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Method for detecting single molecule of single silicon nanowire in real time based on surface enhanced raman scattering effect

A surface-enhanced Raman and silicon nanowire technology, applied in Raman scattering, nanotechnology, nanotechnology and other directions, can solve the problem of no reports, and achieve the effects of cheap equipment and simple and fast preparation methods.

Active Publication Date: 2011-06-22
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are few reports on the use of silicon nanowires as the SERS detection substrate, and there are no reports on the single-molecule level detection.

Method used

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  • Method for detecting single molecule of single silicon nanowire in real time based on surface enhanced raman scattering effect
  • Method for detecting single molecule of single silicon nanowire in real time based on surface enhanced raman scattering effect
  • Method for detecting single molecule of single silicon nanowire in real time based on surface enhanced raman scattering effect

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1. Preparation of silicon nanowires uniformly loaded with silver nanoparticles on the surface

[0025] The silicon nanowires used in this example are prepared by using the existing method: using tin powder as a catalyst and thermally evaporating SiO powder; the details are as follows: weigh 3.0g SiO powder and 0.5g Sn powder, place them in a porcelain boat and mix Evenly, place the porcelain boat in the high temperature zone of the vacuum tube furnace, close the vacuum tube furnace; pump air until the pressure in the furnace chamber reaches 5×10 -2 After mbar, the mixed gas of argon and hydrogen is introduced until the pressure in the furnace is 350mbar, and the high temperature zone in the furnace is heated to 1360°C at a speed of 40°C / min, kept for 7-8h, and then cooled to room temperature. The diameter of the prepared silicon nanowire is about 100-500 nanometers, and the surface is covered with a silicon oxide layer. The crude silicon nanowires prepared in t...

Embodiment 2

[0029] Example 2. Real-time detection of a single molecule by a single silicon nanowire based on the surface-enhanced Raman effect

[0030] Use a capillary to absorb a small amount of the dispersed liquid of the sample prepared in Example 1 and drop it on a glass slide with a groove. After the ethanol volatilizes, the silicon nanowires are adsorbed on the glass surface, and then drop 5nM of R6G to the groove. Aqueous solution, cover the surface with a coverslip to prevent the solution from evaporating. Using renishaw confocal laser ramanspectroscopy to collect the spectrum of a single silicon nanowire to detect a single molecule, the simplified diagram of the detection system is shown in figure 2 As shown, the optical picture of detecting a single silicon nanowire is shown in image 3 shown. Select an objective lens with a magnification of 100 times (numerical aperture NA = 0.85, laser spot diameter of 0.5 μm, depth of field of 2 μm, after calculation, there will be no more...

Embodiment 3

[0031] Example 3. Real-time detection of single molecules by a single silicon nanowire based on the surface-enhanced Raman effect

[0032] According to the method of Example 2, the single silicon nanowire detects the signal of a single molecule in the solution. The difference is that in this embodiment, the same point on the single silicon nanowire is irradiated with continuous laser light, and 100 spectra are collected. , the spectral time interval is 1s, and the obtained Raman spectrum changes with time as shown in Figure 5 shown. Depend on Figure 5It can be seen that the spectrum has obvious brightening and fading phenomenon over time, which is a unique phenomenon of single-molecule Raman spectroscopy, which further proves that the sample can realize real-time detection of single molecules in the solution system.

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Abstract

The invention discloses a method for detecting a single molecule of a single silicon nanowire in real time based on a surface enhanced raman scattering (SERS) effect. The method comprises the following steps of: 1) dispersing the silicon nanowire of which a surface oxide layer is removed in aqueous solution of cetyltrimethyl ammonium bromide (CTAB), adding metal silver ion-containing solution into dispersion of the silicon nanowire under stirring, reacting for a period of time, centrifuging a product and collecting precipitates, and dispersing the obtained silicon nanowire on which the surface is loaded with silver particles in ethanol; and 2) dropwise adding ethanol dispersion of the silicon nanowire into a groove of a glass slide with the groove, adding aqueous solution of molecules to be tested into the groove after ethanol is volatilized, sealing the groove by using cover glass, irradiating the silicon nanowire at the groove by using laser of a certain wavelength, and collecting araman signal to realize real-time detection of the single molecule. The method is simple and fast and has a mild reaction condition, the prepared material has stable performance and higher SERS response, the detection of the single module in a solution system is realized, and a good platform is provided for researching the reaction of the single module in the solution system.

Description

technical field [0001] The invention relates to a method for real-time detection of a single molecule by a single silicon nanowire based on a surface-enhanced Raman effect. Background technique [0002] Single-molecule surface-enhanced Raman scattering (SERS), combined with the sensitivity of single-molecule fingerprinting, has become a powerful tool for studying the dynamics of single molecules adsorbed on surfaces and even single-molecule reactions in solution. However, in order to apply single-molecule detection to practice, a simple and convenient method for preparing a detection substrate with single-molecule enhancement capability is needed, and the detection in solution also requires a certain stability of the substrate. So far, it has been theoretically believed that aggregates of silver particles with a size in the range of 30-100 nm and a binding distance between particles in the range of 1-9 nm can be used as "hot spots" for SERS detection. People generally adopt...

Claims

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Application Information

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IPC IPC(8): G01N21/65C01B33/021B82B3/00
Inventor 张晓宏韩雪梅王辉欧雪梅李述汤
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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