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Method and kit for detecting carbon dioxide bonding force

A detection method, carbon dioxide technology, applied in the field of inspection and measurement, can solve the problems of reduced coenzyme content, increase in content, and influence on the accuracy of carbon dioxide binding force, etc., and achieve good accuracy

Active Publication Date: 2011-08-17
CHANGCHUN HUILI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, carbon dioxide in the air will react with the reduced coenzyme, resulting in a decrease in the reduced coenzyme; in addition, the increase of endogenous substances in the sample to be detected, for example, some diseases will increase blood lactic acid, thereby dehydrogenating the lactic acid in the sample The increase of enzyme content, due to the action of lactate dehydrogenase, reduces the reduced coenzyme, which affects the accuracy of the detection of carbon dioxide binding capacity

Method used

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  • Method and kit for detecting carbon dioxide bonding force
  • Method and kit for detecting carbon dioxide bonding force
  • Method and kit for detecting carbon dioxide bonding force

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Kit preparation:

[0042] Weigh Tris at 30mmol / L and dissolve in distilled water, adjust the pH to 4.8-5.2 with 1N HCl, add sodium chloride, carbonic anhydrase and color developer respectively to obtain a mixed solution, divide the mixed solution into bottles, Freeze-drying, obtains dry powder reagent, comprises following composition in the described mixed solution:

[0043]

[0044] 40g / L of bovine serum albumin and 26mmol / L of sodium bicarbonate were mixed to obtain a standard solution.

Embodiment 2

[0046] Mix the kit prepared in Example 1 with distilled water and let it stand for 10 minutes to obtain the detection reagent;

[0047] Place the serum, the standard solution prepared in Example 1, and the detection reagent prepared above into the sample tray and reagent tray of the automatic biochemical analyzer, and the instrument automatically samples, mixes, and reacts according to the set parameters of 8 μl of sample and 240 μl of detection reagent. The reaction solution was obtained, and the incubation time was 300 seconds at 37°C, and the absorbance value at a wavelength of 546nm was detected, according to the calculation formula C 样 =A 样 / A 标 ×C 标 (26mmol / L), measure and calculate the concentration size of carbon dioxide binding force, wherein: C 样 is the concentration of the tested sample; A 样 is the sample absorbance value; C 标 Is the standard solution concentration (known); A 标 is the absorbance value of the standard solution.

Embodiment 3

[0049] Kit preparation

[0050] Prepare the first reagent:

[0051] Weigh Tris at a rate of 30 mmol / L and add distilled water to dissolve it, adjust the pH to 4.8-5.2 with 1N HCl, and add sodium chloride to obtain the first reagent.

[0052] Prepare the second reagent:

[0053] Dissolving carbonic anhydrase with 4% bovine serum albumin solution, dissolving the color reagent with 50% methanol, and then adding distilled water and stabilizer to obtain the second reagent.

[0054] The ingredients in the first liquid reagent and the shown second liquid reagent are as follows:

[0055]

[0056] Prepare standard solutions:

[0057] 40g / L of bovine serum albumin and 26mmol / L of sodium bicarbonate were mixed to obtain a standard solution.

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Abstract

The invention discloses a method and a kit for detecting carbon dioxide bonding force. The detecting method comprises the following steps of: mixing a sample to be detected of carbon dioxide bonding force and detection solution, and performing reaction to obtain reaction solution, wherein the detection solution comprises Tris-HCl buffer solution with pH value of 4.8 to 5.2, sodium chloride, carbonic anhydrase and color development agent; and detecting the absorbance value of the reaction solution under the wavelength of 540 to 550 nanometers, and obtaining the carbon dioxide bonding force by calculating. In the detection process, the carbon dioxide in the sample to be detected generates bicarbonate radicals under the action of the carbonic anhydrase, so that the pH value of the reaction solution is raised, and the color development agent develops color; and the carbon dioxide bonding force is calculated by detecting the absorbance value of the reaction solution. Compared with the prior art, color development reaction is used, reductive coenzyme is not needed, and the detected carbon dioxide bonding force is accurate.

Description

technical field [0001] The invention relates to the technical field of detection and determination, and more specifically relates to a detection method and a kit for carbon dioxide binding capacity. Background technique [0002] Carbon dioxide binding capacity refers to the content of carbon dioxide contained in bicarbonate in plasma. The determination of carbon dioxide binding capacity in serum or plasma is a routine item in clinical biochemical tests. The determination of carbon dioxide binding capacity can understand the acid-base balance in the human body. Respiratory acidosis, (emphysema, pulmonary fibrosis), respiratory muscle paralysis, bronchiectasis, pneumothorax, and airway obstruction; metabolic alkalosis, such as vomiting, adrenal hyperfunction, potassium deficiency, and excessive intake of alkaline drugs, etc. Both will cause acid-base poisoning, which will increase the carbon dioxide binding capacity; metabolic acidosis, such as uremia, shock, diabetic ketosis...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 董理
Owner CHANGCHUN HUILI BIOTECH CO LTD
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