Special compound enzyme for yeast hydrolysis and preparation method thereof

A yeast hydrolysis and compound enzyme technology, applied in biochemical equipment and methods, food preparation, enzymes, etc., can solve the problems of low wall breaking rate, low degree of hydrolysis, low yield, etc., and achieve rich nutrition, excellent functionality, The effect of high degree of hydrolysis

Active Publication Date: 2013-07-24
南宁庞博生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a yeast hydrolysis dedicated to the technical problems of limited hydrolysis raw materials, low wall breaking rate, low protein utilization rate, low degree of hydrolysis, low yield, and poor cut point of hydrolyzed substances in yeast hydrolysis. Compound enzyme and preparation method thereof

Method used

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  • Special compound enzyme for yeast hydrolysis and preparation method thereof
  • Special compound enzyme for yeast hydrolysis and preparation method thereof
  • Special compound enzyme for yeast hydrolysis and preparation method thereof

Examples

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Effect test

Embodiment 1

[0047] Preparation of β-glucanase: use soybean as raw material, crush, sterilize, add Trichoderma viride (Trichoderma viride) strain or Aspergillus niger (Aspergillus niger) strain, ferment for 3 days according to the conventional method, then extract and separate , ultrafiltration, freeze-drying, and crushing to obtain β-glucanase, which is stored for future use.

[0048] Preparation of mannanase: use soybean as raw material, pulverize, sterilize, add Trichoderma viride or Aspergillus niger, ferment for 3 days according to conventional methods, then extract, separate and ultrafilter , freeze-dried, and pulverized to obtain mannanase, which was stored for future use.

[0049] Preparation of glucoamylase: use soybean as raw material, pulverize, sterilize, add Trichoderma viride (Trichoderma viride) strain or Aspergillus niger (Aspergillus niger) strain, ferment for 3 days according to the conventional method, then extract, separate, ultrafilter, Freeze-dried and pulverized to ...

Embodiment 2

[0064] Preparation of β-glucanase: use soybean meal as raw material, pulverize, sterilize, add Trichoderma viride (Trichoderma viride) strain or Aspergillus niger (Aspergillus niger) strain, ferment for 4 days according to the conventional method, then extract and separate , ultrafiltration, freeze-drying, and crushing to obtain β-glucanase, which is stored for future use.

[0065] Preparation of mannanase: use soybean meal as raw material, pulverize, sterilize, add Trichoderma viride (Trichoderma viride) strain or Aspergillus niger (Aspergillus niger) strain, ferment for 4 days according to conventional method, then extract, separate, supersede Filter, lyophilize, and pulverize to obtain mannanase, which is stored for future use.

[0066] Preparation of glucoamylase: use soybean meal as raw material, pulverize, sterilize, add Trichoderma viride (Trichoderma viride) strain or Aspergillus niger (Aspergillus niger) strain, ferment for 4 days according to the conventional method,...

Embodiment 3

[0081] Preparation of β-glucanase: use wheat bran as raw material, pulverize, sterilize, add Trichoderma viride (Trichoderma viride) strain or Aspergillus niger (Aspergillus niger) strain, ferment for 3 days according to the conventional method, then extract, Separation, ultrafiltration, freeze-drying, and pulverization to obtain β-glucanase, which is stored for future use.

[0082] Preparation of mannanase: use wheat bran as raw material, pulverize, sterilize, add Trichoderma viride (Trichoderma viride) strain or Aspergillus niger (Aspergillus niger) strain, ferment for 3 days according to the conventional method, then extract, separate, Mannanase was obtained by ultrafiltration, freeze-drying and pulverization, which was stored for future use.

[0083] Preparation of glucoamylase: use wheat bran as raw material, pulverize, sterilize, add Trichoderma viride (Trichoderma viride) strain or Aspergillus niger (Aspergillus niger) strain, ferment for 3 days according to the convent...

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Abstract

The invention discloses a special compound enzyme for yeast hydrolysis and a preparation method thereof. The compound enzyme comprises the following enzyme components in parts by weight: 1-30 parts of beta-glucanase, 1-30 parts of mannase, 1-30 parts of saccharifying enzyme, 15-65 parts of endoenzyme, 1-10 parts of exoenzyme, 1-10 parts of flavourzyme and 1-10 parts of phosphodiesterase. The preparation method comprises the following steps: respectively fermenting, extracting, freeze-drying and pulverizing the beta-glucanase, mannase, saccharifying enzyme, endoenzyme, exoenzyme, flavourzyme and phosphodiesterase, and evenly mixing according to the proportion designed in the formula to obtain the special compound enzyme for yeast hydrolysis. Since multiple biological enzymes are coupled together for hydrolysis, the yeast hydrolysis time is shortened from 20-32 hours in the traditional way to 10-25 hours, the utilization ratio of the proteins is higher than 90%, the generation rate of amino acids is higher than 75%, the content of amino acids is higher than 0.83g / 100ml, and the total nitrogen content on dry basis is higher than 12.0%.

Description

technical field [0001] The invention belongs to the technical field of enzyme preparations, and relates to a special compound enzyme for yeast hydrolysis and a preparation method thereof. Background technique [0002] Yeast is rich in protein, glucan, mannan, vitamins, minerals and a large amount of glutathione, etc., and also contains 4 to 15% degradable ribonucleic acid (RNA), which can be decomposed by enzymatic technology Yeast cell wall dissolves protein, protease further decomposes protein into small peptides, amino acids, etc., phosphodiesterase decomposes ribonucleic acid to generate flavor nucleotides (I+G) and other substances, and obtains yeast extract products. It not only It has good nutritional effect and also has good health care effect. It is a new seasoning with three functions of seasoning, nutrition and health care. It can replace existing products such as monosodium glutamate, and has a huge market prospect. In natural condiments, yeast extract occupies ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/00A23L1/221A23L27/10
Inventor 刘汉灵黄月桂
Owner 南宁庞博生物工程有限公司
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