Preparation processes of flyblow chitin and chitosan and application of flyblow chitin in inducing trichoderma aureoviride T2 strains to produce chitinases

A fly maggot chitin and preparation technology, applied in the direction of enzymes, enzymes, microorganisms, etc., can solve the problems of poor product quality, environmental pollution, high energy consumption, etc., achieve low water content, shorten reaction time, and reduce environmental pollution. Effect

Inactive Publication Date: 2011-09-28
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the invention is to propose a preparation process of fly maggot chitin and chitosan, which solves the problems of high cost, high energy consumption and poor product quality in the traditional process of preparing chitin and chitosan with concentrated acid and concentrated alkali , and will cause serious environmental pollution

Method used

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  • Preparation processes of flyblow chitin and chitosan and application of flyblow chitin in inducing trichoderma aureoviride T2 strains to produce chitinases
  • Preparation processes of flyblow chitin and chitosan and application of flyblow chitin in inducing trichoderma aureoviride T2 strains to produce chitinases
  • Preparation processes of flyblow chitin and chitosan and application of flyblow chitin in inducing trichoderma aureoviride T2 strains to produce chitinases

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Experimental program
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Effect test

Embodiment 1

[0102] Present embodiment is the preparation technology of fly maggot chitin, prepares according to the following steps:

[0103] (1) Raw material pretreatment

[0104] Crush the dried fly maggots, wash them with water, wash off a large amount of protein components, and filter and collect the remaining maggot skins for later use;

[0105] (2) EDTA decalcification

[0106] Take by weighing the clean, dry fly maggot maggot skin that step (1) makes, soak 24h with (0.3mol / L) saturated EDTA solution, remove calcium, rear filter removes EDTA (recovery reuse), and maggot skin Wash with water until neutral;

[0107] (3) Dilute alkali to remove protein

[0108] Use 10% NaOH aqueous solution on the maggot skins treated in step (2) and put them in a boiling water bath for 2 hours to hydrolyze the protein. Stir continuously during the heating period and replace the lye every half hour. After the heating is over, remove the lye and wash with water until neutral , to obtain crude chitin...

Embodiment 2

[0112] Present embodiment is the preparation technology of fly maggot chitin, prepares according to the following steps:

[0113] (1) Raw material pretreatment

[0114] Crush the dried fly maggots, wash them with water, wash off a large amount of protein components, and filter and collect the remaining maggot skins for later use;

[0115] (2) EDTA decalcification

[0116] Take by weighing the clean, dry fly maggot maggot skin that step (1) makes, soak 20h with saturated EDTA solution, remove calcareous, rear filtration removes EDTA (recoverability reuse), and maggot skin is washed with water to neutrality;

[0117] (3) Dilute alkali to remove protein

[0118] Use 8% NaOH aqueous solution to the maggot skins treated in step (2), and put them in a boiling water bath for 1.5 hours to hydrolyze the protein. Stir constantly during the heating period, and replace the lye every half an hour. properties, to obtain crude chitin;

[0119] (4) Refined finished products

[0120] With...

Embodiment 3

[0122] Present embodiment is the preparation technology of fly maggot chitin, prepares according to the following steps:

[0123] (1) Raw material pretreatment

[0124] Crush the dried fly maggots, wash them with water, wash off a large amount of protein components, and filter and collect the remaining maggot skins for later use;

[0125] (2) EDTA decalcification

[0126] Take by weighing the clean, dry fly maggot maggot skin that step (1) makes, soak 30h with saturated EDTA solution, remove calcareous, rear filtration removes EDTA (recoverability reuse), and maggot skin is washed with water to neutrality;

[0127] (3) Dilute alkali to remove protein

[0128] Use 12% NaOH aqueous solution on the maggot skin treated in step (2) again, boil water bath for 3 hours, to hydrolyze protein, stir continuously during heating, and replace lye every half hour, after heating, remove lye, wash with water until neutral , to obtain crude chitin;

[0129] (4) Refined finished products

...

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Abstract

The invention discloses preparation processes of flyblow chitin and chitosan and application of the flyblow chitin in inducing trichoderma aureoviride T2 strains to produce chitinases, belonging to the technology field of biology. In the preparation process of the flyblow chitin, the traditional hydrochloric acid calcium removal process is mainly changed to an ethylene diamine tetraacetic acid (EDTA) calcium removal process, thereby reducing the environmental pollution caused by waste acid emission, avoiding the damage of concentrated acid to chitin molecular chains and obtaining the high-quality chitin product; and in the preparation process of the flyblow chitosan, the traditional process in which high-temperature concentrated alkali is refluxed for 4 hours is changed to a process in which a sodium hydroxide-amyl alcohol reaction system is adopted and microwave heating treatment is carried out twice, thereby improving the utilization of an alkali liquid, greatly shortening the reaction time, reducing energy consumption and obtaining the white powdery chitosan product with low water content and high deacetylation degree. The flyblow chitin provided by the invention can also be used as a carbon source so as to induce the trichoderma aureoviride T2 strains to produce the chitinases, wherein the induction effect is obvious.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation process of fly maggot chitin and chitosan and its application in inducing the production of chitinase by dark green xylinase T2 strain. Background technique [0002] Chitin (Chitin) is also known as chitin, chitin, chitin, polyacetylglucosamine, etc., and its chemical name is 1,4-2-acetylamino-2-deoxy-β-D-glucan, which is N- Acetyl-D-glucosamine (GlcNAc) is a linear polymer (C 16 h 26 o 10 N 2 )n. Widely present in the crustaceans of shrimps, crabs, insects, fungi (yeast, mold), algae and plant cell walls. Chitosan (Chitosan) is chitin off C 2 Acetyl products, also known as chitosan, deacetylated chitin, soluble chitin, polyglucosamine, etc., are chemically named poly(1,4)-2-amino-2-deoxy-B-D-glucose. [0003] At present, the domestic production of chitin and chitosan is mainly concentrated in coastal cities, most of which use shrimp and crab shells as raw materi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08C12N9/42C12R1/885
Inventor 徐秉良梁巧兰师桂英魏列新薛应钰杨顺义宋文勇
Owner GANSU AGRI UNIV
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