sample and dry it, grind it to -200 mesh, analyze the uranium content, and calculate the leaching rate. The results showed that the leaching rate reached 73.81%.
Embodiment 2
[0041] Example 2, take 5g of 745 mine 501 working area, which has been ground to -200 mesh, and put it in a 150mL Erlenmeyer flask, seal it with kraft paper, and sterilize under high pressure at 121°C for 30min, add 100mL of sterilized PDA medium and 0.5mL of Penicillium mother liquor, and place 30 ℃ constant temperature air bath shaker culture. After cultivating for 7 days, take it out, filter, take a slag sample and dry it, grind it to -200 mesh, analyze the uranium content, and calculate the leaching rate. The results showed that the leaching rate reached 73.42%.
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