Method for preparing polypeptide active substance from enzymolysis ginkgo protein

A technology of active substances and polypeptides, which is applied in the field of extraction and separation of plant active substances, can solve the problems of unfavorable absorption and achieve high purity

Active Publication Date: 2013-06-19
上海地湾电子科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, protein exists in the form of macromolecules, the number of amino acid monomers is greater than 100, and it does not contain various small peptides with less molecular weight, so it is not conducive to human body absorption

Method used

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  • Method for preparing polypeptide active substance from enzymolysis ginkgo protein
  • Method for preparing polypeptide active substance from enzymolysis ginkgo protein
  • Method for preparing polypeptide active substance from enzymolysis ginkgo protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for enzymatically hydrolyzing ginkgo protein to prepare polypeptide active substances, comprising the following steps:

[0034] The first step is to prepare defatted ginkgo powder: shell the fresh ginkgo, remove the skin, and beat it into a paste at a temperature lower than 20°C with a beater. For example, the temperature can be selected as 19°C, 18°C, 14°C, 10°C. ℃, 8℃, 6℃, 5℃, 4℃, -2℃, -6℃, -8℃, then use non-polar solvent or supercritical CO 2 Extract the oil, then freeze-dry at -10°C--50°C, for example, freeze-dry for 6-50 hours, the time can be 20 hours, 40 hours, infrared rapid moisture determination, the weight percentage of water is less than 5%, broken, Sieve to obtain defatted ginkgo powder with a particle size of 100-200 mesh;

[0035]The second step, enzymatic hydrolysis of defatted ginkgo powder: the defatted ginkgo powder and deionized water are mixed in a mass ratio of 1:5 to 1:15, for example, 1:8, 1:9, 1:11, 1 : 14, then add enzyme wherein, th...

Embodiment 2

[0042] Example 2 Enzymatic hydrolysis of ginkgo protein to prepare polypeptide active substances

[0043] A method for efficiently enzymatically hydrolyzing ginkgo protein to prepare polypeptide active substances, comprising the following steps: the first step is to prepare defatted ginkgo powder: shell 100g-200g of fresh ginkgo, and choose 100g, 120g, 150g, 180g, 200g, remove the coat Skin, use a beater at 5°C, 10°C, 12°C, 15°C, 18°C, 20°C to make a paste, and then use a non-polar solvent or supercritical CO 2 Extract oil, freeze-dry at -10°C-50°C, preferably -20°C-40°C, for example, choose -25°C, -30°C, -40°C, prepare defatted ginkgo powder, infrared rapid moisture determination, moisture < 5% (mass percentage), broken, sieved to obtain the defatted ginkgo powder with a particle size of 100 to 200 mesh and a protein content of 11.3% (mass percentage);

[0044] The second step of enzymatic hydrolysis of defatted ginkgo powder: mix the defatted ginkgo powder and deionized wat...

Embodiment 3

[0052] Example 3 Enzymatic hydrolysis of ginkgo protein to prepare polypeptide active substances

[0053] Shell 100g ginkgo fruit, remove the skin, and beat it into a paste with a beater at 15°C, then extract it with an equal volume of n-hexane for 3-5 times, remove all the oil, and freeze-dry the water layer at -35°C for 40 hours. Crush it into defatted ginkgo powder with a particle size of 100 to 200 meshes. The infrared rapid determination shows that the water content is 4.45%, and the protein content is 11.8%.

[0054] Weigh 20g of defatted ginkgo powder, add 100ml of deionized water, add 2g of neutral protease with an enzyme activity of 400,000 u / g, adjust pH=8, hydrolyze at a temperature of 50°C for 5h, centrifuge after hydrolysis, and collect the supernatant. The supernatant passes through a 0.45μm cellulose membrane, the filtrate first passes through a 10,000MW polysulfone membrane, the permeate passes through a 3,000MW polysulfone membrane, the retentate is frozen and...

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Abstract

The invention discloses a method for preparing ginkgo polypeptide. The method comprises the following the steps of: shelling a raw material; removing seed coats and grinding seeds into thick liquid; drying; degreasing; performing enzymatic hydrolysis on degreased ginkgo powder; performing membrane separation on enzymolysis liquid; collecting trapped fluid for concentrating; fractionally precipitating polysaccharide and polypeptide by using a solvent; washing polypeptide precipitate; and performing freeze drying so as to obtain polypeptide dry powder. The molecular weight of a polypeptide active substance is between 3,000 Dalton and 10,000 Dalton, the polypeptide content is 50 to 80 percent, the moisture content is less than 5 percent, and the granularity is between 100 meshes and 200 meshes. The polypeptide contains eight types of amino acids which are essential for human bodies; simultaneously, the content of lysine is more than 7 percent, the content of histidine is more than 8 percent, the content of serine is more than 11 percent, the content of aspartic acid is more than 14 percent, and the content of glutamic acid is more than 16 percent. In particularly, the content of the aspartic acid, the glutamic acid, the serine and the lysine in the polypeptide is remarkably higher than that of the same in the ginkgo raw material, so that a new method for deep processing of ginkgois found. The method has a simple process, is environmentally-friendly, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a technology for extracting and separating plant active substances, in particular to a method for enzymatically hydrolyzing ginkgo protein to prepare polypeptide active substances. Background technique [0002] In recent years, the research on peptides and proteins has progressed rapidly, especially natural peptides and proteins with physiological and pharmaceutical activities. At present, a large number of new components have been discovered. They not only play an important role in regulating body functions, but also play an important role in fighting AIDS. It is also active in diseases such as cancer, cardiovascular and cerebrovascular diseases. Ginkgo biloba not only contains flavonoids, terpene lactones, polysaccharides, trace elements, and oil. And contain more than 10% protein. Foreign scholars have found that there is a dynamic sperm-like substance ribonuclease in ginkgo. Deng Qianchun et al. isolated ginkgo active pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02
CPCY02P20/544Y02P20/54
Inventor 王成章陈西娟叶建中陈虹霞马维新周彬程耀波
Owner 上海地湾电子科技有限公司
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