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Spherical-cavity microelectrode array biosensor and preparation method thereof

A microelectrode array and biosensor technology is applied in the field of preparation of spherical cavity microelectrode array biosensors to achieve the effects of improving signal-to-noise ratio, high sensitivity and increasing loading capacity

Inactive Publication Date: 2011-09-14
CHANGSHU INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the deficiencies of the prior art, the present invention provides a spherical cavity microelectrode array biosensor, which comprehensively solves the problems of redox protein loading, activity, direct electron transfer rate between protein and electrode, signal-to-noise ratio, etc.

Method used

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  • Spherical-cavity microelectrode array biosensor and preparation method thereof

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Embodiment 1

[0034] For example, the platinum electrode was ultrasonically washed with acetone, ethanol, and water for 30 minutes and then stored in water. A polystyrene microsphere emulsion with a mass fraction of 1% was prepared with a 1:0.5 ethanol:water mixture as a solvent, and the size of the microspheres was 1500 nm. Using a mixture of water and ethanol with a volume ratio of 8.5:0.85 as the subphase, the polystyrene microsphere emulsion was slowly dripped onto the surface of the subphase. After standing for 15 minutes, the sliding barrier was compressed at a speed of 10 cm / min. When the membrane pressure reaches 10mN / m, keep the same membrane pressure for 30 minutes to stabilize the Langmuir membrane on the gas-liquid interface. The polystyrene microsphere array electrode was obtained by using the vertical pulling method to transfer it to the electrode at a speed of 1mm / min.

[0035] Mix 5mL of ethyl orthosilicate with 60mL of ethanol, put it in a constant temperature magnetic stirre...

Embodiment 2

[0038] The indium tin oxide electrode was ultrasonically washed with acetone, ethanol, and water for 30 minutes and then stored in water. A polystyrene microsphere emulsion with a mass fraction of 1% was prepared with a 1:1 ethanol:water mixture as a solvent, and the size of the microspheres was 1500 nm. Using a mixture of water and ethanol with a volume ratio of 8.5:1 as the subphase, the polystyrene microsphere emulsion was slowly dripped onto the surface of the subphase, and after standing for 20 minutes, the sliding barrier was compressed at a speed of 20 cm / min. When the membrane pressure reaches 14mN / m, keep the same membrane pressure for 30 minutes to stabilize the Langmuir membrane on the gas-liquid interface. The two-dimensional polystyrene microsphere array electrode is obtained by using the vertical pulling method to transfer it to the electrode at a speed of 1mm / min.

[0039] Prepare a mixture of 0.3mol / L zinc nitrate and 0.1mol / L citric acid, and heat the mixture to...

Embodiment 3

[0042] The glassy carbon electrode was ultrasonically washed with acetone, ethanol, and water for 30 minutes and then stored in water. A polystyrene microsphere emulsion with a mass fraction of 1% was prepared using a mixture of ethanol and water with a volume ratio of 1:1.5 as a solvent, and the size of the microspheres was 1500 nm. Using a mixture of water and ethanol with a volume ratio of 8.5:1.5 as the subphase, the polystyrene microsphere emulsion was slowly dripped onto the surface of the subphase, and after standing for 25 minutes, the sliding barrier was compressed at a speed of 30 cm / min. When the membrane pressure reaches 18mN / m, keep the same membrane pressure for 30 minutes to stabilize the Langmuir membrane on the gas-liquid interface. The two-dimensional polystyrene microsphere array electrode is obtained by using the vertical pulling method to transfer it to the electrode at a speed of 1mm / min.

[0043] Take 20mL of tetra-n-butyl titanate and add dropwise to 80mL...

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Abstract

The invention discloses a spherical-cavity microelectrode array biosensor, and the protein-modified spherical-cavity microelectrode array biosensor is obtained by dropwisely coating a phosphate buffer with dissolved protein on a surface of a spherical-cavity microelectrode array and vaporizing the buffer to dryness to form a membrane; the protein comprises various oxidases, various dehydrogenases, and all iron-containing protein. The signal to noise ratio of the detection signal of the biosensor is high; the protein load capacity is high, which greatly increases the sensitivity; and problems of redox protein load capacity, activity, direct electron transfer rate between the protein and the electrode, signal to noise ratio, and the like are solved.

Description

Technical field [0001] The invention relates to a biosensor and a preparation method thereof, in particular to a preparation method of a spherical cavity microelectrode array biosensor. Background technique [0002] Because electrochemical biosensors have the advantages of high sensitivity, good selectivity, rapid determination, simple structure, low cost, low energy consumption, easy miniaturization and integration, etc., they are considered to be used in occasions with high limitation requirements such as time efficiency and cost. One of the preferred technologies for biological detection. However, the detection limit of conventional electrodes is limited by the mass transfer rate of the substance to be tested and the charge and discharge current of the electric double layer on the electrode surface. -6 mol / dm 3 ) It is difficult to give an electrochemical response, which limits its application in trace analysis. The detection of trace substances is very important in the field...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 尹凡周群陈奠宇孙磊池小雷周丽娟周宇
Owner CHANGSHU INSTITUTE OF TECHNOLOGY
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